Research Article

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12/13/2013
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Myogenic determination factor 1 (MyoD1) and myogenic factor 6 (Myf6) genes belong to the myogenic differentiation (MyoD) gene family, which play key roles in growth and muscle development. The study aimed to investigate the effects of variants in cattle MyoD1 and Myf6 on carcass and meat traits. We screened single nucleotide polymorphisms (SNPs ... more

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Osteoarthritis (OA) is the most prevalent form of arthritis in the elderly. This disease is characterized by breakdown and loss of articular cartilage due to genetic, mechanical and environmental factors. Although the pathophysiology of OA is not completely known, several candidate genes have been reported to be associated with OA susceptibility. We assessed the association between ... more

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Acenocoumarol; APOE; CYP2C19; CYP2C9; Genetic polymorphisms; PCR-RFLP

Oral anticoagulants of the coumarin type have an inconveniently narrow therapeutic window, making their use difficult. In Mexico, genetic variables that participate in the heterogeneity of the therapeutic response remain poorly investigated. With the focus on warfarin, extensive pharmacogenomic studies have been performed, including those on the CYP450 family and APOE. The ... more

J.A. Nastasi-Catanese; J.R. Padilla-Gutiérrez; Y. Valle; F. Ortega-Gutiérrez; M.P. Gallegos-Arreola; L.E. Figuera
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Growth traits; IGF-I; PCR-RFLP; Pectoralis major; Synthetic chicken; Thai native chicken

Four Thai synthetic chicken lines (Kaen Thong, Khai Mook Esarn, Soi Nin, and Soi Pet) originated from Thai native and exotic commercial chickens were evaluated for their growth and carcass traits with the purpose of developing a Thai broiler breeding program. Insulin-like growth factor I (IGF-I) gene is known to play an important role in growth, proliferation and differentiation ... more

N. Promwatee; B. Laopaiboon; T. Vongpralub; Y. Phasuk; S. Kunhareang; W. Boonkum; M. Duangjinda
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Kisspeptins, the product of the KISS1 gene, play an essential role in the regulation of reproductive functions, acting primarily at the hypothalamic level of the gonadotropic axis. We detected polymorphisms of the goat KISS1 gene in 723 individuals from three goat breeds (Xinong Saanen, Guanzhong, and Boer) by DNA pooling, PCR-RFLP, and DNA sequencing methods. We cloned ... more

X.P. An; P. Han; J.X. Hou; H.B. Zhao; Y. Yan; T. Ma; F. Fang; F.X. Meng; Y.X. Song; J.G. Wang; B.Y. Cao
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Clone; Duck; PCR-RFLP; PLIN gene; SNP

The perilipin (PLIN) gene is a candidate gene of carcass and fat traits in ducks. In order to study the molecular character of the PLIN gene and its function in different breeds of Chinese ducks, samples were obtained from the Chinese Academy of Agricultural Sciences Research Center for Birds, including 95 Peking ducks of the Z2 series, 91 Peking ducks of the Z4 series, ... more

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Centridini; PCR-RFLP; Population variation; Solitary bee

Centris spp are oil-collecting solitary bees that are valuable pollinators of crops such as Brazil nut, cashew, and acerola. We investigated the genetic variability of populations of C. aenea in the northeastern region of Brazil. Total DNA was extracted from 59 individuals from 6 locations in the States of Pernambuco and Bahia and a 600-650-bp fragment of the ... more

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Cattle; CDIPT gene; PCR-RFLP; SNP; Ultrasound measurement traits

The CDIPT is crucial to the fatty acid metabolic pathway, intracellular signal transduction and energy metabolism in eukaryotic cells. We detected three SNPs at 3'-untranslated regions (UTR), named 3'-UTR_108 A > G, 3'-UTR_448 G > A and 3'-UTR_477 C > G, of the CDIPT gene in 618 Qinchuan cattle using PCR-RFLP and DNA sequencing methods. At each of the three SNPs, we ... more

C.Z. Fu; H. Wang; C.G. Mei; J.L. Wang; B.J. Jiang; X.H. Ma; H.B. Wang; G. Cheng; L.S. Zan
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Chinese cattle; Dairy performance; IGF1 gene; PCR-RFLP

The insulin-like growth factor I (IGF-1) gene plays important roles in the growth and body composition of animals. Serum IGF1 concentration has been associated with growth traits in many livestock species. We found a polymorphism of cattle IGF1-TasI locus and analyzed the distribution of alleles in three cattle breeds, including Qinchuan, Nanyang, and Chinese Holstein. PCR-RFLP ... more

L.Z. Zhang; G. Ren; L.S. Hua; X.Y. Lan; C.Z. Lei; H. Chen
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Animal production; Growth traits; Molecular markers; PCR-RFLP

The aim of this study was to determine the association between a polymorphism in intron 3 of the bovine growth hormone (BGH) gene and growth traits related to the start of the reproductive life of Holstein heifers. This research was conducted using 480 Holstein heifers belonging to eight herds in three municipalities in the Department of Antioquia (Colombia). The phenotypic information ... more

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