Methodology

Related GMR Articles

10/06/2011
ISSR; Molecular marker; RAPD

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 ... more

D.T. Phong; V.T.T. Hien; T.T.V. Thanh; D.V. Tang
09/16/2011
Genetic variation; Morphometric; Multivariate analysis; RAPD; Rastrelliger spp

Mackerel (Scombridae; Rastrelliger) are small commercially important pelagic fish found in tropical regions. They serve as a cheap source of animal protein and are commonly used as live bait. By using a truss morphometrics protocol and RAPD analysis, we examined morphological and genetic variation among 77 individual mackerel that were caught using long lines and gillnets at 11 ... more

M.N. Darlina; A.R. Masazurah; P. Jayasankar; A.F.J. Jamsari; A.M.N. Siti
07/19/2011
Efficacy; Genetic variation; Novel bands; Primer-pair arrays; RAPD

The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intra- and inter-specific levels. Amplification ... more

E.M. Gatphoh; S.K. Sharma; K. Rajkumari; R. Rao
10/17/2013
Genetic variability; ISSR; Mandacaru; morphology; RAPD

This is the first study to examine the genetic diversity of mandacaru cactus (Cereus jamacaru P. DC.). Plants of spineless mandacaru are commonly found in gardens and parks of urban areas in northeastern Brazil. In addition to exploring their ornamental potential, morphological, and genetic characterization may contribute to the development of plant materials that can be used as ... more

F.I.C. Oliveira; P.N. Bordallo; A.C.R. Castro; D. Correia
08/12/2013
Fusarium verticillioides; Intron; ISSR; RAPD

Fusarium verticillioides is a pathogen of agriculturally im­portant crops, especially maize. It is considered one of the most impor­tant pathogens responsible for fumonisin contamination of food products, which causes severe, chronic, and acute intoxication in humans and ani­mals. Moreover, it is recognized as a cause of localized infections in im­munocompetent patients and ... more

S.C. Chang; D.P.C. Macêdo; C.M. Souza-Motta; N.T. Oliveira
03/13/2013
Interspecific hybrids; ISSR; Morphological trait; RAPD; Solanum melongena; Solanum torvum

Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed ... more

J. Kumchai; Y.C. Wei; C.Y. Lee; F.C. Chen; S.W. Chin
08/27/2012
Achillea species; Genetic diversity; ISSR; Marker; RAPD

Cultivation and preservation of yarrow has recently attracted wide attention due to its beneficial properties; however, genetic variation of Achillea species is still relatively unknown. We used RAPD and ISSR markers to assess genetic diversity in 16 accessions of yarrow belonging to two species native to Iran. Seven ISSR and nine RAPD primers generated 187 amplified fragments, ... more

M. Ebrahimi; M. Farajpour; M. Rahimmalek
06/29/2012
ISSR; RAPD; Sequence characterized amplification region; Thinopyrum elongatum

Diploid Thinopyrum elongatum, a wild relative of wheat, contains many agronomically desirable traits and has potential for increasing genetic variability and introducing desirable characters in this crop. Few molecular markers are available for rapid screening of T. elongatum genome segments in the wheat genetic background. We used 36 RAPD primers and 33 ISSR primers to ... more

G.H. Xu; W.Y. Su; Y.J. Shu; W.W. Cong; L. Wu; C.H. Guo
08/26/2011
RAPD; Red rot; SSR; Sugarcane; Variability

Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog ... more

M.T.H. Shahid; F.A. Khan; A. Saeed; I. Fareed
08/10/2010
Eggplant; Genetic resources; RAPD; Solanum melongena; SSR

Eggplant is a major crop in Turkey, which produces more of this crop than all of Europe; consequently, germplasm resources are of concern for the country. Molecular characterization of eggplant genotypes collected from different geographical regions of Turkey was carried out using SSR and RAPD markers. With amplification of five SSR loci, the number of alleles per microsatellite locus ... more

K. Demir; M. Bakır; M. Bakır; G. Sarıkamış; S. Acunalp

Pages