Methodology

Related GMR Articles

12/18/2009
Aplastodiscus; Hypsiboas; Karyotype; Nucleolus organizer region

Four species of Aplastodiscus and two species of Hypsiboas were cytogenetically compared. Aplastodiscus perviridis, A. cochranae, H. albomarginaus, and H. faber had 2n = 24 chromosomes, while A. albosignatus and A. leucopygius had 2n = 20 and 2n = 18 chromosomes, respectively. Aplastodiscus perviridis and A ... more

K.A. Carvalho; P.C.A. Garcia; S.M. Recco-Pimentel
08/24/2010
Karyotype; Marker chromosome; Small supernumerary marker chromosomes; Spontaneous abortion

We report a phenotypically normal couple with repeated spontaneous abortions and without other clinical features. Clinical, hematological, biochemical, and endocrinological aspects of the couple did not reveal any abnormalities. The karyotype of the wife was normal (46,XX), while the husband was found to have an abnormal karyotype, 47,XY,+der(22)mat. The marker chromosome was familial and non- ... more

M. Balkan; H. İsi; H. İsi; A. Gedik; M. Erdemoğlu; T. Budak
03/02/2010
Heterochromatin; Karyotype; Non-disjunction; Rhamdia; Supernumerary chromosomes

Among catfish species of the genus Rhamdia reported for the Brazilian territory, R. quelen is the most widespread, being found in nearly all hydrographic basins of Brazil. Nowadays, R. quelen is a synonym for at least 47 other species in this genus, its taxonomic status still being controversial. The available cytogenetic reports show a wide variation in the ... more

C. Garcia; C. Oliveira; L.F. Almeida-Toledo
09/19/2013
Chromosome evolution; Diploid number; Heterophrynus longicornis; Karyotype; Sex chromosome system

We analyzed mitotic and meiotic cells of a Brazilian amblypygid, Heterophrynus longicornis, using conventional and molecular cytogenetic techniques (Giemsa staining, C-banding, Ag-NOR, and FISH with rDNA probe). This is the first study that focuses solely on amblypygid chromosomes; it was undertaken to add data on cytogenetic knowledge of this group and contribute to the ... more

E. Paula-Neto; D. Araujo; L.S. Carvalho; D.M. Cella; M.C. Schneider
08/29/2013
Ag-NORs; Capra hircus; Chromosomal banding; Karyotype

In order to provide the first cytogenetic data of naturalized and threatened goat breeds from northeastern Brazil, cytogenetic analyses were carried out in individuals of Repartida and Moxotó breeds raised in Bahia and Ceará States. Males and females of both breeds had 2n = 60, with 29 autosomal acrocentric pairs plus the sex chromosome pair. The number of nucleolar organizer region (NOR ... more

R.P. Santos; P.R.A.M. Affonso; D. Diniz; A.S. Medrado; K.M. Silva; P.L.S. Carneiro
07/08/2013
AZF microdeletions; Azoospermia; Glutamine and glycine repeat polymorphisms; Karyotype; Non-obstructive oligospermia

Chromosome abnormalities, Y-chromosome microde­letions, and androgen receptor gene CAG and GGN repeat polymor­phisms in infertile Chinese men featuring severe oligospermia and azoospermia were analyzed. Ninety-six fertile men and 189 non-ob­structive infertile men, including 125 patients with azoospermia and 64 with severe oligozoospermia, were studied. Seventeen infertile men (9.0%) ... more

T.T. Han; J. Ran; X.P. Ding; L.J. Li; L.Y. Zhang; Y.P. Zhang; S.S. Nie; L. Chen
04/25/2013
45S rDNA; FISH; Karyotype; NOR; Silver staining; Smilax

The genus Smilax (Smilacaceae) includes species of medicinal interest; consequently, their identification is important for the control of raw material used in the manufacture of phytotherapeutic products. We investigated the karyotype of Smilax rufescens in order to look for patterns that would be useful for comparative studies of this genus. To accomplish this, we ... more

D. Pizzaia; V.M. Oliveira; A.R. Martins; B. Appezzato-da-Glória; E. Forni-Martins; M.L.R. Aguiar-Perecin
08/16/2012
Beetle; Fluorochrome; Heterochromatin; Karyotype; NOR

Males of Zophobas aff. confusus and Nyctobates gigas (Tenebrionidae) collected in the State of Pernambuco, Brazil, were studied through conventional staining, C-banding, silver nitrate impregnation (AgNO3), and the base specific fluorochromes CMA3 and DAPI. Z. aff. confusus was found to have 2n = 20 (9+Xyp) while N. ... more

A.C. Lira-Neto; G.M. Silva; R.C. Moura; M.J. Souza
01/19/2012
Brahman cow (Bos indicus); Hybrids of mithun x Brahman; Karyotype; Mithun (Bos frontalis); Rob(2;28)

We examined the cytogenetics of mithun (Bos frontalis), a domesticated version of the Asian gaur, and hybrids (F1 generation) produced by artificial insemination of Brahman cows (Bos indicus) with mithun semen. Reproductive potential was also examined in the F1 generation and a backcrossed heifer for utilization of heterosis. Metaphase chromosome ... more

K.X. Qu; Z.X. He; W.H. Nie; J.C. Zhang; X.D. Jin; G.R. Yang; X.P. Yuan; B.Z. Huang; Y.P. Zhang; L.S. Zan
10/31/2014
Chromosome number; Karyotype; Rheum palmatum

Rheum palmatum, one of the source plants of the traditional Chinese medicine rhubarb, is anendemic and endangered species. To our knowledge, this is the first report on the chromosome number and karyotype of this species. Sectioning combined with micrography was used to analyze the karyotype. The following results were obtained: R. palmatum had a stable chromosome ... more

J.Q. Ye; Y.Y. Jia; K. Fan; X.J. Sun; X.M. Wang

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