Research Article

Related GMR Articles

08/17/2010
Gekko japonicus; Molecular cloning; Noggin2; Regeneration; Spinal cord

The cDNA encoding noggin2 protein was obtained from the brain and spinal cord cDNA library of Gekko japonicus. The size of the noggin2 transcript and its expression in different tissues were analyzed by Northern blot analysis. In situ hybridization revealed positive hybridization signals in both gray and white matter of the spinal cord. Changes in noggin2 ... more

Y. Liu; Y.L. Zhou; Y.Y. Qian; Y.J. Wang; F. Ding; X.S. Gu; M. Liu
02/04/2013
Cis-acting elements; GbDXS; GbGGPPS; Ginkgo biloba; Molecular cloning; Promoter

Ginkgolides are key pharmaceutical components in Ginkgo biloba leaves. 1-Deoxy-D-xylulose-5-phosphate synthase (GbDXS) and geranylgeranyl pyrophosphate (GbGGPPS) genes are critical genes involved in ginkgolide biosynthesis. In this study, the promoters of GbDXS and GGPPS, with 676 and 570 bp in length, respectively, were cloned by chromosome ... more

F. Xu; X.H. Huang; L.L. Li; G. Deng; H. Cheng; X.F. Rong; J.B. Li; S.Y. Cheng
08/29/2012
Bubalus bubalis; GC-rich; Molecular cloning; rDNA; rRNA

The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, including three rRNA genes and two ... more

C.Y. Pang; T.X. Deng; D.S. Tang; C.Y. Yang; H. Jiang; B.Z. Yang; X.W. Liang
03/06/2012
Molecular cloning; Penaeus monodon; Toll receptor

The black tiger shrimp (Penaeus monodon) is economically important in many parts of the world, including Thailand. Shrimp immunity is similar to that of other invertebrate organisms; it consists of an innate immunity system. Toll or Toll-like receptors (TLRs) play an essential role in recognizing the cleaved form of the cytokine Spätzle, which is processed by a series of ... more

W. Assavalapsakul; S. Panyim
11/19/2014
Bioactivity analysis; Giant panda; Interleukin 18; Molecular cloning

Interleukin 18 (IL-18), as a member of IL-1 superfamily, is an important pleiotropic cytokine that modulates Th1 immune responses. In this report, we cloned and identified a homolog of IL-18 in giant panda (Ailuropoda melanoleuca) (designated as AmIL-18) from peripheral blood mononuclear cells stimulated with lipopolysaccharide. The open readin g frame of AmIL-18 cDNA is ... more

Y. Yan; Q. Wang; L.L. Niu; J.B. Deng; J.Q. Yu; Y.Z. Zhang; J.X. Wang; M.M. Yin; X.M. Tan
02/19/2014
Crucian carp; Distant hybridization; Gene expression; jnk1 gene; Molecular cloning; Polyploidy

c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase superfamily. The allotetraploid crucian carp is a product of distant hybridization of female red crucian carp with male common carp. It is the first natural case of an allotetraploid with stable genetic characters, including fertility of both female and male animals. In this study, 2 jnk1 ... more

Y.M. Xiao; M.G. Jiang; Z.W. Luo; Y.H. Zhou; S. Wen; M. Wang; C. Zhang; S.J. Liu