Research Article

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Gene expression; Genetic variability; Isoenzymes; Ontogenetic stages

Sib-seedlings of 95 strains of the strictly autogamous grass Hordeum euclaston were analyzed by horizontal polyacrylamide gel electrophoresis for four isoenzyme systems at a specific ontogenetic stage. We found differences in the activity of some genes among individuals of this species. Hence, an ontogenetic analysis was carried out to investigate 12 strains at five ontogenetic ... more

J.E. Georg-Kraemer; C.A.S. Ferreira; S.S. Cavalli
03/09/2011
Backcross; Genetic variability; Inbreeding; Microsatellite markers; Parental genome

The limited number of papaya varieties available reflects the narrow genetic base of this species. The use of backcrossing as a breeding strategy can promote increases in variability, besides allowing targeted improvements. Procedures that combine the use of molecular markers and backcrossing permit a reduction of the time required for introgression of genes of interest and appropriate ... more

H.C.C. Ramos; M.G. Pereira; F.F. Silva; L.S.A. Gonçalves; F.O. Pinto; G.Ade Souza Filho; T.S.N. Pereira
02/22/2011
Cacao; Exoenzymes; Genetic variability; RAPD; Witches’ broom

We evaluated the genetic and physiological variability of Moniliophthora perniciosa obtained from healthy and diseased branches of cacao (Theobroma cacao) plants. The diversity of the isolates was evaluated by RAPD technique and by studies of virulence and exoenzyme production. The genetic variability of endophytic and pathogenic M. perniciosa was evaluated in ... more

T.G. Lana; J.L. Azevedo; A.W.V. Pomella; R.T.R. Monteiro; C.B. Silva; W.L. Araújo
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AFLP; Amazon region; Anopheles darlingi; Genetic variability; Malaria vector; Population differences

Anopheles darlingi is the most important malaria vector in Central and South America. After a dramatic reduction of malaria cases in the whole Brazilian territory, with the lowest abundance being attained by 1970, the disease resurged in the Amazon region, where it is now a great public health concern. Consequently, better knowledge about vector species became urgent. We ... more

L.M. Santos; R.A. Gama; A.E. Eiras; C.G. Fonseca
12/21/2010
Biodiversity conservation; Coefficient of similarity; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected from the wild in the region of Vitória da Conquista, Bahia ... more

C.B.M. Cerqueira-Silva; L.D.H.C.S. Conceição; E.S.L. Santos; C.B. Cardoso-Silva; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
10/26/2010
Coefficient of similarity; Conservation; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

In spite of the importance of and the considerable variability observed in Passiflora (Passifloraceae), little is known about the genetic diversity of most of the species of this genus. We evaluated the genetic diversity by RAPD markers in 18 genotypes of Passiflora trintae. The 15 primers generated 112 markers, 84% of which were polymorphic. The genetic distance ... more

C.B.M. Cerqueira-Silva; C.B. Cardoso-Silva; E.S.L. Santos; L.D.H.C.S. Conceição; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
09/08/2010
Anthracnose; common bean; Genetic variability; RAPD markers

Glomerella cingulata f. sp phaseoli is the sexual phase of the fungus Colletotrichum lindemuthianum, the causal agent of common bean anthracnose. This fungus is of great concern, because it causes large economic losses in common bean crops. RAPD markers of five populations of G. cingulata f. sp phaseoli from two Brazilian states were analyzed ... more

E.A. Souza; O.A. Camargo; J.M.A. Pinto
07/13/2010
Genetic variability; Heliconia; Molecular marker; Phylogenetic relationships; RAPD; Zingiberales

The family Heliconiaceae contains a single genus, Heliconia, with approximately 180 species of Neotropical origin. This genus was formerly allocated to the family Musaceae, but today forms its own family, in the order Zingiberales. The combination of inverted flowers, a single staminode and drupe fruits is an exclusive characteristic of Heliconia. Heliconias are ... more

L.P. Marouelli; P.W. Inglis; M.A. Ferreira; G.S.C. Buso
07/27/2010
Biomphalaria; Genetic variability; Polymorphism; RAPD-PCR; Schistosoma mansoni

Despite the implementation control programs, schistosomiasis continues to spread throughout the world. Among modern control strategies, vector control is currently being emphasized. Within this context, analysis of the genetic variability of intermediate host snails (Biomphalaria spp) is important because it allows identification of specific sequences of the genome of this ... more

A.L.D. Oliveira; D. Da Silva; B.C. Manzano; A.Z. Abdel-Hamid; M.Y. Marcelino; E.M. Zanotti-Magalhães; E.M. Zanotti-Magalhães; L.A. Magalhães; J.T. Ribeiro-Paes
04/20/2010
Douradinha; Genetic variability; Random amplified polymorphic DNA

Palicourea coriacea (Rubiaceae) is a herbaceous, perennial species typical of the Cerrado; it is popularly known as “douradinha”, because of its yellow flowers. It is utilized in popular medicine, mainly for the treatment of renal diseases. We used RAPD markers to evaluate the genetic structure of nine natural populations of P. coriacea, totaling 168 individuals, ... more

T.C.S. Barbosa; S.T. Sibov; M.P.C. Telles; T.N. Soares

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