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Related GMR Articles

12/22/2011
Entosphenus; Lampetra richardsoni; Microsatellite; Pacific lamprey; Petromyzontidae; Western brook lamprey

The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish that is of conservation concern in North America and Asia. Data on Pacific lamprey population structure are scarce and conflicting, impeding conservation efforts. We optimized 12 polymorphic microsatellite loci for the Pacific lamprey. Three to 13 alleles per locus were observed in a sample of 51 fish collected ... more

E.K. Spice; T.A. Whitesel; C.T. McFarlane; M.F. Docker
10/31/2011
Hybrid; Microsatellite; Olive flounder; Starry flounder

An interspecific artificial hybrid was produced between two economically important aquaculture flatfish: olive flounder (Paralichthys olivaceus) and starry flounder (P. stellatus). This hybrid displays the rapid growth characteristic of the former and tolerance to low temperatures and low salinity of the latter, but the genetics of inheritance in this hybrid have not ... more

J.H. Kang; Y.K. Kim; J.Y. Park; C.M. An; M.M. Nam; S.G. Byun; B.I. Lee; J.H. Lee; T.J. Choi
12/19/2011
Cross-amplification; Lycoris radiata; Microsatellite; Molecular marker

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 ... more

S.Q. Xuan; J.Y. Zheng; H. Wang; P. Gao; S.B. Zhou; K. Liu; G.P. Zhu
10/19/2011
Genotyping; Heterozygote deficiency; Microsatellite; Null alleles; Parentage; White-tailed deer

Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for population management and conservation programs. We used empirical ... more

A.C. Kelly; N.E. Mateus-Pinilla; M. Douglas; M. Douglas; P. Shelton; J. Novakofski
08/16/2011
Harpadon nehereus; Microsatellite; Molecular marker

The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety positive clones from the (CA)15-enriched ... more

T.J. Xu; D.Q. Sun; H.Y. Li; R.X. Wang
09/12/2011
Genetic marker; Larus saundersi; Microsatellite; Polymorphism; Saunders’s gull

Nine polymorphic microsatellite loci were isolated, using tetranucleotide repeat oligonucleotide probes from an enriched DNA library of the globally “vulnerable” Saunders’s gull (Larus saundersi), collected from the Yancheng coastal wetland, one of the three remaining breeding sites in China. Six breast muscle tissues and 16 blood samples from 22 gulls and eight eggshell membrane ... more

H.X. Jiang; S.L. Ren; Y.Q. Hou; M. Yang; X.B. Wu
08/16/2011
Invasive species; Microsatellite; Polymorphism; Pseudorasbora parva

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The ... more

C. Zeng; Y. Gul; K. Yang; L. Cui; W.M. Wang; Z.X. Gao
07/25/2011
Coilia mystus; Conservation; Microsatellite; PIMA; RAPD-PCR enrichment

Coilia mystus is the most important harvested fish species in China; it inhabits quite different water environments during the different life history stages. Populations of C. mystus have dropped sharply due to overharvesting and water pollution. We developed eight microsatellite loci in C. mystus for conservation genetics studies. These new markers were tested ... more

J.Q. Yang; X.D. Zhou; D. Liu; Z.Z. Liu; W.Q. Tang
05/17/2011
Enriched genomic library; Epinephelus fuscoguttatus; Grouper; Microsatellite

Epinephelus fuscoguttatus is a commercially important marine fish species in southeast Asia. Due to overfishing and water pollution, this species has been declared as near-threatened. Thus, to provide information to help maintain and preserve the species, microsatellites were developed, using an enriched genomic library method. Thirty individuals were collected from the hatchery ... more

M.A.A. Mokhtar; M.N. Normah; S.V. Kumar; S.N. Baharum
05/10/2011
Abalone; Aquaculture; Haliotis diversicolor; Microsatellite

The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of alleles ranged from two to nine per locus, with an ... more

Y. Wang; F. Wang; Y.H. Shi; Z.F. Gu; A.M. Wang

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