Research Article

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10/10/2013
Alkaline phosphatase; Cantharidin; Glutathione S-transferase; Helicoverpa armigera

The molecular and biochemical effects of an insecticidal toxin extracted from Meloidae beetles were investigated on Helicoverpa armigera. The toxin was identified as cantharidin, a well-known natural compound produced by beetles of family Meloidae and Oedemeridae. Furthermore, the effect of the toxin on the metabolic enzymes alkaline phosphatase (ALP) and glutathione S- ... more

R.A. Khan; M. Rashid; D. Wang; Y.L. Zhang
10/15/2012
ELISA; Luteinizing hormone receptor (LHR); Non-gonadal tissues; qRT-PCR

Luteinizing hormone (LH) is an important glycoprotein hormone that regulates gonadal function in mammals and in turn regulates physiological status changes during the estrous cycle. The function of LH is mediated by luteinizing hormone receptor (LHR). In order to examine the expression patterns of the LHR gene in non-gonadal tissues during the 4 phases of the ovine estrous cycle ... more

L.H. Wang; W. Zhang; Q.X. Gao; F. Wang
03/14/2012
ELISA; IFN-γ; IL-2; Immunopharmacological activity; Piperine; RT-PCR

Piperine, an amide isolated from Piper species (Piperaceae), has been reported to exhibit central nervous system depression, anti-pyretic and anti-inflammatory activity. Immunomodulatory and anti-tumor activity of piperine has been demonstrated in mouse carcinomas. However, there is little information available concerning the effect of piperine on humans. We evaluated the ... more

S. Chuchawankul; N. Khorana; Y. Poovorawan
08/07/2006
Alkaline phosphatase; Gene regulation; Neurospora crassa; Phosphate sensing

The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three non-specific alkaline phosphatase activities, one of them possibly being a novel putative enzyme, which is not responsive to ... more

T. Junior; A. Rossi
03/15/2013
Aggrecan; Alkaline phosphatase; Collagen; Osteocalcin; rhBMP-2; TGF-β1

We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphatase, type I collagen, type II ... more

J.Y. Park; Y.S. Yoon; H.S. Park; S.U. Kuh
06/28/2013
ELISA; Pcdh1; Rat polyclonal antibody; Western blotting

Protocadherins constitute a large family belonging to the cadherin superfamily; they function in various tissues of a wide variety of multicellular organisms. However, their functions and expression modes are still unknown in many of these species and tissues. We developed a fast and low-cost method to produce polyclonal antibody against chicken protocadherin 1 (Pcdh1) that could be used ... more

Y.H. Li; X.H. Zhao; M. Li; C.Q. Yang; L. Wang; J.T. Lin
09/12/2014
CFP-10 protein; ELISA; Mycobacterium tuberculosis; Rv2626c protein; Serodiagnosis

We constructed a Mycobacterium tuberculosis vector expressing CFP-10 and Rv2626c to examine the expression of these proteins in Escherichia coli as well as their immunoreactivity. The CFP-10 and Rv2626c genes were amplified from tuberculosis H37Rv genomic DNA using polymerase chain reaction. They were ligated into the expression vector PET30a and expressed in E. coli ... more

Z.Y. Zhu; D. Zhang; H.B. Wang; J.Z. Xiao; Y.F. Qiu; L. Yan; D. Chen; A.G. Liu; X. Yang
06/18/2015
Ageing; cortisol; ELISA; Feces; Immunoglobulin; Reindeer

Immunoglobulin and cortisol levels are good indicators of well-being and living status in animals. In this study, the concentrations of fecal immunoglobulins A ([IgAF]), G ([IgGF]), and M ([IgMF]), and cortisol ([cortisolF]) were examined by enzyme-linked immunosorbent assay in reindeer of the Greater Khingan Mountains of Inner Mongolia, China ... more

Y.J. Yin; C.Y. Nie; W.S. Liu; Q. Zou; J.C. Zhai; H.S. Han; H.P. Li
06/11/2015
Adhesion; Aeromonas hydrophila; ELISA; flgN; Mini-Tn10Km

Adhesion to the host mucus is a crucial step in the early infection stage of pathogenic bacteria. To investigate the mechanisms of the adhesion of Aeromonas hydrophila to its host mucus, a mutant library was constructed using the mini-Tn10 transposon mutagenesis system. Of 276 individual colonies, the mutant strain with the most attenuated adhesion ability in this study ... more

X. Jiang; Y.X. Qin; G.F. Lin; L. Huang; B. Huang; W.S. Huang; Q.P. Yan
04/22/2015
ELISA; Monitoring of blood concentration; Renal transplantation; Tacrolimus

This study aimed to 1) analyze the results of tacrolimus blood concentration monitored in patients after renal transplantation, 2) observe and establish an optimal therapeutic window for patients, and 3) provide evidence for the clinical and rational use of drugs. Tacrolimus blood concentration was determined by enzyme-linked immunosorbent assay. A total of 1824 cases were obtained from ... more

C.Y. Wang; X. Xu; M.C. Li; Q. Li; S.G. Ji

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