Research Article

Related GMR Articles

03/20/2015
Heat stress; HSP70; Myocardial cells; Rats

To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro. After exposure to heat stress at 42°C ... more

H.B. Chen; X.C. Zhang; Y.F. Cheng; A. Abdelnasir; S. Tang; N. Kemper; J. Hartung; E.D. Bao
07/29/2014
Acrylonitrile; Apoptosis; Brain neuron; Neuron morphology; Rat

This study aimed to evaluate the effects of acrylonitrile (ACN) on neuronal morphology and apoptosis in rats. An ACN solution was administered to Wistar rats by gavage at doses of 0, 5, 10, or 20 mg/kg, 5 days a week for 13 weeks. The morphology of neurons and the presence of apoptosis was examined by light and electron microscope, DNA electrophoresis, immunohistochemistry, and terminal ... more

C.Z. Li; L.A. Tse; Y.L. Zhou; W. Fan; M. Li; I.T.S. Yu; T.Y. Jin
11/11/2014
Heat stress; Hsp60; In vivo and in vitro cell culture; Myocardial cell; Rat

To investigate the protective role of Hsp60 against stress damage and its role in the sudden death of stressed animals, changes in the levels of Hsp60 protein and hsp60 mRNA of myocardial cells in vivo and in vitro were studied. In addition, the relationship between Hsp60 expression and heat-induced damage was also studied. Rats were exposed to a temperature of ... more

Y.F. Cheng; J.R. Sun; H.B. Chen; A. Abdelnasir; S. Tang; N. Kemper; J. Hartung; E.D. Bao
08/20/2013
Heat shock factor-1; Heat shock protein 60; Heat shock protein messenger RNA; Heat stress; Myocardial cells

The mechanisms involved in sudden animal death due to acute heart failure during heat stress are not well understood. We examined the relationship between heat stress-induced variations of protective Hsp60 and expression of its regulatory factor, HSF-1, in heat-stressed primary myocardial cells of neonatal rats in vitro through cardiac enzyme detection, immunoblotting, ... more

R. Buriro; Y.J. Lv; I. Ali; S. Tang; Z.J. Liu; M. Zhang; A. Adem; J. Hartung; E.D. Bao
02/10/2009
Apoptosis; DNA damage; genomic instability; Inflammation; Isocyanates; Lung cancer

Lungs comprise the primary organ exposed to environmental toxic chemicals, resulting in diverse respiratory ailments and other disorders, including carcinogenesis. Carcinogenesis is a multi-stage phenomenon, which involves a series of genetic alterations that begin with genomic instability provoked by certain factors such as inflammation and DNA damage and end with the development of cancer. ... more

P.K. Mishra; A. Bhargava; G.V. Raghuram; S. Gupta; S. Tiwari; R. Upadhyaya; S.K. Jain; K.K. Maudar
12/08/2011
Apoptosis; Arg72Pro; Karyorrhexis; Micronuclei; rs56275308; TP53

Polymorphisms in the TP53 gene codon 72 (Arg72Pro) influence apoptosis induction and DNA damage repair. We evaluated how variants of protein p53 (p53Arg and p53Pro) affect cell death and DNA damage repair by analyzing the frequencies of karyorrhexis and micronuclei. There were significant differences in the frequency of karyorrhexis between the three p53 genotypes (Arg/Arg, Arg/ ... more

L. Pereira; M.R.S. Carvalho; C.G. Fonseca; S.S.S. Lima; E.M.M. Cerqueira; W. Jorge; M.C.L. Castro
10/18/2011
Apoptosis; CDV; Fas receptor; RT-PCR; Vero cells

We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero cells were used as a ... more

H.L. Del Puerto; A.S. Martins; G.F. Braz; F. Alves; M.B. Heinemann; D.S. Rajão; F.C. Araújo; S.F. Martins; D.R. Nascimento; R.C. Leite; A.C. Vasconcelos
09/09/2011
Angiotensin-II; Anti-aging; Apoptosis; IGF-1; klotho gene; PTH

Inactivation of the klotho gene in mice causes serious systemic disorders, resembling human aging. However, at the molecular level, its action mechanisms are not well understood. The stimulatory or inhibitory effects of cis- and trans-regulatory factors on the klotho gene expression are also still unclear. We studied the effects of intra- and extracellular factors on ... more

K. Turan; P. Ata
08/05/2011
Apoptosis; HSIL; LSIL; Micronucleus

We evaluated micronucleus and apoptosis occurrence among women with normal smears and women with different kinds of cervical abnormalities, i.e., inflammatory processes and low- and high-grade squamous intraepithelial lesions (N = 12, N = 10 and N = 27, respectively). The sample included 59 women who were seen at a public medical service for cervical cancer prevention in Feira de Santana, ... more

G.M.A. Aires; J.R.C. Meireles; P.C. Oliveira; J.L. Oliveira; E.L. Araújo; B.C. Pires; E.S.A. Cruz; N.F. Jesus; C.A.B. Pereira; E.M.M. Cerqueira
06/07/2011
A20 gene; Apoptosis; High glucose; HUVECs; Monocyte homing

Diabetes mellitus causes vascular lesions and may ultimately lead to atherosclerosis. One of the earliest steps in the development of atherosclerotic lesions is the adhesion of monocytes to endothelial cells of the vessel wall. It is currently unknown whether zinc finger protein A20 is able to protect endothelial cells from injury caused by high levels of glucose and monocyte homing. In ... more

C.L. Hou; W. Zhang; Y. Wei; J.H. Mi; L. Li; Z.H. Zhou; W. Zeng; D.J. Ying

Pages