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BoLA; Bovine; Candidate genes; Cattle; Molecular markers; Tick resistance

Losses caused by bovine tick burdens in tropical countries have a tremendous economic impact on production systems. Besides reducing production, this parasite can cause death in the most susceptible animals. The use of commercial acaricides has been the major method of control, but their misuse has led to tick resistance to many chemicals. More recently, vaccines have been used in ... more

M.L. Martinez; M.A. Machado; C.S. Nascimento; M.V.G.B. Silva; R.L. Teodoro; J. Furlong; M.C.A. Prata; A.L. Campos; M.F.M. Guimarães; A.L.S. Azevedo; M.F.A. Pires; R.S. Verneque
LEA proteins; qRT-PCR; Zea mays

We quantified and characterized the expression of heat-resistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two non-tolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they ... more

T. Andrade; E.V.R. Von Pinho; R.G. Von Pinho; G.E. Oliveira; V. Andrade; J.S. Fernandes
Gene structure; Hemoglobins; Immune response; qRT-PCR; Tegillarca granosa

Hemoglobin (Hb) is the major protein component of erythrocytes in animals with red blood, although it can serve additional functions beyond the transport of oxygen. The blood clam (Tegillarca granosa) is one of the few mollusks that has Hb, although the structure and function of molluskan Hbs remain unclear. We characterized two unique and highly compartmentalized blood clam ... more

Y.B. Bao; Q. Wang; X.M. Guo; Z.H. Lin
Hu sheep; LRH-1; qRT-PCR; Western blot

Liver receptor homologue 1 (Lrh-1) is a member of the nuclear receptor belonging to the second subfamily of the nuclear receptor family 5A (NR5A), also named NR5A2, which is important for lipid homeostasis, embryogenesis, and regulation of aromatics. The present study aimed to understand the sequence of ovine Lrh-1 and the expression traits in reproductive organ tissues. Initially, we ... more

L.H. Wang; W. Zhang; J.L. Ji; Q.X. Gao; S.H. Xiao; F. Wang
ELISA; Luteinizing hormone receptor (LHR); Non-gonadal tissues; qRT-PCR

Luteinizing hormone (LH) is an important glycoprotein hormone that regulates gonadal function in mammals and in turn regulates physiological status changes during the estrous cycle. The function of LH is mediated by luteinizing hormone receptor (LHR). In order to examine the expression patterns of the LHR gene in non-gonadal tissues during the 4 phases of the ovine estrous cycle ... more

L.H. Wang; W. Zhang; Q.X. Gao; F. Wang
Adipose; MDH1; MDH2; ME1; mRNA; Pig; qRT-PCR

Malate dehydrogenases 1 and 2 (MDH1 and MDH2), and malic enzyme 1 (ME1) play important roles in the Krebs cycle for energy metabolism. The mRNA abundance changes of MDH1, MDH2 and ME1 genes were measured across six different adipose tissues from the leaner Landrace and fatty Rongchang pig breeds using quantitative real-time PCR. The ... more

S.L. Zhou; M.Z. Li; Q.H. Li; J.Q. Guan; X.W. Li
Eriocheir sinensis; Na+-K+-ATPase; qRT-PCR; Salinity stress

Eriocheir sinensis is a euryhaline crab migrating from sea to freshwater habitats during the juvenile stage. We used quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the gene expression profile of Na+-K+-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities of 0 ... more

M. Sun; K. Jiang; F. Zhang; D. Zhang; A. Shen; M. Jiang; X. Shen; L. Ma
Expression pattern; Muscle growth; Porcine; qRT-PCR

Muscle growth and development is associated with remarkable changes in protein-coding and microRNA (miRNA) gene expression. To determine the expression patterns of genes and miRNAs related to muscle growth and development, we measured the expression levels of 25 protein-coding and 16 miRNA genes in skeletal and cardiac muscles throughout 5 developmental stages by quantitative reverse ... more

F.J. Wang; L. Jin; Y.Q. Guo; R. Liu; M.N. He; M.Z. Li; X.W. Li
Hsp; Low salinity stress; Portunus trituberculatus; qRT-PCR

Challenged by the low salinity, 4 parts per thousand (4 ppt), for 72h, the survivals of swimming crabs (Portunus trituberculatus) were collected as the screened group (SG, tolerant to low salinity). Aiming at identifying the mechanism of low salinity tolerance, quantitative real-time PCR was employed to investigate the expression profiles of 4 HSP genes (HSP60, HSP70 ... more

X.N. Bao; C.K. Mu; C. Zhang; Y.F. Wang; W.W. Song; R.H. Li; C.L. Wang