Research Article

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10/18/2013
marker-assisted selection; Psathyrostachys huashanica; RAPD; Repetitive sequence; SCAR; Wheat

In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that ... more

W.L. Du; J. Wang; L.M. Wang; Y.H. Pang; J. Wu; J.X. Zhao; Q.H. Yang; X.H. Chen
10/07/2013
MicroRNA; Northern blot; UV-B radiation; Wheat

MicroRNAs (miRNAs) play critical roles in post-transcriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the ... more

B. Wang; Y.F. Sun; N. Song; X.J. Wang; H. Feng; L.L. Huang; Z.S. Kang
05/25/2010
Genetic analysis; RAPD; Wild olives

Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP- ... more

M. Sesli; E.D. Yegenoglu
11/11/2013
DNA damage; Genetic analysis; Hair shaft; Mitochondrial DNA; Museum collection; Study skin

The condition of mtDNA in hair shafts preserved in a museum was examined using 30 study skins of masked palm civets, Paguma larvata (Viverridae), collected between 1924 and 2011. Comparisons of extracts from fresh and burnt alum-fixed hair shafts showed that burnt alum, which is commonly used in taxidermy, had no harmful effect on the amount of total DNA and lengths of the mtDNA ... more

N. Kurihara
04/02/2013
Equilibrium point; Genetic analysis; System dynamics model; Th1-Th2

In the human immune system, T-helper cells are able to differentiate into two lymphocyte subsets: Th1 and Th2. The intracellular signaling pathways of differentiation form a dynamic regulation network by secreting distinctive types of cytokines, while differentiation is regulated by two major gene loci: T-bet and GATA-3. We developed a system dynamics model to simulate the differentiation ... more

Q. Wang; M. Li; L.C. Xia; G. Wen; H. Zu; M. Gao
07/04/2014
Genetic analysis; Insect herbivory rates; Major gene plus polygene; Soybean

In order to investigate the genetic characteristics of soybean Leguminivora glycinivorella resistance and to improve soybean resistance insectivorous breeding efficiency by applying the multi-generation joint analysis method of the major gene plus polygene model, 5 pedigrees and generations (P1, F1, P2, F2, and F2:3) were used as the materials to perform the soybean L. ... more

J. Zhang; D. Yao; J. Ma; Y.P. Fu; J. Qu; P.W. Wang
05/11/2015
Dormancy; Genetic analysis; Lactuca sativa

Seed germination and dormancy are complex phenomena that are controlled by many genes and environmental factors. Such genes are indicated by phytohormones that interact with each other, and may cause dormancy or promote seed germination. The objective of this study was to investigate gene expression associated with the biosynthetic pathways of abscisic acid (ABA), gibberellic acid (GA), ... more

A.C.S. Clemente; R.M. Guimarães; D.C. Martins; L.A.A. Gomes; F. Caixeta; R.G.E. Reis; S.D.V.F. Rosa
04/30/2015
Body color; Development; Genetic analysis; Hybrid; Transparent crucian carp

The aim of this study was to investigate the genetic mechanism of the transparent trait in transparent crucian carp. We observed body color development in transparent crucian carp larvae and analyzed heredity of color in hybrids produced with red crucian carp, ornamental carp, and red purse carp. The results showed that the body color of the newly hatched larvae matured into the adult ... more

W. Xu; G.X. Tong; L.W. Geng; H.F. Jiang
07/18/2019
Abscisic acid; Coffea arabica; Gibberellic acid; qRT-PCR

During maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of ... more

F.Cdos Santos; S.D.V.F. Rosa; S.V.B. Coelho; A.C.S. Clemente; T.B. Fantazzini; C.C. Pereira; F. Caixeta; N.A.B. Fávaris; J.L. Baúte
06/22/2010
Conservation; MicroRNAs; Target genes; Wheat

MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation. A large number of miRNAs have been identified from model plant species; however, the character of conserved miRNAs is poorly understood. We studied 42 miRNA families that are conserved within the plant kingdom, using the miRBase database. Some conserved miRNA ... more

Z.J. Yin; F.F. Shen

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