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Related GMR Articles

09/12/2013
Cerrado; Conservation; Genetic variability; Germplasm; Multivariate techniques

The fragmentation of the original vegetation of the Cerrado biome, caused by the expansion of agricultural areas, mainly in central-west Brazil, calls for an assessment of the native population of this vegetation, especially of the species of interest for domestication and sustainable use. The purpose of this study was to characterize the genetic diversity of 140 gabiroba mother plants ( ... more

E.S. de Assis; E.Fdos Reis; J.F.N. Pinto; L.A.S. Contim; L.A.S. Dias
05/31/2011
Conservation; Genetic diversity; Microsatellites; Pelodiscus sinensis

Twenty-one novel polymorphic microsatellite loci were isolated from the Chinese soft-shelled turtle, Pelodiscus sinensis (Trionychidae). This is a commonly consumed Asian species, especially in China, where it is reared in large numbers on farms. We screened 34 unrelated individuals from Wuhu of Anhui province in China and detected high levels of polymorphism for all 21 loci, ... more

X.J. Bu; L. Liu; L. Wang; L.W. Nie
05/24/2011
AMOVA; Conservation; Endemism; Genetic diversity; Molecular markers

The bristle-spined porcupine, Chaetomys subspinosus, an endemic rodent from Atlantic Forest, was considered to be abundant in the recent past, but population reductions due to habitat loss and expansion of human activities caused this species to be included in the “vulnerable” category of the World Conservation Union Red List. We performed the first genetic assessment in natural ... more

C.G. Oliveira; R.A. Martinez; G.A.F. Giné; D.M. Faria; F.A. Gaiotto
09/10/2013
Conservation; Genetic diversity; Meliponini tribe; Microsatellite markers; Pollinators; Recôncavo Baiano

Bees play a key role in pollination and thereby help maintain plant diversity. The stingless bee Melipona scutellaris is an important pollinator in northeastern Brazil because it is endemic to this region. Both deforestation and timber harvesting have reduced the nesting sites for this species, thus reducing its population and range. Genetic studies may help reverse this process ... more

J.L. Viana; A.K. Francisco; C.A.L. Carvalho; A.M. Waldschmidt
06/13/2013
Bromeliaceae; Conservation; Endemism; Genetic diversity; Vriesea cacuminis

Data about the genetic structure can help to understand the evolutionary process of natural populations as well as to drive strategies of conservation. Vriesea cacuminis, an endemic Brazilian Bromeliad, has been found in 2 areas of Minas Gerais State. One is a legal preservation unit (Ibitipoca State Park) and the other an unprotected area (Serra Negra). The 2 areas belong to the ... more

P.C.C. Ribeiro; L.C. Pinheiro; R. Domingues; R.C. Forzza; M.A. Machado; L.F. Viccini
12/03/2012
Chuling; Conservation; Genetic diversity; Polyporus umbellatus; SRAP

Polyporus umbellatus (Pers.) Fries is an endangered medicinal fungus in China with in vivo anticancer activity, but its genetic information is lacking. Eight natural P. umbellatus strains collected from 7 provinces in China were subjected to sequence-related amplified polymorphism markers to estimate the level and pattern of genetic diversity. Forty- ... more

Y. Zhang; Y. Kang; Y. Qin; Z. Zhou; M. Lei; H. Guo
08/21/2013
Cagaita; Cerrado; Genetic diversity; Microsatellite; Shotgun library

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles ... more

M.P.C. Telles; J.B. Silva; L.V. Resende; R.P. Vianello; L.J. Chaves; T.N. Soares; R.G. Collevatti
12/08/2006
Bats; Chiroptera; Fluorescent in situ hybridization; Genetic variation; Random amplified polymorphic DNA

Random amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by ... more

P.R.L. Moreira; E. Morielle-Versute
04/05/2011
Chiroptera; Fluorescent in situ hybridization; NORs; Nucleologenesis; Nucleolus

We analyzed the behavior of the nucleolus, nucleolar structures and nucleolus organizer regions (NORs) during meiotic di­vision in four species of phyllostomid bats that have different num­bers and locations of NORs. Nucleoli began disassembly at leptotene, and the subcomponents released from the nucleolus were dispersed in the nucleoplasm, associated with perichromosomal regions, or they ... more

M.R. Beguelini; S.R.C. Marchesin; M.T.V. Azeredo-Oliveira; E. Morielle-Versute
03/15/2007
Acridine orange test; Bovine; Conservation; DNA; Freeze-drying sperm; TUNEL

The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. The AOT did not detect any differences between different ... more

C.F. Martins; M.N. Dode; S.N. Báo; R. Rumpf

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