Research Article

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04/10/2015
Crustacean; Developmental expression; Macrobrachium nipponense; Male-specific lethal 3

In this study, male-specific lethal 3 homolog (Mnmsl3) was cloned and characterized from the freshwater prawn Macrobrachium nipponense (Crustacea: Decapoda: Palaemonidae) by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnmsl3 showed high-sequence homology to the insect Msl3 and contained a conserved chromatin organization modifier ... more

Y.P. Zhang; S.M. Sun; H.T. Fu; X.P. Ge; H. Qiao; W.Y. Zhang; Y.W. Xiong; S.F. Jiang; Y.S. Gong; S.B. Jin
05/09/2014
16S rRNA; COI; Crustacean; Genetic polymorphism; Genetic variability; Macrobrachium

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and ... more

A.L. Guerra; A.V.B. Lima; R.V.Lucato Júnior; M.C. Chiachio; F.G. Taddei; L. Castiglioni
11/27/2014
Expression pattern; Muscle growth; Porcine; qRT-PCR

Muscle growth and development is associated with remarkable changes in protein-coding and microRNA (miRNA) gene expression. To determine the expression patterns of genes and miRNAs related to muscle growth and development, we measured the expression levels of 25 protein-coding and 16 miRNA genes in skeletal and cardiac muscles throughout 5 developmental stages by quantitative reverse ... more

F.J. Wang; L. Jin; Y.Q. Guo; R. Liu; M.N. He; M.Z. Li; X.W. Li
04/10/2015
Expression pattern; Gill; HSP90; Megalobrama amblycephala; Nitrite exposure

Heat shock protein 90 (HSP90), a highly conserved and multi-functional molecular chaperone, plays an essential role in cellular metabolism and stress response. In this study, HSP90 cDNA named MaHSP90 was cloned from Wuchang bream (Megalobrama amblycephala) gills by using rapid amplification of cDNA ends. The full-length MaHSP90 cDNA is 2674 bp and consists of a 3',5'-untranslated ... more

S.M. Sun; J. Zhu; X.P. Ge; C.F. Zhang; L.H. Miao; X.J. Jiang
11/30/2010
Crustacean; Macrobrachium; Phylogenetic relathionship; Polymorphism; Prawn; RAPD-PCR

The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We analyzed three species of this genus (M. ... more

A.L. Guerra; A.V.B. Lima; F.G. Taddei; L. Castiglioni
12/13/2013
Embryos; Expression pattern; Pectoral muscle; Pekin duck; Tissue sections

To confirm the entire developmental process and transition point of embryonic Pekin duck pectoral muscle, and to investigate the association between pectoral muscle development and their regulating genes, anatomical and morphological analyses of embryonic Pekin duck skeletal muscles were performed, and the expression patterns of its regulating genes were investigated. The anatomical ... more

L.H. Gu; T.S. Xu; W. Huang; M. Xie; W.B. Shi; S.D. Sun; S.S. Hou
05/06/2013
Expression pattern; Insulin-like growth factors; Muscle tissue

Insulin-like growth factors (IGFs) are regulators that modulate the proliferation and differentiation of muscle tissues. We quantified the messenger RNA (mRNA) expression of IGF-I, IGF-II, and type I and II IGF receptors (IGF-IR and IGF-IIR) in muscle tissues including the breast, leg, and myocardium during an early postnatal development growth stage (post-hatching weeks 1-8) in ducks. ... more

C.L. Song; H.H. Liu; J. Kou; L. Lv; L. Li; W.X. Wang; J.W. Wang
04/02/2013
Characterization; Cloning; Expression pattern; Spodoptera litura; Trehalase

The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and function of treh ... more

Q. Zou; P. Wei; Q. Xu; H.Z. Zheng; B. Tang; S.G. Wang
11/18/2013
Genetic diversity; Macrobrachium nipponense; Microsatellite marker; Yellow river

To assess the genetic status of this species, the genetic diversity of wild Macrobrachium nipponense from seven geographic locations in the Yellow River basin were investigated using 20 polymorphic microsatellite DNA loci. The genetic diversity between populations was indicated by the mean number of alleles per locus and mean observed heterozygosity (H) and the expected H, which ... more

H. Qiao; D. Lv; S.F. Jiang; S.M. Sun; Y.S. Gong; Y.W. Xiong; S.B. Jin; H.T. Fu
10/30/2013
Cathepsin L; Embryo development; Macrobrachium nipponense; Ovarian maturation; Yolk protein degradation

We identified the cDNA sequence of cathepsin L (CatL) in Macrobrachium nipponense, designated as MnCatL, for the first time. The MnCatL cDNA, isolated from the ovary, was 1710 bp in length, containing a 31-bp 5'-untranslated region, a 650-bp 3'-untranslated region, and an open reading frame of 1029 bp, encoding a 342-amino acid polypeptide with a predicted molecular mass of 37.7 ... more

W. Zhao; L. Chen; F. Zhang; P. Wu; E. Li; J. Qin

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