Research Article

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Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out ... more

C.M. Han; R. Chen; T. Li; X.L. Chen; Y.F. Zheng; M.T. Ma; Q.H. Gao; C.M. Han; R. Chen; T. Li; X.L. Chen; Y.F. Zheng; M.T. Ma; Q.H. Gao
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3T3-L1 preadipocyte; Adipocyte differentiation; AP-1; FABP4; MicroRNA

We examined the effect of microRNAs on 3T3-L1 adipocyte differentiation and expression of adipocyte-specific gene fatty acid-binding protein 4 (FABP4). We screened and identified adipo-related microRNAs during 3T3-L1 adipocyte differentiation with a microRNA microarray. High expression plasmids of miR-24 and miR-21 were constructed and transfected into 3T3-L1 preadipocytes by ... more

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01/16/2015
FABP4; Genotyping; PPP3CA; SNPs

The objective of this study was to identify associations between known polymorphisms in genes related to adipose tissue and sexual precocity in Nellore cattle. A total of 1689 precocious and non-precocious heifers belonging to farms participating in Conexão Delta G breeding program were studied. SNPs from the Illumina High-Density Bovine SNP BeadChip were used. This chip contains 777,000 ... more

M.M. Dias; F.R.P. Souza; L. Takada; F.L.B. Feitosa; R.B. Costa; I.D.P.S. Diaz; D.F. Cardoso; R.L. Tonussi; F. Baldi; L.G. Albuquerque; H.N. Oliveira
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Adipocyte; Chicken; Leptin receptor; Lipid metabolism; RNA interference

In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, and lipoprotein ... more

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Apoptosis; Gene and protein expression; MGC; shRNA; siRNA; VEGF

Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonucleotides targeting the ... more

N. Ally; X.L. Zou; B.C. Jiang; L. Qin; L. Zhai; P. Xiao; H.L. Liu
04/13/2015
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Hypoxia reduces the oxygen supply to tumor cells and may limit tumor cell growth. However, hypoxia promotes tumor cell metabolic adaptation, apoptosis resistance, angiogenesis, invasion, and metastasis. Hypoxia-inducible factor-2α (HIF-2α) may be responsible for these hypoxia-induced changes. In this study, we investigated the effects of hypoxia and HIF-2α knockdown in HeLa cells. HIF-2α ... more

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