Research Article

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02/25/2009
Allelic variability; Bombyx mori; Conformation-sensitive gel electrophoresis; Fibroin light chain; Silkworm

Conformation-sensitive gel electrophoresis is a useful method for identifying allele polymorphism; it provides co-dominant molecular markers. Using this method, we identified genetic variability in the third intron of the fibroin light chain gene, fib-L, in six Bombyx mori strains. Only Chinese C21A strain did not demonstrate allelic alterations, showing only homoduplex DNA ... more

J.F. Barbosa; J.P. Bravo; D.B. Zanatta; J.L.C. Silva; M.A. Fernandez
02/17/2009
Baculoviridae; Bombyx mori; infection process; Silk gland; Silkworm; trachea

A multiple nucleopolyhedrovirus previously isolated from infected Bombyx mori L. larvae (BmMNPV) in Paraná state, Brazil, was inoculated into B. mori larvae to examine susceptibility and cytopathology in silk gland cells. The anterior, middle and posterior silk glands were removed from the infected silkworm at different times post-inoculation and processed for cytopathology ... more

R.M.C. Brancalhão; E.F.B. Torquato; M.A. Fernandez
02/17/2009
Bombyx mori multiple nucleopolyhedrovirus; Molecular markers; Polymorphism; Random amplified polymorphic DNA; Silkworm

The silkworm Bombyx mori L. is particularly susceptible to virus diseases, especially B. mori nucleopolyhedrovirus (BmNPV). Disease resistance, along with high productivity, are important selection criteria for developing commercial hybrids of B. mori. We used bioassays and molecular markers linked to susceptibility/resistance to baculovirus infection to analyze the ... more

L.F.C. Ribeiro; D.B. Zanatta; J.P. Bravo; R.M.C. Brancalhão; M.A. Fernandez
07/19/2011
Chromosome 3; Linkage analysis; Oral lichen planus (OLP)

Oral lichen planus (OLP) is a common oral inflammatory disease affecting about 1-2% of the general adult population. As with European families who are diagnosed with OLP, the Chinese family who we studied was diagnosed with a severe form of oral reticular and erosive lesions; moreover, two of the five affected individuals developed oral cancer at an early age. A whole-genome genotyping ... more

Z. Wang; H. Yao; B. Cui; G. Ning; G.Y. Tang
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Linkage analysis; Microsatellites; Mystus nemurus; Pseudo-testcross strategy; River catfish; Segregation

The river catfish Mystus nemurus is an important fresh water species for aquaculture in Malaysia. We report the first genetic linkage map of M. nemurus based on segregation analysis and a linkage map using newly developed microsatellite markers of M. nemurus. A total of 70 of the newly developed polymorphic DNA microsatellite markers were analyzed on pedigrees ... more

B.P. Hoh; S.S. Siraj; S.G. Tan; K. Yusoff
12/19/2012
Genetic analyses; SSR markers; Transferability; “Cajuzinho-do-cerrado”

Use of molecular markers can be limited by the high cost and extensive time required for their development. Transfer of simple sequence repeat (SSR) markers reduces the cost and time limitations and has allowed the use of these markers in a larger number of species. We tested 11 SSR markers previously developed for Anacardium occidentale on A. humile. The 11 loci were ... more

L.G. Cota; P.A. Moreira; E.V. Menezes; A.S. Gomes; A.R.O. Ericsson; D.A. Oliveira; A.F. Melo
06/21/2011
Genetic diversity; Microsatellites; SSR markers; Wild sweet cherry

Wild sweet cherry (Prunus avium) trees are abundant in the northern part of Turkey, including the Coruh Valley. We analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper Coruh Valley in Turkey to determine genetic variation, using 10 SSR primers. These SSR primers generated 46 alleles; the number of alleles per primer ranged from 3 to 7, with a ... more

S. Ercisli; G. Agar; N. Yildirim; H. Karlidag; B. Duralija; A. Vokurka
03/09/2011
Curcuma longa L.; Genetic divergence; Genetic resources; Natural dye; SSR markers

Turmeric (Curcuma longa) is a triploid, vegetatively propagated crop introduced early during the colonization of Brazil. Turmeric rhizomes are ground into a powder used as a natural dye in the food industry, although recent research suggests a greater potential for the development of drugs and cosmetics. In Brazil, little is known about the genetic variability available for crop ... more

M.S. Sigrist; J.B. Pinheiro; J.A.Azevedo Filho; M.I. Zucchi
02/06/2013
Breeding; Genetic diversity; Phaseolus vulgaris; SSR markers

The identification of germplasm genetic variability in breeding programs of the common bean (Phaseolus vulgaris) is essential for determining the potential of each combination of parent plants to obtain superior genotypes. The present study aimed to estimate the extent of genetic diversity in 172 lines and cultivars of the common bean by integrating five tests of value for ... more

P.C.B. Cardoso; M.M. Veiga; I.P.P. de Menezes; P.A.M.R. Valdisser; T.C.O. Borba; L.C. Melo; M.J. Del Peloso; C. Brondani; R.P. Vianello
11/12/2012
Bulked segregant analysis; Drought tolerance; QTL; SSR markers; Wheat

This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under drought stress ... more

M.R.Naroui Rad; A. Kadir; M.Y. Rafii; H.Z.E. Jaafar; M.R. Naghavi

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