Research Article

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03/27/2015
C-type lectin fold; Cloning; Metamorphosis; Mizuhopecten yessoensis; mRNA expression

C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. In this study, a putative C-type lectin fold (MyCLF) gene was identified from the Japanese scallop Mizuhopecten yessoensis. The full-length of MyCLF was 645 bp, encoding a polypeptide of 167 amino acids ... more

X.B. Bao; C.B. He; C.D. Fu; B. Wang; X.M. Zhao; X.G. Gao; W.D. Liu
01/23/2015
Capsicum annuum L.; CaRCCR gene; Cloning; Expression analysis; Stress response

Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum ... more

H.J. Xiao; J.H. Jin; W.G. Chai; Z.H. Gong
09/02/2004
Cysteine proteinase; Expression; Heterodera glycines; Inhibitory activity; Nematode; Pro domain peptide

Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propeptide to inhibit proteinase in its active ... more

F.B. Silva; J.A.N. Batista; B.M. Marra; R.R. Fragoso; A.Carolina S. Monteiro; E.L.Z. Figueira; M.Fátima Grossi-de-Sá
12/08/2011
Alternative splicing variant; DRD2 gene; Expression; Pig; Polymorphism

The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou, China) were selected to clone the gene and ... more

H.P. Xu; X.M. He; M.X. Fang; Y.S. Hu; X.Z. Jia; Q.H. Nie; X.Q. Zhang
02/28/2013
Adipocytokines; Expression; Insulin resistance; Polycystic ovary syndrome; Subcutaneous adipose tissue

We reviewed emerging evidence linking serum levels and adipose tissue expression of leptin and adiponectin in women with polycystic ovary syndrome (PCOS). Previous data obtained by our group from a sample of overweight/obese PCOS women and a control sample of normal weight controls, both stratified by BMI, were reanalyzed. Circulating levels of leptin and adiponectin were determined by ... more

S.B. Lecke; D.M. Morsch; P.M. Spritzer
09/12/2013
Expression; Heterosis; MRLC2 gene; Sequence analysis; Tianfu goat

To determine the molecular basis of heterosis in goats, fluorescence quantitative polymerase chain reaction (PCR) was performed to investigate myosin-regulatory light chain 2 (MRLC2) gene expression in the longissimus dorsi muscle tissues of the Tianfu goat and its parents, the Boer and Chengdu Ma goats. The goat MRLC2 gene was differentially expressed in the crossbreed ... more

H.G. Xu; G.Y. Xu; L. Wan; J. Ma
09/18/2013
Alcoholism; Atp5g3; Epigenetic; Expression; Mouse; Transcriptome

The mitochondrial ATP synthase, subunit c, isoform 3 gene (Atp5g3) encodes subunit 9, the subunit of the multisubunit enzyme that catalyzes ATP synthesis during oxidative phosphorylation in mitochondria. According to the Ensembl database, Atp5g3 in mice is located on chromosome 2 between 73746504 and 73749383 bp, within the genomic regions of two sets of quantitative ... more

Y. Huang; L. Wang; B. Bennett; R.W. Williams; Y.J. Wang; W.K. Gu; Y. Jiao
12/19/2012
Chitin deacetylase gene; Chitinase-3 gene; Expression; Hyriopsis cumingii; Potential functions

Chitin, the second most important natural polymer in the world, and its N-deacetylated derivative chitosan are found in a wide variety of organisms. These versatile biopolymers are associated with a broad range of biological functions. This article is the first to report the potential functions of 2 chitin metabolic enzyme genes from Hyriopsis cumingii. A chitinase-3 gene ( ... more

G.L. Wang; B. Xu; Z.Y. Bai; J.L. Li
12/21/2012
Expression; Heat shock protein 110; Heat shock protein 110 messenger RNA; Heat stress; In vitro; Primary myocardial cells of rat

We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases in aspartate transaminase, lactate dehydrogenase, and ... more

Z.J. Liu; Y.J. Lv; M. Zhang; Z.H. Yue; S. Tang; A. Islam; B. Rehana; E.D. Bao; J. Hartung
01/22/2013
Colon cancer; Expression; NPRL2; Real-time PCR; Tumor suppressor gene

Genetic and epigenetic factors affecting DNA methylation and gene expression are known to be involved in the development of colon cancer, but the full range of genetic alterations and many key genes involved in the pathogenesis of colon cancer remain to be identified. NPRL2 is a candidate tumor suppressor gene identified in the human chromosome 3p21.3 region. We evaluated the role of this ... more

B. Yogurtcu; I. Hatemi; I. Aydin; N. Buyru

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