Research Article

Related GMR Articles

Ctenomyidae; Cytochrome b; Evolution; Molecular phylogeny

The genus Ctenomys (Rodentia: Ctenomyidae) shows several taxonomic inconsistencies. In this study, we used an integrative approach including DNA sequences, karyotypes, and geometric morphometrics to evaluate the taxonomic validity of a nominal species, Ctenomys bicolor, which was described based on only one specimen in 1912 by Miranda Ribeiro, and since then neglected. ... more

J.F.B. Stolz; G.L. Gonçalves; L. Leipnitz; T.R.O. Freitas
Cytochrome b; Detection dog; Fecal DNA; Microsatellite; Molecular ecology

We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20'S, 47°17'W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples ... more

M.L. Oliveira; J.M.B. Duarte
Cytochrome b; Endangered species; Fishery product identification; Polymerase chain reaction-restriction fragment length polymorphism

Endangered angelsharks and a protected dolphin species are illegally traded in Brazil. In this study, we determined prospective molecular markers for detecting these species in the trade of angelshark carcasses and ‘dolphin’ eyeball amulets. We compiled publicly available as well as new and unpublished cytochrome b (cyt b) DNA sequences for species involved in these ... more

L.H.O. Falcão; M.A.A. Furtado-Neto; R. Maggioni; V.V. Faria
Cancer; DmtDNA4977; In situ PCR; Kerns-Sayre syndrome deletion mutation; Mitochondria; Mitochondrial DNA; Real-time PCR

Levels of mtDNA4977 deletions (DmtDNA4977) have been found to be lower in tumors than in adjacent non-tumoral tissues. In 87 cancer patients, DmtDNA4977 was detected by multiplex polymerase chain reaction (PCR) amplification in 43 (49%) of the tumors and in 74 (85%) of the samples of non-tumoral tissues that ... more

M.Angela C. Dani; S.U. Dani; S.P.G. Lima; A. Martinez; B.M. Rossi; F. Soares; M.A. Zago; A.J.G. Simpson
African Americans; American Caucasians; Brazilians; Mitochondrial DNA; Y-chromosome

We have previously shown evidence of strong sex-biased genetic blending in the founding and ongoing history of the Brazilian population, with the African and Amerindian contribution being highest from maternal lineages (as measured by mitochondrial DNA) and the European contribution foremost from paternal lineages (estimated from Y-chromosome haplogroups). The same phenomenon has been ... more

V.F. Gonçalves; F. Prosdocimi; L.S. Santos; J.M. Ortega; S.D.J. Pena
Melipona mondury; Melipona rufiventris; Meliponini; Mitochondrial DNA; Polymorphic site; RFLP

Within the Meliponini, a widely distributed group of stingless bees, Melipona rufiventris has been considered as a single, cohesive species. Recently, analysis of morphological characters led to the splitting of this species into two species, M. mondury and M. rufiventris. The former occurs in the Atlantic Rain Forest ranging from Santa Catarina to Bahia ... more

G.S. Barni; R. Strapazzon; J.C.V. Guerra; G. Moretto
allele-specific polymerase chain reaction; Bovine; maternal inheritance; Mitochondrial DNA

The Canchim (5/8 Charolais + 3/8 Zebu) beef cattle breed was developed at Southeast-Embrapa Cattle to take advantage of hybrid vigor and to combine the higher growth rate and beef quality of Charolais with tropical adaptations of Zebu. The development of three lineages (old, new, and crossbred) has increased its genetic basis. The genotypic origin (Bos taurus or Bos indicus) ... more

S.C. Méo; C.R. Ferreira; M.R. Chiaratti; F.V. Meirelles; L.C.A. Regitano; M.M. Alencar; P.F. Barbosa
Conservation; Endangered species; Mitochondrial DNA; Oryx; Phylogenetic relationship

The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the topologies of respective ... more

H.A. Khan; I.A. Arif; A.A.Al Homaidan; A.H.Al Farhan
Mitochondrial DNA; Polymorphism; Sea turtles; Single strand conformation polymorphism

Marine turtles are increasingly being threatened worldwide by anthropogenic activities. Better understanding of their life cycle, behavior and population structure is imperative for the design of adequate conservation strategies. The mtDNA control region is a fast-evolving matrilineal marker that has been employed in the study of marine turtle populations. We developed and tested a simple ... more

E.C. Reis; R.M. Albano; A.C.V. Bondioli; L.S. Soares; G. Lôbo-Hajdu; G. Lôbo-Hajdu
A3243G mutation; Mitochondria; Mitochondrial disorders; Mitochondrial DNA; Transmitochondrial; Transmitochondrial mice

The mitochondrial A3243G tRNALeu(UUR) mutation associated with a variety of mitochondrial disorders results in a severe respiratory deficiency, an increase in reactive oxygen species (ROS) production and activities of anti-oxidative enzyme in vitro. However, the phenotypic implications of this mutation have not been described in vivo. Here, mitochondria carrying A3243G ... more

J. Li; K. Zhou; X. Meng; Q. Wu; S. Li; Y. Liu; J. Wang