Research Article

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11/28/2012
Ethylene receptor; Expression; Mangifera indica L.; Molecular modeling; Ripening

Ethylene induces characteristic ripening reactions in climacteric fruits through its binding to histidine-kinase (HK) receptors, activating the expression of ripening genes. Ethylene receptors have been found in Arabidopsis thaliana (Brassicaceae) and some fruits; number and expression patterns differ among species. In mango, only ethylene receptor ETR1 was known. We cloned ... more

C.A. Contreras-Vergara; N.A. Stephens-Camacho; G. Yepiz-Plascencia; G.A. González-Aguilar; A.A. Arvizu-Flores; E. Sanchez-Sanchez; M.A. Islas-Osuna
08/06/2012
Drosophila; Expression; GMR-GAL4; Wing

The GAL4/UAS binary system has been widely used in Drosophila melanogaster for ectopic expression of transgenes in a tissue-specific manner. The GMR-GAL4 driver, which expresses the yeast transcription factor GAL4 under the control of glass multiple reporter (GMR) promoter elements, has been commonly utilized to express target transgenes, specifically ... more

W.Z. Li; S.L. Li; H.Y. Zheng; S.P. Zhang; L. Xue
06/25/2012
Expression; FKBPs; Gene duplication; Maize

The receptors for the immunosuppression drugs FK506 and rapamycin are called FKBPs (FK506-binding proteins). FKBPs comprise a large family; they are found in many species, including bacteria, fungi, animals, and plants. As a class of peptidyl-prolyl cis-trans isomerase enzymes, the FKBP genes have been the focus of recent studies on plant stress tolerance and immunology. We ... more

W.W. Wang; Q. Ma; Y. Xiang; S.W. Zhu; B.J. Cheng
11/02/2008
Major histocompatibility complex; Poultry; Single nucleotide polymorphisms; TAP1; TAP2

TAP1 and TAP2 genes code for the two subunits of the transporter associated with antigen processing (TAP), and in chicken they are located between the two MHC class I genes. Using primers based on chicken sequences, the genomic regions corresponding to chicken TAP1 exons 6 to 7 and TAP2 exons 4 to 6 (which encode portions of the chicken TAP1 and ... more

L. Sironi; B. Lazzari; P. Ramelli; A. Stella; P. Mariani
01/18/2011
Allelic polymorphism; Balancing selection; Cynoglossus semilaevis; Major histocompatibility complex

Major histocompatibility complex (MHC) genes play an important role in the immune response of vertebrates. Allelic polymorphism and evolutionary mechanism of MHC genes have been investigated in many mammals, but much less is known in teleosts. We examined the polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of the half-smooth tongue sole ( ... more

T.J. Xu; Y.N. Sun; R.X. Wang
06/18/2015
Capra hircus; Domestic goat; DRA; Major histocompatibility complex

DRA encodes the alpha chain of the DR heterodimer, is closely linked to DRB and is considered almost monomorphic in major histocompatibility complex region. In this study, we identified the exon 2 of DRA to evaluate the immunogenetic diversity of Chinese south indigenous goat. Two single nucleotide polymorphisms in an untranslated region and one synonymous substitution in coding region were ... more

L.P. Chen; G.X. E; Y.J. Zhao; R.S. Na; Z.Q. Zhao; J.H. Zhang; Y.H. Ma; Y.W. Sun; T. Zhong; H.P. Zhang; Y.F. Huang
10/04/2012
Alternative splicing; Cancer-testis gene; Cloning; ODF3; ODF4

The outer dense fiber (ODF) genes encode proteins that co-assemble along the axoneme of the sperm tail. Recently, it was demonstrated that some ODF genes are aberrantly expressed in tumors, including prostate adenocarcinoma, basal cell carcinoma, and chronic myeloid lymphoma. We cloned ODF3 and ODF4 cDNA from the testis of a patient suffering from ... more

S. Ghafouri-Fard; S. Ghafouri-Fard; M.H. Modarressi
08/29/2012
BAC library; Bubalus bubalis; Cloning; Pulse-field gel electrophoresis

River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52, ... more

N.B. Stafuzza; C.A. Abbey; C.A. Gill; J.E. Womack; M.E.J. Amaral
12/18/2014
Bioinformatic analysis; Calcium-dependent protein kinases; Cloning; TgCDPK6; Toxoplasma gondii

Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very ... more

N.Z. Zhang; S.Y. Huang; D.H. Zhou; Y. Xu; J.J. He; X.Q. Zhu
12/04/2014
Cloning; Date palm; Maturase K (matK); Ribulose bisphosphate carboxylase (rbcL); Sequencing; Taxonomy

DNA barcoding is a promising tool for species identification at the molecular level. The barcoding system is well established for species differentiation in animals, while it is less common in plants. We evaluated 2 barcoding regions, maturase K (matK) and ribulose bisphosphate carboxylase (rbcL), to compare species of Palmae according to amplification success, ... more

A. Naeem; A.A. Khan; H.M.N. Cheema; I.A. Khan; A. Buerkert

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