Case Report

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C banding; Cichlidae; Crenicichla iguassuensis; Karyotype; Nucleolus organizer region banding; Perciformes

Three populations of the genus Crenicichla, namely Crenicichla iguassuensis, Crenicichla sp 1 and Crenicichla sp 2, from the Iguaçu River, were analyzed cytogenetically, and their nucleolus organizer regions, constitutive heterochromatin distribution and chromomycin A3 markings were studied. Karyotype analyses showed a diploid number of 48 ... more

S.M.H.K. Mizoguchi; A.L.B. Portela-Castro; I.C. Martins-Santos
Chromosome evolution; Cratylia argentea; Cratylia mollis; Cytogenetics; Karyotype; tropical forage

Cratylia argentea and C. mollis (Fabaceae-Papilionoideae) are legume shrubs native to the Cerrado and Caatinga, respectively. Both species show great resistance to drought and high nutritive value, which makes them a valuable forage resource in tropical regions. Cytogenetic studies were carried out on accessions of C. argentea and C. mollis from ... more

S.M. Vargas; G.A. Torres; F.S. Sobrinho; A.V. Pereira; L.C. Davide
Karyotype; Nucleolus organizer region band; Serrapinnus notomelas; Serrapinnus sp; Sexual chromosome ZZ/ZW

Four populations of Serrapinnus notomelas and one population of Serrapinnus sp.1, both belonging to the subfamily Cheirodontinae, were analyzed by Giemsa and silver nitrate impregnation techniques. We found 2n = 52 chromosomes for all populations, with interspecific differences in the karyotype formula; S. notomelas showed 16m + 22sm + 10st + 4a, with ... more

A.P. Santi-Rampazzo; P.B. Nishiyama; P.E.B. Ferreira; I.C. Martins-Santos
Animal cytogenetics; Karyotype; Lady beetle

The goal of the present study was to determine if simple methods, especially hot saline solution (HSS) and MspI and HaeIII restriction endonucleases, which do not require special equipments, may be helpful in studies of genetic variability in the lady beetle, Cycloneda sanguinea. The HSS method extracted the heterochromatin region, suggesting that it is ... more

E.M.D. Maffei; S.G. Pompolo
Heteroptera; Heteropycnosis; Karyotype; Lygaeidae; Nucleolar corpuscles; Nysius californicus

In Nysius californicus (family Lygaeidae, subfamily Orsillinae), a pest commonly known as the seed bug, the chromosome complement is 2n = 16 (12A + 2m + XY), testes are formed by seven seminiferous tubules covered by an orange-colored membrane, and spermatogenesis is cystic. At prophase, sex chromosomes are heteropycnotic and autosomes usually show a chiasma. At metaphase, ... more

H.V. Souza; H.E.M.C. Bicudo; M.M. Itoyama
Amazon region; Anopheles albitarsis complex; Karyotype; Malaria

Anopheles (Nyssorhynchus) albitarsis sensu lato is an important malaria vector in Brazil, especially in the Brazilian Amazon region. Chromosome preparations of fourth-instar larvae of A. albitarsis from Iranduba and Coari (AM) and Ilha Comprida (SP) were analyzed for karyotype determination and to improve cytogenetic identification of this species. ... more

M.Silva Rafael; I.Pereira do Santos-Junior; W.Pedro Tadei; M.Anice Mure Sallum; O.Paulo Forattini
Aplastodiscus; Hypsiboas; Karyotype; Nucleolus organizer region

Four species of Aplastodiscus and two species of Hypsiboas were cytogenetically compared. Aplastodiscus perviridis, A. cochranae, H. albomarginaus, and H. faber had 2n = 24 chromosomes, while A. albosignatus and A. leucopygius had 2n = 20 and 2n = 18 chromosomes, respectively. Aplastodiscus perviridis and A ... more

K.A. Carvalho; P.C.A. Garcia; S.M. Recco-Pimentel
C-banding; FISH; G-banding; Karyotype; Nucleolar organizer regions; Turtle
C.E.S. Zago
Heterochromatin; Karyotype; Non-disjunction; Rhamdia; Supernumerary chromosomes

Among catfish species of the genus Rhamdia reported for the Brazilian territory, R. quelen is the most widespread, being found in nearly all hydrographic basins of Brazil. Nowadays, R. quelen is a synonym for at least 47 other species in this genus, its taxonomic status still being controversial. The available cytogenetic reports show a wide variation in the ... more

C. Garcia; C. Oliveira; L.F. Almeida-Toledo
Chromosome evolution; Diploid number; Heterophrynus longicornis; Karyotype; Sex chromosome system

We analyzed mitotic and meiotic cells of a Brazilian amblypygid, Heterophrynus longicornis, using conventional and molecular cytogenetic techniques (Giemsa staining, C-banding, Ag-NOR, and FISH with rDNA probe). This is the first study that focuses solely on amblypygid chromosomes; it was undertaken to add data on cytogenetic knowledge of this group and contribute to the ... more

E. Paula-Neto; D. Araujo; L.S. Carvalho; D.M. Cella; M.C. Schneider