Research Article

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RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The length of the cloned cDNA fragment was ... more

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Ailuropoda melanoleuca; ATP5G1; cDNA cloning; Giant panda; Overexpression

The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp encoding ... more

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Ailuropoda melanoleuca; Cloning; FABP4 and FABP5; Genomic sequence; Giant panda; Overexpression

Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction (RT-PCR) ... more

B. Song; Y.L. Hou; X. Ding; T. Wang; F. Wang; J.C. Zhong; T. Xu; J. Zhong; W.R. Hou; S.R. Shuai
10/18/2013
Anticancer activity; cDNA; Cloning; Giant panda; Overexpression; Purification; Ribosomal protein L24

The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain reaction technology ... more

Y.L. Hou; X. Ding; W. Hou; B. Song; T. Wang; F. Wang; J. Li; J. Zhong; T. Xu; B.X. Ma; H.Q. Zhu; J.H. Li; J.C. Zhong
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Sea Island cotton (Gossypium barbadense) is highly valued for its superior fiber qualities, especially fiber strength. Based on a transcript-derived fragment originated from transcriptome QTL mapping, a fiber strength related candidate gene of phosphatidylinositol 4-kinase cDNA, designated as GbPI4K, was first cloned, and its expression was characterized in the secondary ... more

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Alligator sinensis; cDNA cloning; Homology analysis; Insulin

Insulin is an important endocrine hormone that plays a critical physiological role in regulating metabolism and glucostasis in vertebrates. In this study, the complete cDNA of Alligator sinensis preproinsulin gene was cloned for the first time by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods; the amino acid sequence encoded and ... more

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Fusion protein; Giant panda; Interleukin-6/interleukin-2; Prokaryotic expression

To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriacetic acid column. ... more

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11/19/2014
Bioactivity analysis; Giant panda; Interleukin 18; Molecular cloning

Interleukin 18 (IL-18), as a member of IL-1 superfamily, is an important pleiotropic cytokine that modulates Th1 immune responses. In this report, we cloned and identified a homolog of IL-18 in giant panda (Ailuropoda melanoleuca) (designated as AmIL-18) from peripheral blood mononuclear cells stimulated with lipopolysaccharide. The open readin g frame of AmIL-18 cDNA is ... more

Y. Yan; Q. Wang; L.L. Niu; J.B. Deng; J.Q. Yu; Y.Z. Zhang; J.X. Wang; M.M. Yin; X.M. Tan
12/08/2011
cDNA cloning; hLIF; Oral mucous membrane; Overlap PCR

Complementary DNA (cDNA) is valuable for investigating protein structure and function in the study of life science, but it is difficult to obtain by traditional reverse transcription. We employed a novel strategy to clone human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA, which was directly isolated from the mucous membrane of mouth. The hLIF sequence, which is 609 bp ... more

Y.H. Cui; G.Q. Zhu; Q.J. Chen; Y.F. Wang; M.M. Yang; Y.X. Song; J.G. Wang; B.Y. Cao
03/27/2015
Bioinformatic analysis; cDNA cloning; Parathyroid hormone-related protein; Tissue expression analysis; Water buffalo

Parathyroid hormone-related protein (PTHrP) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. In this study, the full-length coding sequence of the water buffalo PTHrP gene was first isolated using reverse transcription-polymerase chain reaction. The protein was then subjected to molecular characterization using bioinformatic ... more

J. Liu; L.D. Qian; J.L. Huo; B.L. Bi; D.L. Li; S.F. Wang; T. Chen; L.J. Li; H.M. Mao; Y.W. Miao

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