Research Article

Related GMR Articles

07/12/2011
Bov-A2; Cattle; CYP21 gene; Epigenetics; Methylation; SINE

The CYP21 (steroid 21-hydroxylase) gene is involved in the synthesis of steroid hormones. Bov-A2 is a retroposon that is common in ruminant genomes. The promoter region of bovine CYP21 contains a short interspersed nucleotide element of Bov-A2, which overlaps a putative Sp1 binding site. We looked for RFLP/HpaII polymorphism in the Bov-A2 element in bovine Zebu ... more

A.M. da Silva; M.A.R. de Freitas; A.F.L. Rios; A. Renzi; R.B. Lôbo; M.A.V. Galerani; R.A. Vila; E.S. Ramos
01/27/2009
Cattle; Epigenetics; Genomic imprinting; IGF2; IGF2R; Nuclear transfer

Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by ... more

F. Perecin; S.C. Méo; W. Yamazaki; C.R. Ferreira; G.K.F. Merighe; F.V. Meirelles; J.M. Garcia
04/10/2013
Colorectal cancer; DNA hypomethylation; Epigenetics; Gene expression; Metastasis

DNA methylation is a fundamental epigenetic mechanism in regulating the expression of genes controlling crucial cell functions in cancer development. Gene silencing via CpG island methylation/demethylation in the promoter region is one of the mechanisms by which different genes are inactivated/activated in human cancers. Tissue inhibitor of metalloproteinase-2 (TIMP-2) is known to ... more

Z. Hussain; M.I. Khan; M. Shahid; F.N. Almajhdi
12/09/2005
Androgen receptor; Methylation; Neonatal screening; Turner syndrome

Turner syndrome (TS) is one of the most common human chromosomal abnormalities; it is characterized by the presence of one normal X chromosome and the complete or partial loss of the second X chromosome. The early recognition of TS patients allows for adequate therapy for short stature and pubertal sex steroid substitution. We developed a cost-effective molecular diagnostic tool that ... more

M.Neves Rocha; M.Rezende Melo; C.Alberto Longui; D.Vilariço de Oliveira; C.Costa Figueiredo; P.Roberto Pacchi
01/05/2009
meningiomas; metastases; Methylation; Polymorphism; schwannomas; TP53

The p53 tumor suppressor gene is the most frequently mutated gene in human cancer; this gene is mutated in up to 50% of human tumors. It has a critical role in the cell cycle, apoptosis and cell senescence, and it participates in many crucial physiological and pathological processes. Polymorphisms of p53 have been suggested to be associated with genetically determined susceptibility in various ... more

L.O. Almeida; A.C. Custódio; G.R. Pinto; M.J. Santos; J.R.W. Almeida; C.A. Clara; J.A. Rey; C. Casartelli
11/12/2012
hMLH1; Meningioma; Methylation; Microsatellite instability

We investigated a possible role of hMLH1 hypermethylation and microsatellite instability in meningioma progression. Fifty meningomas were examined for methylation of hMLH1 using a methylation-specific PCR; 43 of them were analyzed for microsatellite instability using nine microsatellite markers. Loss of heterozygosity on chromosome 22q was detected using two markers. Two atypical ... more

M.N. Chen; P. Wang; J. Zhang; B.Y. Zhou; Q. Mao; Y.H. Liu
05/07/2012
Intron variation; Methylation; NR gene; Sibling cultivars; Tobacco

To better understand genomic DNA methylation in sibling plant cultivars, methylation-sensitive amplification polymorphism analysis was used to investigate two sibling tobacco cultivars, Yunyan85 and Yunyan87, and their two parents, K326 and Yunyan No. 2. Differences in the degree of genomic DNA methylation were found among the four tobacco cultivars. Compared with parents, the two ... more

S.L. Fu; Z.X. Tang; L. Liu; L.M. Lu; Y.B. Huang
02/13/2014
Epithelial-mesenchymal transition; Lung epithelial cells; Methylation; TGF-β; Transforming growth factor

The aim of this study was to investigate the specific molecular mechanism of the transforming growth factor β (TGF-β)-induced epithelial-mesenchymal transition in a lung cancer cell line, and to provide new ideas for targeting therapy of lung cancer. A549 cells were treated with different concentrations of TGF-β and 5-aza-deoxycytidine (5-aza-dC). The morphological changes after the ... more

Y.Y. Liu; J.Y. Han; S.C. Lin; Z.Y. Liu; W.T. Jiang
07/07/2014
Asian; Hepatocellular carcinoma; Meta-analysis; Methylation; RUNX3

Runt-related transcription factor 3 (RUNX3) is a potential tumor suppressor that is frequently hypermethylated in hepatocellular carcinoma (HCC). The present meta-analysis of case-control studies was carried out to determine whether RUNX3 hypermethylation is associated with HCC. The PubMed, Embase, and Chinese National Knowledge Infrastructure databases were searched for ... more

X.X. Lu; L.Q. Zhu; F. Pang; W. Sun; C. Ou; Y. Li; J. Cao; Y.L. Hu
01/17/2014
Chinese population; Colorectal cancer; Methylation; SEPT9

Methylation of the septin 9 gene (SEPT9) occurs in higher frequency in colorectal cancer (CRC) compared to control samples, which suggests that SEPT9 methylation is a useful biomarker for screening CRC. However, the methylation status of SEPT9 in Chinese CRC patients is scarcely reported. In the present study, SEPT9 methylation was tested in CRC tissues ... more

X.L. Su; Y.F. Wang; S.J. Li; F. Zhang; H.W. Cui

Pages