Research Article

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Brachiaria; Breeding; Forage grass; Hybridization; Meiosis

Brachiaria humidicola is a grass adapted to seasonally swampy grasslands in Africa; two cultivars, ‘common’ and Llanero, are widely used in Brazilian pastures. New cultivars are in great demand in order to diversify current production systems to achieve improved quality and yield. Cytological analyses of 55 accessions of this species available from the Embrapa Beef Cattle ... more

K.R. Boldrini; E.V. Adamowski; N. Silva; M.S. Pagliarini; C.B. Valle
Elephant grass; Flow cytometry; Grasses; Hybridization

Interspecific hybridization between Napier grass (Pennisetum purpureum), which is widely grown in Brazil for cattle forage, and pearl millet (Pennisetum glaucum) has been used as a breeding strategy for the development of improved cultivars. However, the hybrid between these two species is sterile due to its triploid condition (2n = 3x = 21 chromosomes), which hinders ... more

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Adventitious embryony; Aneuploidy; Apospory; Hybridization; Polyploidy; Sterility

Cassava is the most important staple crop in the Tropics and Subtropics. Apomixis may revolutionize its production due to various attributes. These potential advantages include production by true seed, maintaining cultivar superiority over generations without segregation, and avoiding contamination by bacteria and viruses. Historically, apomixis was initially observed by International ... more

D.Y.H. Freitas; N.M.A. Nassar
Growth regulators; Hybridization; In vitro culture; Micro-propagation; Multiple shooting; Seed culture

In breeding programs, a large number of F2 individuals are required to perform the selection process properly, but often few such plants are available. In order to obtain more F2 seeds, it is necessary to multiply the F1 plants. We developed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of seeds using 6- ... more

M.A. Espósito; P. Almirón; I. Gatti; V.P. Cravero; F.S.L. Anido; E.L. Cointry
genetic breeding; Hybridization; Inbreeding; Molecular marker; Saccharum spp; Self-fertilization

Superior inbred clones selected in S1 families can integrate an individual reciprocal recurrent selection program in sugarcane by eliminating the genetic load of the population and exploring superior hybrid combinations. Molecular markers can be used for reliable identification of the true selfing-derived clones in these S1 populations. The objective of this study ... more

P.M.A. Costa; C.F. Almeida; G. Silveira; B. Soares; D.C.F. Baffa; L.A. Peternelli; L.L. Bhering; M.H.P. Barbosa
Conservation; Hybridization; SSR molecular marker; Wild cotton

Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridization with exotic species of ... more

I.P.P. de Menezes; J.O. da Silva; G. Malafaia; R.D.D. Silveira; P.A.V. Barroso
DNA extraction; DNA isolation kits; Grapevine

Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols and three commercial DNA ... more

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Expressed sequence tag (EST)-derived simple sequence repeat (SSR); Grapevine; non-EST SSR; Polymorphism

Among different classes of molecular markers, expressed sequence tags (ESTs) are a new resource for developing simple sequence repeat (SSR) functional markers for genotyping and genetic mapping in F1 hybrid populations of Vitis vinifera L. Recently, because of the availability of an enormous amount of data for ESTs in the public domain, the emphasis has shifted from ... more

E. Kayesh; N. Bilkish; G.S. Liu; W. Chen; X.P. Leng; J.G. Fang
Grape weight; Grapevine; QTL; Soluble solids

A grapevine hybrid population was derived from a crossing of the early-maturing female parent cultivar ‘87-1’ and the late-maturing male parent cultivar ‘9-22’. A total of 149 plants were selected from the hybrid population as the mapping population, and after sequence-related amplified polymorphism and simple-sequence repeat marker analysis were conducted we constructed molecular ... more

Y.H. Zhao; Y.S. Guo; H. Lin; Z.D. Liu; H.F. Ma; X.W. Guo1; K. Li; X.X. Yang; Z.Z. Niu; G.G. Shi