Research Article

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08/06/2012
Cultivar identification; Molecular markers; RAPD; Sweet orange

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar ... more

X. Sun; Q. Mu; D. Jiang; C. Wang; X.C. Wang; J.G. Fang
05/07/2012
Cultivar identification; Molecular markers; Peach; RAPD

DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11-mer primers were used to amplify the genotypes; all 95 peach ... more

M.L. Yu; W.Y. Wang; R.J. Ma; Z.J. Shen; J.G. Fang
01/08/2014
Cultivar identification; Molecular markers; Ornamental peach; RAPD

One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram strategy for efficient identification of plant cultivars, from ... more

J. Han; W.Y. Wang; X.P. Leng; L. Guo; M.L. Yu; W.B. Jiang; R.J. Ma
06/01/2015
Cultivar identification; Genetic diversity; Genetic similarity; ISSR; Olea europaea L.; SSR

The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples ... more

M.M. Zhan; Z.Z. Cheng; G.C. Su; A.Y. Wang; H.P. Chen; Z.S. Yang; Z. Shan; Q.M. Huang
01/25/2008
Dialell; Heterotic groups; Molecular markers; SSR

The objective of the present study was to correlate the genetic distances (GD) of single cross hybrids with yield, heterosis and specific combining ability (SCA) in the double cross hybrid synthesis. For this, 10 single cross commercial hybrids were used from different companies, and all the possible double hybrids were synthesized by a complete dialell. The hybrids were assessed in 15 ... more

M. Balestre; J.C. Machado; J.L. Lima; J.C. Souza; N. Filho
05/24/2011
Cultivar identification; Molecular markers; Pear; RAPD

Accurate and reliable cultivar identification of crop species is essential to guarantee plant material identity for purposes of registration, cultivar protection and production. To facilitate identification of plant cultivars, we developed a novel strategy for efficient recording of DNA molecular fingerprints in genotyped plant individuals. These fingerprints can be used as efficient ... more

J. Lin; X.C. Wang; Y.H. Chang; J.G. Fang
09/21/2010
DNA fingerprinting; Molecular markers; Olea europaea; SSR

Olea europaea is one of the oldest species of domesticated trees. We used microsatellite markers for fingerprinting and for evaluation of genetic similarity and structure of 26 Greek olive cultivars, which cover most of the olive cultivation regions of Greece, including previously undescribed denominations from northern Greece. Eighty-one alleles were revealed with six SSR loci ... more

K. Roubos; M. Moustakas; F.A. Aravanopoulos
04/14/2014
Molecular markers; SSR; Transferability; Tuber crop; Yams

Dioscorea cayenensis and Dioscorea rotundata are among the most important yam species for the humid and sub-humid tropics. We isolated nine polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. The nine primer pairs were validated in 22 D. cayenensis accessions, and were tested for transferability in 26 D. rotundata ... more

L.R.G. Silva; M.M. Bajay; M. Monteiro; T.F. Mezette; W.F. Nascimento; M.I. Zucchi; J.B. Pinheiro; E.A. Veasey
01/24/2013
Cultivar identification; Ginkgo biloba; RAPD markers

Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagram that ... more

G.P. Li; C.Q. Zhang; F.L. Cao
07/29/2014
Cultivar identification; Genetic relationship; Inter-simple sequence repeat; Lily

Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average of 13 bands were amplified by each primer. Two software packages, POPGENE 1.32 and NTSYSpc 2.1, were used to analyze the data matrix. Our results showed that the observed number of ... more

G.F. Cui; L.F. Wu; X.N. Wang; W.J. Jia; Q. Duan; L.L. Ma; Y.L. Jiang; J.H. Wang

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