Research Article

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12/08/2011
Chromatin; DNA methylation; FISH; Micrococcal nuclease; Myostatin

Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in several tissues (liver, eye, kidney, brain ... more

E.M. Abbas; A. Takayanagi; N. Shimizu; M. Kato
10/25/2011
FISH; Genetic conservation; RAPD; Surubim

Steindachneridion melanodermatum is a large Brazilian catfish, highly prized for sport fishing and for its meat. Specimens of this species, both caught in nature from Iguacu River and F1 fish born in captivity, were analyzed with regard to patterns of RAPD molecular markers. Genetic similarity ranged from 0.57 to 0.95; two groups were determined for the wild specimens ... more

D.A. Matoso; M. da Silva; M.C.S. Cortinhas; M.M. Cestari; M.C. de Almeida; M.R. Vicari; R.F. Artoni
07/19/2011
C-banding; FISH; G-banding; Karyotype; Nucleolar organizer regions; Turtle
C.E.S. Zago
01/04/2013
Breast cancer; Chromosome 17; FISH; Metastasis; TP53 gene

Breast cancer is the second most common origin of brain metastases, after lung cancer, and represents 14-20% of all cases. Abnormalities of chromosome 17 are important molecular genetic events in human breast cancer, and several oncogenes and tumor suppressor genes are located on this chromosome. In about half of all human cancers, the tumor suppressor gene TP53, located at 17p13 ... more

D.S. Vasconcelos; F.P.E. da Silva; L.G. Quintana; N.P. Anselmo; M.A.K. Othman; T. Liehr; E.H.C. de Oliveira
04/25/2013
45S rDNA; FISH; Karyotype; NOR; Silver staining; Smilax

The genus Smilax (Smilacaceae) includes species of medicinal interest; consequently, their identification is important for the control of raw material used in the manufacture of phytotherapeutic products. We investigated the karyotype of Smilax rufescens in order to look for patterns that would be useful for comparative studies of this genus. To accomplish this, we ... more

D. Pizzaia; V.M. Oliveira; A.R. Martins; B. Appezzato-da-Glória; E. Forni-Martins; M.L.R. Aguiar-Perecin
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Chromosome evolution; Fiber-FISH; FISH; Hypostominae

We examined chromosomes of three species of the genus Hypostomus, in order to contribute to the understanding of the karyotype evolution of this group. Specimens of H. ancistroides and H. nigromaculatus displayed differences in karyotype formulas, distribution and location of heterochromatin and nucleolus organizer regions when compared to other populations of ... more

J.B. Traldi; D.R. Blanco; M.R. Vicari; J.F. Martinez; R.L. Lui; A.V. Barros; R.F. Artoni; O. Moreira-Filho
03/07/2013
Evolution; FISH; Neotropical region; Systematic phylogeny

A species complex hypothesis involving Astyanax fasciatus from southern Brazil was tested using 12S mtDNA sequences. Phylogenetic inferences were performed with maximum likelihood, maximum parsimony and Bayesian as phylogenetic methods and Hemigrammus bleheri as the outgroup. Besides 11 sequences from A. fasciatus, the data set was comprised of other partial ... more

D.A. Matoso; M. da Silva; R.F. Artoni; R.A. Torres
03/26/2014
Chromosome polymorphism; Cytogenetics; FISH; Histone gene; rDNA

We sampled 11 natural populations of the grasshopper Xyleus discoideus angulatus in Northeastern Brazil to analyze B chromosome frequency and meiotic behavior. We observed a single large B chromosome, resembling the X chromosome, in 29 of the 402 specimens. Eight of the 11 populations had B chromosomes, with a rather broad geographical distribution, suggesting that this is an ... more

C.B. Machado; L.C.Silva Neto; V. Loreto; M.J. Souza
09/30/2003
Bovine; Comparative map; FISH; Gene assignment

Seven genes were assigned by molecular cytogenetic methods to bovine chromosome 5. To accomplish this, specific primers were either publicly available or were designed from highly conserved regions of the publicly available mammalian gene sequences. The identity of the amplified segments was verified by sequencing and alignment with the published sequences. The optimized primers that amplified ... more

M. De Donato; D.S. Gallagher; C. Lehn; C. Gill; J.F. Taylor
12/08/2011
Chromosome banding; FISH; Karyotype variability; Topotypes

A large number of genera in the tropical fish family Characidae are incertae sedis. Cytogenetic analysis was made of four of these species: Astyanax eigenmanniorum, Deuterodon stigmaturus, Hyphessobrycon luetkenii, and H. anisitsi, collected from various hydrographic basins: hydrographic system from Laguna dos Patos/RS, Tramandaí basin/RS and ... more

M.M. Mendes; R. da Rosa; L. Giuliano-Caetano; A.L. Dias

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