Research Article

Related GMR Articles

05/03/2011
Chromosome fusion; Cytogenetic; Diploid number; Nucleolar organizer region; Sex chromosome system

We made a cytogenetic analysis of four species of Oxyopidae and compared it with the karyotype data of all species of this family. In Hamataliwa sp, the mitotic cells showed 2n♂ = 26+X1X2 and telocentric chromosomes. The 2n♂ = 28, which has been described for only one oxyopid spider, is the highest diploid number reported for this family. Peucetia species exhibited ... more

L.M. Stávale; M.C. Schneider; A.D. Brescovit; D.M. Cella
11/03/2010
Cytogenetic; FISH; Passion flower; Pectinex

Cytological preparations for the fluorescent in situ hybridization (FISH) technique require cytoplasm-free metaphases, with well-spread chromosomes, for the localization of DNA sequences and chromosome mapping. We tested various procedures for FISH analysis of Passiflora cacaoensis, P. gardneri and hybrid F1 progeny of P. gardneri x P. gibertii ... more

M.M. Souza; J.D. Urdampilleta; E.R. Forni-Martins
11/11/2013
Acute myeloid leukemia; Cytogenetic; Hematopoietic stem cell transplantation; Molecular aberrations; Mutation

Different molecular aberrations can be discriminated into certain prognostic subgroups in cytogenetically normal acute myeloid leukemia (CN-AML) patients but their impact on allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains controversial and studies from Asian populations are lacking. Forty-two adult non-M3 AML patients receiving allo-HSCT from 2002 to 2009 in ... more

Y.C. Liu; H.H. Hsiao; P.M. Lin; W.C. Yang; C.S. Chang; T.C. Liu; J.F. Hsu; M.Y. Yang; S.F. Lin
11/12/2012
Chromosome heteromorphism; Cytogenetic; Recurrent pregnancy loss

Chromosomal heteromorphism is considered a variant of a normal karyotype, but it is more frequent in couples with repeated miscarriages. We investigated chromosomal heteromorphism in couples with repeated miscarriages in comparison with a control group. A total of 455 couples who applied to our genetic diagnosis laboratory in Diyarbakir, Turkey, were evaluated for chromosome ... more

H. Akbas; H. İsi; H. İsi; D. Oral; A. Turkyılmaz; S. Kalkanlı-Taş; S. Simsek; M. Balkan; M.N. Sakar; M. Fidanboy; M.N. Alp; T. Budak
07/16/2004
Chromosomal disorders; Genetic counseling

From 1986 to 2002, we examined the chromosomal composition of 916 patients attended by two genetic counseling services in the city of Pelotas, in the Brazilian State of Rio Grande do Sul, to determine the genetic causes of their disturbances. Patterns of G-banding using trypsin and Giemsa (GTG) and C-banding using barium and Giemsa (CBG) were studied using phytohemagglutinin M- ... more

A.C. Duarte; E. Cunha; J.M. Roth; G.L. Garcias; M.G. Martino-Roth; F.L.S. Ferreira
09/13/2012
c.3235G>A; COL1A1 gene mutations; Genetic counseling; Intrafamilial variability; Osteogenesis imperfecta

Osteogenesis imperfecta (OI) is a Mendelian disease with genetic heterogeneity characterized by bone fragility, recurrent fractures, blue sclerae, and short stature, caused mostly by mutations in COL1A1 or COL1A2 genes, which encode the pro-α1(I) and pro-α2(I) chains of type I collagen, respectively. A Brazilian family that showed variable expression of autosomal dominant ... more

M.V.D. Moraes; M. Milanez; B.V.P. Almada; V. Sipolatti; M.R.G.O. Rebouças; V.R.R. Nunes; A.N. Akel; M. Zatz; F.I.V. Errera; I.D. Louro; F. Paula
06/09/2014
9p deletion; Familial rearrangement; Fluorescence in situ hibridization; Genetic counseling; Multiplex ligation proble amplification subtelomeric; Trigonocephaly

We report two similarly affected cousins (children of monozygotic twin sisters) with phenotypic features consistent with 9p deletion syndrome, including dysmorphic craniofacial features (trigonocephaly, midface hypoplasia, upward-slanting palpebral fissures and long philtrum), intellectual disability and disorders of sex development. Initial cytogenetic examination showed normal ... more

L.S. Abreu; M.S. Brassesco; M.L.C. Moreira; J.M. Pina-Neto
03/31/2015
Assisted reproduction treatment; Familial balanced translocation; Genetic counseling; Reproductive outcome

Although it is known that parental carriers of structural chromosomal rearrangements are associated with recurrent pregnancy loss, subsequent natural pregnancies remain possible. We examined the reproductive outcome of a familial balanced translocation with t(3;6)(q12;q27). Karyotyping of the proband revealed 46,XY chromosomes with the balanced translocation t(3;6). The first 2 pregnancies ... more

H.G. Zhang; X.Y. Liu; Y. Hou; S. Chen; S. Deng; R.Z. Liu
08/27/2007
Cell suspension; Cytogenetic; DNA extraction; DNAzol

We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, ... more

M.R. Amorim; F.R. Vargas; J.C.Llerena Junior; M.S. Pombo-de-Oliveira
02/19/2008
Amphibia; Anthropogenic; Cytogenetic; Genetic diversity; Leptodactylidae; Random amplified polymorphic DNA

Cytogenetic and random amplified polymorphic DNA analyses carried out in the species Leptodactylus podicipinus, L. ocellatus, L. labyrinthicus, and L. fuscus from rural and urban habitats of the northwest region of São Paulo State, Brazil, showed that the karyotypes (2n = 22), constitutive heterochromatin distribution and nucleolus organizer region ... more

M.P. Arruda; E. Morielle-Versute

Pages