Research Article

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We described five novel microsatellite loci for the piracema fish species Prochilodus lineatus (Characiformes), endemic to South America and of extreme importance to both commercial and artisanal fisheries across its occurrence area. A primary, unenriched genomic library was constructed and radioactively screened for repetitive motifs. Positive clones were automatically ... more

G.M. Yazbeck; E. Kalapothakis
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Chromatin; DNA methylation; FISH; Micrococcal nuclease; Myostatin

Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in several tissues (liver, eye, kidney, brain ... more

E.M. Abbas; A. Takayanagi; N. Shimizu; M. Kato
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FISH; Genetic conservation; RAPD; Surubim

Steindachneridion melanodermatum is a large Brazilian catfish, highly prized for sport fishing and for its meat. Specimens of this species, both caught in nature from Iguacu River and F1 fish born in captivity, were analyzed with regard to patterns of RAPD molecular markers. Genetic similarity ranged from 0.57 to 0.95; two groups were determined for the wild specimens ... more

D.A. Matoso; M. da Silva; M.C.S. Cortinhas; M.M. Cestari; M.C. de Almeida; M.R. Vicari; R.F. Artoni
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C.E.S. Zago
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Breast cancer; Chromosome 17; FISH; Metastasis; TP53 gene

Breast cancer is the second most common origin of brain metastases, after lung cancer, and represents 14-20% of all cases. Abnormalities of chromosome 17 are important molecular genetic events in human breast cancer, and several oncogenes and tumor suppressor genes are located on this chromosome. In about half of all human cancers, the tumor suppressor gene TP53, located at 17p13 ... more

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The genus Smilax (Smilacaceae) includes species of medicinal interest; consequently, their identification is important for the control of raw material used in the manufacture of phytotherapeutic products. We investigated the karyotype of Smilax rufescens in order to look for patterns that would be useful for comparative studies of this genus. To accomplish this, we ... more

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Evolution; FISH; Neotropical region; Systematic phylogeny

A species complex hypothesis involving Astyanax fasciatus from southern Brazil was tested using 12S mtDNA sequences. Phylogenetic inferences were performed with maximum likelihood, maximum parsimony and Bayesian as phylogenetic methods and Hemigrammus bleheri as the outgroup. Besides 11 sequences from A. fasciatus, the data set was comprised of other partial ... more

D.A. Matoso; M. da Silva; R.F. Artoni; R.A. Torres
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Chromosome polymorphism; Cytogenetics; FISH; Histone gene; rDNA

We sampled 11 natural populations of the grasshopper Xyleus discoideus angulatus in Northeastern Brazil to analyze B chromosome frequency and meiotic behavior. We observed a single large B chromosome, resembling the X chromosome, in 29 of the 402 specimens. Eight of the 11 populations had B chromosomes, with a rather broad geographical distribution, suggesting that this is an ... more

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Cebus apella; Chromosome evolution; Heterochromatin; Micro-FISH

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M. Nieves; M. Mühlmann; M. Mühlmann; M.Dolores Mudry
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Blastocerus; Cervidae; Chromosome evolution; Chromosome fragility; Mazama

The tendency toward chromosome fragility is one of the theories that may explain chromosome variation in brocket deer species (genus Mazama). We tested doxorubicin as an inducer of chromosome aberrations in lymphocytes of three brocket deer species, Mazama gouazoubira, M. americana and M. nana, compared to the marsh deer, Blastocerus dichotomus ... more

D.S.F. Vargas-Munar; J.A. Sarria-Perea; J.M.B. Duarte

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