Research Article

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Cloning; Distribution; Isoforms; Macrobrachium nipponense; Retinoid X receptor

Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, five RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, and RXRS3, containing six domains from A to F, were cloned from ... more

Z. Li; W.Q. Wang; E.F. Zhang; G.F. Qiu
05/18/2015
Broad-complex; cDNA; Macrobrachium nipponense; Metamorphosis; Real-time quantitative polymerase chain reaction

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors. In this study, we isolated the full-length cDNA of a BR-C homolog from the testes of the oriental river prawn (Macrobrachium nipponense), according to established expressed sequence tag information, using the rapid amplification of cDNA ends ... more

S.F. Jiang; Y.P. Zhang; S.M. Sun; Y.S. Gong; Y.W. Xiong; H. Qiao; W.Y. Zhang; S.B. Jin; H.T. Fu
04/30/2015
Female sterile homeotic (fsh); Gene cloning; Gene expression; Macrobrachium nipponense

The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its ... more

W.Y. Zhang; S.F. Jiang; Y.W. Xiong; H.T. Fu; H. Qiao; S.M. Sun; Y.S. Gong; S.B. Jin
03/20/2015
Differentially expressed genes; Macrobrachium nipponense; Ovary; Reproduction-related genes; Testis

This study utilized high-throughput RNA sequencing technology to identify reproduction- and development-related genes of Macrobrachium nipponense by analyzing gene expression profiles of testis and ovary. More than 20 million 1 x 51-bp reads were obtained by Illumina sequencing, generating more than 7.7 and 11.7 million clean reads in the testis and ovary library, respectively. ... more

H. Qiao; Y.W. Xiong; S.F. Jiang; H.T. Fu; S.M. Sun; S.B. Jin; Y.S. Gong; W.Y. Zhang
12/28/2015
Androgenic gland; Macrobrachium nipponense; MicroRNA; Sex-determination; Targeted genes

The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China. The androgenic gland produces hormones that play crucial roles in the differentiation of crustaceans to the male sex. MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing important biological and metabolic processes. However, currently, there is no ... more

S.B. Jin; H.T. Fu; S.F. Jiang; Y.W. Xiong; H. Qiao; W.Y. Zhang; Y.S. Gong; Y. Wu
05/25/2016
Crustacean; Expression pattern; Feminization-1 homolog b (Fem1b); Macrobrachium nipponense; Post-larval; RT-qPCR

Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. ... more

N.M.A. Rahman; H. Fu; H. Qiao; S. Jin; H. Bai; W. Zhang; F.W. Jiang; G. Liang; S. Sun; Y. Gong; F.F. Jiang; Y. Xiong; Y. Wu; N.M.A. Rahman; H. Fu; H. Qiao; S. Jin; H. Bai; W. Zhang; F.W. Jiang; G. Liang; S. Sun; Y. Gong; F.F. Jiang; Y. Xiong; Y. Wu
08/29/2016
Cloning; Expression pattern; Macrobrachium nipponense; Nitric oxide synthase; Post-larvae; RT-qPCR

Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of l-arginine to l-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the oriental river prawn Macrobrachium nipponense is ... more

N.M.A. Rahman; H.T. Fu; S.M. Sun; H. Qiao; S. Jin; H.K. Bai; W.Y. Zhang; G.X. Liang; Y.S. Gong; Y.W. Xiong; Y. Wu; N.M.A. Rahman; H.T. Fu; S.M. Sun; H. Qiao; S. Jin; H.K. Bai; W.Y. Zhang; G.X. Liang; Y.S. Gong; Y.W. Xiong; Y. Wu
08/29/2016
Cytosolic manganese superoxide dismutase; Expression analysis; KK-42; Macrobrachium nipponense

Imidazole derivative KK-42 is a well-known regulator of insect growth. KK-42 pretreatment has been shown to promote the survival of Macrobrachium nipponense infected with Aeromonas hydrophila, possibly via activation of superoxide dismutase (SOD). In this study, the cytMnSOD gene was cloned from the hepatopancreas of M. nipponense using the rapid ... more

W.F. Wang; L.M. Mu; X.S. Wu; H. Yang; Q.J. Ning; W.F. Wang; L.M. Mu; X.S. Wu; H. Yang; Q.J. Ning
03/31/2006
Apis mellifera; Bioinformatics; doublesex gene; Evolution of genes; Molecular genetics; Sex determination; Sexual development; Transcription factors

A comparison of the most conserved sex-determining genes between the fruit fly, Drosophila melanogaster, and the honey bee, Apis mellifera, was performed with bioinformatics tools developed for computational molecular biology. An initial set of protein sequences already described in the fruit fly as participants of the sex-determining cascade was retrieved from the Gene ... more

A.S. Cristino; A.Mendes do Nascimento; Lda Fontour Costa; Z.Luz Paulin Simões
12/04/2013
Bulked segregant analysis; Neotropical fishes; RAPD; Sex determination

Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual ... more

I.G. Almeida; P. Ianella; M.T. Faria; S.R. Paiva; A.R. Caetano

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