Research Article

Related GMR Articles

10/30/2013
Cathepsin L; Embryo development; Macrobrachium nipponense; Ovarian maturation; Yolk protein degradation

We identified the cDNA sequence of cathepsin L (CatL) in Macrobrachium nipponense, designated as MnCatL, for the first time. The MnCatL cDNA, isolated from the ovary, was 1710 bp in length, containing a 31-bp 5'-untranslated region, a 650-bp 3'-untranslated region, and an open reading frame of 1029 bp, encoding a 342-amino acid polypeptide with a predicted molecular mass of 37.7 ... more

W. Zhao; L. Chen; F. Zhang; P. Wu; E. Li; J. Qin
12/08/2009
Embryo development; Morpholino; RBM22; RNA-binding motif protein; Zebrafish

RBM22 is a newly discovered RNA-binding motif protein, belonging to the SLT11 family; it has been reported to be involved in pre-splicesome assembly and to interact with the Ca2+-signaling protein ALG-2. However, previous studies have not demonstrated whether its expression is essential for early embryogenesis in vertebrates. We utilized zebrafish as a developmental model to study the ... more

F. He; C.T. Wang; L.T. Gou
05/13/2013
Clone; Duck; PCR-RFLP; PLIN gene; SNP

The perilipin (PLIN) gene is a candidate gene of carcass and fat traits in ducks. In order to study the molecular character of the PLIN gene and its function in different breeds of Chinese ducks, samples were obtained from the Chinese Academy of Agricultural Sciences Research Center for Birds, including 95 Peking ducks of the Z2 series, 91 Peking ducks of the Z4 series, ... more

H.L. Zhang; H.J. Fan; X.L. Liu; Y. Wu; S.S. Hou
12/29/2015
Duck; Plumage color; Promoter; SNP; Tyrosinase gene

Tyrosinase, encoded by the TYR gene, is the rate-limiting enzyme in the production of melanin pigment. In this study, plumage color separation was observed in Cherry Valley duck line D and F1 and F2 hybrid generations of Liancheng white ducks. Gene sequencing and bioinformatic analysis were applied to the 5'-regulatory region of TYR, to explore the connection between ... more

N.N. Zhang; J.W. Hu; H.H. Liu; H.Y. Xu; H. He; L. Li
12/17/2015
Differentially expressed genes; Duck; LPS; Spleen

Lipopolysaccharide (LPS), the major component of the outer cell wall of Gram-negative bacteria, activates the immune system and threatens the health of livestock and poultry. However, little is known about the genes and pathways involved in the immune response of ducklings to LPS. To elucidate the genes involved in the response of 7-day-old duckling spleens treated with LPS, RNA from LPS- ... more

B. Deng; T. Yu; W. Liu; S.Q. Ye; L.X. Wang; Y. Yang; P. Gong; Z.P. Ran; H.J. Huang; J.H. Wen
08/30/2016
Duck; Fatty acid composition; SCD1; Serum biochemical levels; SNPs

Stearoyl-coenzyme A desaturase 1 (SCD1) is the key limiting enzyme in the synthesis of monounsaturated fatty acids, and plays a crucial role in the regulation of oleic acid. In this study, 165 ten-week-old Cherry Valley ducks were used to investigate single nucleotide polymorphisms (SNPs) in the 5' regulatory region of the SCD1 gene, and their associations with duck serum ... more

W.G. Li; Y.Y. Zhang; D.D. Wang; W.G. Li; Y.Y. Zhang; D.D. Wang
05/15/2012
NANOG; Neural differentiation; NT2/D1; OCT4; Proliferation; SOX2

SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and ... more

D. Drakulic; A. Krstic; M. Stevanovic
04/19/2012
Amniotic fluid; Cryopreservation; Differentiation; NANOG; SOX2; Stem cell

Cryopreservation of mesenchymal stem cells from amniotic fluid is of clinical importance, as these cells can be harvested during the prenatal period and stored for use in treatments. We examined the behavior of mesenchymal stem cells from human amniotic fluid in culture that had been subjected to cryopreservation. We assessed chromosomal stability through karyotype analysis, determined ... more

P.C. Ângelo; A.C.S. Ferreira; V.D. Fonseca; S.P. Frade; C.S. Ferreira; F.S.V. Malta; A.K. Pereira; H.V. Leite; A.P. Brum; V.C. Pardini; K.B. Gomes; A.C.V. Cabral