Research Article

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10/19/2011
12S rRNA; Bos gaurus; COI; Cyt b; Malayan gaur; PCR

PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various trials and combinations were tested to determine ... more

M.K.A. Rosli; A.S. Zamzuriada; S.M.F. Syed-Shabthar; M.C. Mahani; O. Abas-Mazni; B.M. Md-Zain
09/19/2013
16S rRNA; COI; Mitochondrial DNA gene sequences; Molecular phylogeny; Octopodidae species

Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a "catch-all" genus. The monophyly of octopus species in China's coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China's coastal waters using nucleotide sequences of two mitochondrial DNA genes: cytochrome c ... more

Z.M. Lü; W.T. Cui; L.Q. Liu; H.M. Li; C.W. Wu
06/21/2013
Biological control; COI; Genetic structure; Intraspecific divergence; Morphological variation; Placusa pinearum

Placusa pinearum, a newly described beetle species found living in pine shoot tunnels bored by the pine tip moth, Dioryctria rubella (Pyralidae), has potential as a vector to transport biological agents for controlling this moth pest of pine trees because of the high degree of niche overlap (co-occurrence) between them. In order to help determine the suitability of ... more

J.Y. Gao; B.Z. Ji; S.W. Liu
05/09/2014
16S rRNA; COI; Crustacean; Genetic polymorphism; Genetic variability; Macrobrachium

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and ... more

A.L. Guerra; A.V.B. Lima; R.V.Lucato Júnior; M.C. Chiachio; F.G. Taddei; L. Castiglioni
10/26/2011
COI; Cytochrome oxidase I; DNA barcoding; European bee-eater; Green bee-eater; Phylogenetics

DNA barcoding using mitochondrial cytochrome c oxidase subunit I (COI) is regarded as a standard method for species identification. Recent reports have also shown extended applications of COI gene analysis in phylogeny and molecular diversity studies. The bee-eaters are a group of near passerine birds in the family Meropidae. There are 26 species worldwide; five of them are found in Saudi ... more

I.A. Arif; H.A. Khan; M. Shobrak; J. Williams
04/27/2012
Apis mellifera L.; COI; DNA sequence diversity; Turkey

Mitochondrial DNA sequence variation can be used to infer honey bee evolutionary relationships. We examined DNA sequence diversity in the cytochrome C oxidase I (COI or Cox1) gene segment of the mitochondrial genome in 112 samples of Apis mellifera from 15 different populations in Turkey. Six novel haplotypes were found for the COI gene segment. There were eight variable ... more

F. Özdil; F. İlhan
12/08/2011
Cytokinin; Duplication; Maize (Zea mays); Phylogenetic analysis; RT-PCR; Two-component signal system

Cytokinins play many vital roles in plant development and physiology. In plants, cytokinin signals are sensed and transduced by the two-component signal system. This signaling cascade is typically composed of three proteins: a sensory histidine kinase, a histidine phosphotransfer protein, and a response regulator. Through a comprehensive genome-wide analysis of the maize (Zea mays ... more

Z.X. Chu; Q. Ma; Y.X. Lin; X.L. Tang; Y.Q. Zhou; S.W. Zhu; J. Fan; B.J. Cheng
10/25/2011
Cucumis sativus L.; Lipoxygenase; Phylogenetic analysis

Plant lipoxygenase (LOX) is involved in growth and developmental control processes, through the biosynthesis of regulatory molecules and defense responses to pathogens, wounding and stress. To date, few LOX proteins and little tissue expression profiling have been reported in detail for cucumber (Cucumis sativus L.). Recent completion of the cucumber genome sequence now permits ... more

S.Q. Liu; X.H. Liu; L.W. Jiang
07/25/2011
D-loop; Genetic diversity; Mitochondrial gene; Persian Gulf; Phylogenetic analysis; Sea turtles

Genetic diversity of sea turtles (hawksbill turtle) was studied using sequencing of mitochondrial DNA (mtDNA, D-loop region). Thirty dead embryos were collected from the Kish and Qeshm Islands in the Persian Gulf. Analysis of sequence variation over 890 bp of the mtDNA control region revealed five haplotypes among 30 individuals. This is the first time that Iranian haplotypes have been ... more

M. Tabib; H. Zolgharnein; M. Mohammadi; M.A. Salari-Aliabadi; A. Qasemi; S. Roshani; H. Rajabi-Maham; F. Frootan
08/03/2010
Cyclin; Gene duplication; Phylogenetic analysis; Zea mays

Cyclins are primary regulators of the activity of cyclin-dependent kinases and play crucial roles in cell cycle progression in eukaryotes. Although extensive studies have revealed the roles of some cyclins and underlying mechanisms in plants, relatively few cyclins have been functionally analyzed in maize. We identified 59 cyclins in the maize genome, distributed on 10 chromosomes; these ... more

X. Hu; X. Cheng; H. Jiang; S. Zhu; B. Cheng; Y. Xiang

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