Research Article

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08/30/2016
Bladder neoplasms; Dendritic cells; MAGE-3; Peptide; Vaccine

To determine the cytotoxic effect of lymphocytes activated by melanoma-associated antigen 3 (MAGE-3)-sensitized dendritic cells (DCs) on BIU-87 tumor cells, and to evaluate the possibility of MAGE-3-peptide-pulsed DCs as a vaccine in bladder cancer immunotherapy, the proliferation of T cells and the activity of cytotoxic T lymphocytes (CTLs) were examined by the MTT method. CTLs were ... more

X.Z. Li; Y. Han; J. Tian; X. Ren; X. Ma; M. Ma; X.Z. Li; Y. Han; J. Tian; X. Ren; X. Ma; M. Ma
03/18/2016
Genome sequencing; Genome-scale metabolic model; Lipase-producing strain; Systems biology

Lipase-producing bacteria are naturally-occurring, industrially-relevant microorganisms that produce lipases, which can be used to synthesize biodiesel from waste oils. The efficiency of lipase expression varies between various microbial strains. Therefore, strains that can produce lipases with high efficiency must be screened, and the conditions of lipase metabolism and optimization of ... more

N. Li; D.D. Li; Y.Z. Zhang; Y.Z. Yuan; H. Geng; L. Xiong; D.L. Liu; N. Li; D.D. Li; Y.Z. Zhang; Y.Z. Yuan; H. Geng; L. Xiong; D.L. Liu; N. Li; D.D. Li; Y.Z. Zhang; Y.Z. Yuan; H. Geng; L. Xiong; D.L. Liu
09/21/2015
Bayesian analysis; Phylogeny; Species group; Streptococcus

Alternative phylogenies for the genus Streptococcus have been proposed due to uncertainty about the among-species group relationships. Here, we performed a phylogenetic analysis of the genus Streptococcus, considering all the species groups and also the genomic data accumulated by other studies. Seventy-five species were subjected to a Bayesian phylogenetic analysis ... more

F. Póntigo; M. Moraga; S.V. Flores
08/21/2015
Biological evolution; Gossypium raimondii; Phylogeny; Sequence analysis; Synteny; TIFY gene family

The highly conserved TIFY domain is included in the TIFY protein family of transcription factors, which is important in plant development. Here, 28 TIFY family genes were identified in the Gossypium raimondii genome and classified into JAZ (15 genes), ZML (8), PPD (3), and TIFY (2). The normal (TIF[F/Y]XG) motif was dominant in the TIFY ... more

D.H. He; Z.P. Lei; B.S. Tang; H.Y. Xing; J.X. Zhao; Y.L. Jing
07/31/2015
morphology; Phylogeny; Salix matsudana; Species concept

The morphological species concept is based on morpho­logical traits, which are often subject to subjectivity or artifact. Molecular evidence is needed to test the reliability of morphological classification of taxa that are controversial and to provide appropriate taxonomic de­limitation. In this study, we used 15 single-copy nuclear loci and 2 chlo­roplast fragments to verify the ... more

S.H. Du; Z.S. Wang; Y.X. Li; D.S. Wang; J.G. Zhang
12/29/2015
Expression profile; GRAS; Jatropha curcas L.; Phylogeny; Physic nut

GRAS proteins play vital roles in plant growth and development. Physic nut (Jatropha curcas L.) was found to have a total of 48 GRAS family members (JcGRAS), 15 more than those found in Arabidopsis. The JcGRAS genes were divided into 12 subfamilies or 15 ancient monophyletic lineages based on the phylogenetic analysis of GRAS proteins from both flowering and ... more

Z.Y. Wu; P.Z. Wu; Y.P. Chen; M.R. Li; G.J. Wu; H.W. Jiang
12/29/2015
Microbiome; Mitis group; Phylogeny; RecN; Streptococcus massiliensis

Streptococcus is a diverse bacterial lineage. Species of this genus occupy a myriad of environments inside humans and other animals. Despite the elucidation of several of these habitats, many remain to be identified. Here, we explore a methodological approach to reveal unknown bacterial environments. Specifically, we inferred the phylogeny of the Mitis group by analyzing the ... more

F. Póntigo; C. Silva; M. Moraga; S.V. Flores
12/28/2015
Chinese catfish; Intraspecific variation; Mitochondrial genome; Phylogeny; Southern catfish; Structure

The complete mitochondrial genome of the Southern catfish (Silurus meridionalis) and the Chinese catfish (S. asotus), was determined using the long and accurate polymerase chain reaction (LA-PCR) method. The mitochondrial DNA nucleotide sequences of S. meridionalis and S. asotus were compared with those of 47 other catfish species in the same order. The ... more

Q.R. Wang; C. Xu; C.R. Xu; R.J. Wang
12/21/2015
Elymus species; Epichloë endophyte; Hordeum species; Host specificity; Maternal donor; Phylogeny

Epichloë endophytes are widely distributed mutualists of cool-season grasses and can protect their hosts against biotic and abiotic stresses. Previous studies have shown that Epichloë endophytes are specific to their grass hosts in tall Festuca and Lolium species. However, no systematic analysis exists of host specificity of asexual Epichloë ... more

H. Song; Q.Y. Song; X.Z. Li; Z.B. Nan

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