Research Article

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Tick resistance in cattle is mainly found in zebu (Bos indicus) animals, although it is also present in some taurine (B. taurus) breeds. In order to characterize functional genes involved in tick resistance/susceptibility in cattle, two cDNA libraries were generated using skin tissues of selected Holstein x Gyr animals. A total of 2700 high-quality reads from both ... more

C.S. Nascimento; M.A. Machado; S.E.F. Guimarães; M.F. Martins; J.O. Peixoto; J. Furlong; M.C.A. Prata; R.S. Verneque; R.L. Teodoro; P.S. Lopes
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Bioinformatics; Cancer; Expressed sequence tags; Gene discovery; ORESTES

The Human Cancer Genome Project generated about 1 million expressed sequence tags by the ORESTES method, principally with the aim of obtaining data from cancer. Of this total, 341,680 showed no similarity with sequences in the public transcript databases, referred to as “no-match”. Some of them represent low abundance or difficult to detect human transcripts, but part of these ... more

Rda Silva Fonseca; D.Maria Carraro; H. Brentani
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Antioxidant; Expressed sequence tags; Free radicals; Oxidative stress; Paracoccidioides brasiliensis; Transcription factors

Survival of pathogenic fungi inside human hosts depends on evasion from the host immune system and adaptation to the host environment. Among different insults that Paracoccidioides brasiliensis has to handle are reactive oxygen and nitrogen species produced by the human host cells, and by its own metabolism. Knowing how the parasite deals with reactive species is important to ... more

É.G. Campos; Ada Silva Dantas; Mde Macedo Brigido; Mde Macedo Brigido; M.Sueli S. Felipe; R.Santos Amo Jesuino
06/30/2005
Dimorphic fungus; Expressed sequence tags; Metabolism; Paracoccidioides brasiliensis; Paracoccidioidomycosis; Transcriptome

Annotation of the transcriptome of the dimorphic fungus Paracoccidioides brasiliensis has set the grounds for a global understanding of its metabolism in both mycelium and yeast forms. This fungus is able to use the main carbohydrate sources, including starch, and it can store reduced carbons in the form of glycogen and trehalose; these provide energy reserves that are ... more

F.B.M. Arraes; B. Benoliel; R.T. Burtet; P.L.N. Costa; A.S. Galdino; L.H.A. Lima; C. Marinho-Silva; L. Oliveira-Pereira; P. Pfrimer; L. Procópio-Silva; V.CasteloBr Reis; M.Sueli S. Felipe
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Automatic annotation; Bioinformatics; Differential transcriptome; Expressed sequence tags; Paracoccidioides brasiliensis

Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an endemic mycosis of Latin America. This fungus presents a dimorphic character; it grows as a mycelium at room temperature, but it is isolated as yeast from infected individuals. It is believed that the transition from mycelium to yeast is important for the infective process. The Functional ... more

M.M. Brígido; M.Emilia M.T. Walter; M.Emilia M.T. Walter; A.G. Oliveira; M.K. Inoue; D.S. Anjos; E.F.O. Sandes; J.J. Gondim; M.José de A. Carvalho; N.F. Almeida; M.Sueli Soar Felipe
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cDNA; Expressed sequence tags; heavy metal; woody plant

Many cell functions are redundantly executed in cells, and the experimental approaches that analyze the group of proteins, whose expression is modified in a specific functional condition, enable the identification of the group of proteins that are expressed under stress conditions. The objective of the present study was the evaluation of the genetic expression induced by cadmium (Cd) in ... more

V.L. Souza; A.A.F. Almeida; B.T.Hora Júnior; B.T.Hora Júnior; A.S. Gesteira; J.C.M. Cascardo
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Diversity; Expressed sequence tags; Hevea brasiliensis; Microsatellite

Cultivated clones of Hevea brasiliensis have a narrow genetic base. In order to broaden the genetic base, it is first necessary to investigate the genetic diversity of wild populations. Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed to investigate the genetic diversity of Hevea populations. Four hundred and thirty microsatellites were ... more

Z.W. An; Y.C. Li; Q.L. Zhai; L.L. Xie; Y.H. Zhao; H.S. Huang
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Expressed sequence tags; Microsatellite; Paeonia delavayi; Population structure

Paeonia Franchet contains approximately 30 species of perennial herbs and is the only member of the family Paeoniaceae. Paeonia delavayi is an endangered ornamental plant that has a compound flower color and is endemic to southwest China. Its optimal habitats have been fragmented and its population size has greatly decreased as a result of human activities. Using a ... more

D.X. Wang; H. Ma; Y.L. Zhang; A.A. Duan; W.J. Li; Z.H. Li
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Expressed sequence tags; Genetic characterization; Manila clam; Microsatellites marker; Ruditapes philippinarum

Manila clam (Ruditapes philippinarum) is a valuable and intensively exploited shellfish species in Korea. Despite its importance, information on its genetic background is scarce. For the genetic characterization of R. philippinarum, expressed sequence tag-derived microsatellite markers were developed using next-generation sequencing. A total of 5879 tandem ... more

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Amino acid sequences; Expressed sequence tags; Gene expression; In silico analysis

Sweet orange (Citrus sinensis) plays an important role in the economy of more than 140 countries, but it is grown in areas with intermittent stressful soil and climatic conditions. The stress tolerance could be addressed by manipulating the ethylene response factor (ERF) transcription factors because they orchestrate plant responses to environmental stress. We performed an in ... more

T.M. Ito; P.B. Polido; M.C. Rampim; G. Kaschuk; S.G.H. Souza

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