Research Article

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Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted. Partial 16S rRNA genes were amplified ... more

G.S. Magnani; L.M. Cruz; H. Weber; J.C. Bespalhok; E. Daros; V. Baura; M.G. Yates; R.A. Monteiro; H. Faoro; F.O. Pedrosa; E.M. Souza
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The increasing world production of biodiesel has resulted in an accumulation of crude glycerol as the major byproduct. This could be used as carbon source for industrial microbiology, with economic and environmental advantages for the biodiesel industry. We explored an Atlantic Rainforest soil sample to search for crude glycerol-degrading microorganisms. Microcosms of this soil were ... more

E.A.A. Duarte; G.V. Lacerda; T.A.S. de Oliveira; M. Brendel; L.L. Loguercio; J.Cde M. Cascardo
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The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane ... more

P. Li; S.F. Yang; B.B. Lv; K. Zhao; M.F. Lin; S. Zhou; X. Song; X.M. Tang
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Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a "catch-all" genus. The monophyly of octopus species in China's coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China's coastal waters using nucleotide sequences of two mitochondrial DNA genes: cytochrome c ... more

Z.M. Lü; W.T. Cui; L.Q. Liu; H.M. Li; C.W. Wu
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16S rRNA; Bacterial consortium; DNA extraction; Electrical energy; Inexpensive DNA protocol; Microbial fuel cell

Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the microbial communities in MFCs. ... more

B. Canto-Canché; M. Tzec-Simá; J.I. Vázquez-Loría; H. Espadas-Álvarez; B.H. Chí-Manzanero; R. Rojas-Herrera; R. Valdez-Ojeda; L. Alzate-Gaviria
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16S rRNA; Diversity; ERIC-PCR; High-throughput screening; Proteobacteria; RAPD

Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia ... more

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16S rRNA; Anaerobic biodigestion; Microbial community; Support medium

The performance of anaerobic filter bioreactors (AFs) is influenced by the composition of the substrate, support medium, and the microbial species present in the sludge. In this study, the efficiency of a slaughterhouse effluent treatment using three AFs containing different support media was tested, and the microbial diversity was investigated by amplified ribosomal DNA restriction ... more

M.I. Stets; R.M. Etto; C.W. Galvão; R.A. Ayub; L.M. Cruz; M.B.R. Steffens; A.C. Barana
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16S rRNA; COI; Crustacean; Genetic polymorphism; Genetic variability; Macrobrachium

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and ... more

A.L. Guerra; A.V.B. Lima; R.V.Lucato Júnior; M.C. Chiachio; F.G. Taddei; L. Castiglioni
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16S rRNA; Metagenomics; Reproductive disorders

The microbial community of the reproductive appara­tus, when known, can provide information about the health of the host. Metagenomics has been used to characterize and obtain genetic infor­mation about microbial communities in various environments and can relate certain diseases with changes in this community composition. In this study, samples of vaginal surface mucosal secretions were ... more

N.F. Rodrigues; J. Kästle; T.J.D. Coutinho; A.T. Amorim; G.B. Campos; V.M. Santos; L.M. Marques; J. Timenetsky; S.T. de Farias

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