Research Article

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05/15/2002
Apis mellifera; Honey bee; Intranuclear acid phosphatase activity; Post-embryonic development

We report nuclear acid phosphatase activity in the somatic (intra-ovariolar and stromatic) and germ cells of differentiating honey bee worker ovaries, as well as in the midgut cells of metamorphosing bees. There was heterogeneity in the intensity and distribution of electron dense deposits of lead phosphate, indicative of acid phosphatase activity in the nuclei of these tissues, during ... more

Cda Cruz Landim; R.Daniele Reginato; R.Lucia More de Moraes; V.Melo Cavalcante
01/25/2003
Africanized; Apis mellifera; Effective reproduction; Fertility; Reproduction; Varroa

Varroa destructor reproductive success is considered an important character for determining the resistance of honey bees to this mite parasite. However, most of the published data are not comparable due to the different methods of ascertaining and reporting reproduction. A recently published technique that involves reconstructing mite families in older worker brood gives ... more

M.H. Corrêa-Marques; L.Medina Medina; S.J. Martin; D. De Jong
03/31/2006
Apis mellifera; Bioinformatics; doublesex gene; Evolution of genes; Molecular genetics; Sex determination; Sexual development; Transcription factors

A comparison of the most conserved sex-determining genes between the fruit fly, Drosophila melanogaster, and the honey bee, Apis mellifera, was performed with bioinformatics tools developed for computational molecular biology. An initial set of protein sequences already described in the fruit fly as participants of the sex-determining cascade was retrieved from the Gene ... more

A.S. Cristino; A.Mendes do Nascimento; Lda Fontour Costa; Z.Luz Paulin Simões
08/09/2005
Apis mellifera; Evolution of parasite-host relations; Integumental wounds; Multiple brood infestation; Pathogen invasion; Varroa destructor

The ectoparasitic bee mite, Varroa destructor, is highly adapted to its natural and adopted honey bee hosts, Apis cerana and Apis mellifera. Adult females perforate the integument of bee pupae in such a way that they and their progeny can feed. We examined the wounds that founder females made, and usually found one, and rarely up to three, integumental wounds ... more

G. Kanbar; W. Engels
03/31/2005
Apis mellifera; Honey bees; Hygienic behavior; Removing; Uncapping

Most research on hygienic behavior has recorded the time taken by the colony to remove an experimental amount of dead brood, usually after one or two days. We evaluated the time that hygienic (H) and non-hygienic (NH) honey bees take to uncap and remove dead brood in observation hives after the brood was killed using the pin-killing assay. Four experimental colonies were selected as ... more

M.Alejandra Palacio; J.Manuel Flores; E. Figini; S. Ruffinengo; A. Escande; E. Bedascarrasbure; E. Rodriguez; L.Segui Gonçalves
10/13/2009
Africanized; Apis mellifera; Bee nutrition; Bradford; Protein diets

The superiority of Africanized over European honey bees in tropical and subtropical regions of the New World is both well documented and poorly understood. As part of an effort to try to understand the process by which the displacement of European bees occurred, we examined the ability of these two types of bees and of hybrids between the two to convert natural and artificial diets into ... more

F.A. Cappelari; A.P. Turcatto; M.M. Morais; D. De Jong
06/30/2009
Africanized honey bees; Apis mellifera; body fluid; Carniolan honey bees; Hygienic behavior; pin-killing method

In Apis mellifera, hygienic behavior involves recognition and removal of sick, damaged or dead brood from capped cells. We investigated whether bees react in the same way to grouped versus isolated damaged capped brood cells. Three colonies of wild-type Africanized honey bees and three colonies of Carniolan honey bees were used for this investigation. Capped worker brood cells aged 12 ... more

K.P. Gramacho; L.S. Gonçalves
06/23/2009
Africanized honey bee; Apis mellifera; Hygienic behavior; Observation hives

The hygienic behavior of honey bees is based on a two-step process, including uncapping and removing diseased, dead, damaged, or parasitized brood inside the cell. We evaluated during periods of 1 h the time that hygienic and non-hygienic colonies of Africanized honey bees spend to detect, uncap and remove pin-killed brood using comb inserts with transparent walls placed in observation hives. ... more

M.M. Morais; T.M. Francoy; R.A. Pereira; D. De Jong; L.S. Gonçalves
06/16/2009
Apis mellifera; Biodiversity; Geometric morphometrics; mtDNA; species identification; Wing morphometrics

Though the replacement of European bees by Africanized honey bees in tropical America has attracted considerable attention, little is known about the temporal changes in morphological and genetic characteristics in these bee populations. We examined the changes in the morphometric and genetic profiles of an Africanized honey bee population collected near where the original African swarms ... more

T.M. Francoy; D. Wittmann; V. Steinhage; M. Drauschke; S. Müller; D.R. Cunha; A.M. Nascimento; V.L.C. Figueiredo; Z.L.P. Simões; D. De Jong; M.C. Arias; L.S. Gonçalves
12/19/2013
Apis mellifera; Hemolymph; Nutrition; Pollen substitute; Protein diets

Pollen substitute diets are a valuable resource for maintaining strong and health honey bee colonies. Specific diets may be useful in one region or country and inadequate or economically unviable in others. We compared two artificial protein diets that had been formulated from locally-available ingredients in Brazil with bee bread and a non-protein sucrose diet. Groups of 100 newly- ... more

M.M. Morais; A.P. Turcatto; R.A. Pereira; T.M. Francoy; K.R. Guidugli-Lazzarini; L.S. Gonçalves; J.M.V. de Almeida; J.D. Ellis; D. De Jong

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