Research Article

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12/12/2014
Alkaloids; Comet assay; DNA damage; Genotoxic activity; Genotoxicity test; Lupinus

The genus Lupinus is widely distributed. Its seeds are used for animal and human food, and Lupinus possesses pharmacological potential because of its high content of quinolizidine alkaloids and flavonoids; however, there is little available information about its genotoxicity. We used the comet assay and staminal nuclei of Tradescantia (clone 4430) to evaluate the ... more

M.R. Silva; C.M. Alvarez; P.M. García; M.A. Ruiz
09/08/2009
Comet assay; Medicinal plant; Micronucleus test; Single-cell gel electrophoresis; Tamarindus indica

Tamarindus indica has been used in folk medicine as an antidiabetic, a digestive aid, and a carminative, among other uses. Currently, there is no information in the toxicology literature concerning the safety of T. indica extract. We evaluated the clastogenic and/or genotoxic potential of fruit pulp extract of this plant in vivo in peripheral blood and liver ... more

F.M.V. Silva; M.F. Leite; A.C.C. Spadaro; S.A. Uyemura; E.L. Maistro
04/22/2009
Ambelania occidentalis; Comet assay; Micronucleus test; Mutagenicity assay; Single-cell gel electrophoresis

Ambelania occidentalis is routinely used in folk medicine for treating gastrointestinal disorders, even though there have been no safety trials. We evaluated the genotoxic potential of hydro-alcoholic extracts of this plant in mice; induced DNA damage was assessed in peripheral blood leukocytes and micronucleus induction was assessed in polychromatic erythrocytes from bone marrow. The ... more

L.S. Castro; F.F. Perazzo; E.L. Maistro
08/14/2008
Comet assay; Micronucleus test; Musa paradisiaca; peripheral blood cells; Single-cell gel electrophoresis

Plants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orally with three different concentrations of the extract (1000 ... more

C.U.B. Andrade; F.F. Perazzo; E.L. Maistro
07/29/2008
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Cattley guava (Psidium cattleyanum Sabine) is a native fruit of Brazil that is popular both as a sweet food and for its reputed therapeutic properties. We examined whether it could damage DNA using the alkaline single-cell gel electrophoresis (comet assay) and the micronucleus test in leukocytes and in bone marrow cells of mice. P. cattleyanum leaf extract was tested at ... more

T.D.A. Costa; S. Vieira; S.F. Andrade; E.L. Maistro
06/14/2011
Caffeic acid; Cinnamic acid; Clastogenic effects; Comet assay; Ferulic acid; Micronucleus test

Phenols are a large and diverse class of compounds, many of which occur naturally in a variety of food plants; they exhibit a wide range of biological effects, including antibacterial, anti-inflammatory, antiallergic, hepatoprotective, antithrombotic, antiviral, anticarcinogenic, and vasodilatory actions. We examined the genotoxic and clastogenic potential of three phenolic compounds: ... more

E.L. Maistro; J.P.F. Angeli; S.F. Andrade; M.S. Mantovani
10/26/2010
Chromosome aberrations; Comet assay; Essential oil; Micronucleus test; Rosmarinus officinalis

Rosmarinus officinalis (rosemary) oil is widely used by the cosmetic, food, and pharmaceutical industries as a fragrance component of soaps, creams, lotions, and perfumes. Although it is popular, potential harmful side-effects of the oil have been described. We investigated the genotoxic and mutagenic potential of essential oil of R. officinalis in rodents, using comet, ... more

E.L. Maistro; S.F. Mota; E.B. Lima; B.M. Bernardes; F.C. Goulart
05/21/2012
Ageing; Comet assay; GST genes; Micronucleus test; Multiplex PCR; Occupational exposure

We evaluated the possible influence of glutathione S-transferase mu (GSTM1) and glutathione S-transferase theta (GSTT1) genes on genetic damage due to occupational exposure, which contributes to accelerate ageing. This study was conducted on 120 car auto repair workshop workers exposed to occupational hazards and 120 controls without this kind of exposure. The null and non-null genotypes ... more

S.A. Eshkoor; S.J. Marashi; P. Ismail; S.A. Rahman; M. Mirinargesi; M.Y. Adon; R.V. Devan
01/26/2015
Bocaiúva; Comet assay; Micronucleus test; Oleic acid

Acrocomia aculeata (Jacq.) Lodd. ex Mart. is a plant species commonly used as a foodstuff and also for treating diseases, since it contains high concentrations of antioxidant compounds and monounsaturated fatty acids. Considering its ethnopharmacological relevance, the aim of the present study was to assess the cytotoxic, genotoxic, and mutagenic effects of an oil extracted from ... more

G.K. Traesel; L.H.A. Castro; P.V.B. Silva; R.M. Muzzi; C.A.L. Kassuya; A.C. Arena; S.A. Oesterreich
12/30/2003
Comet assay; Micronucleus test; Occupational exposure

The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exfoliated buccal cells were ... more

M.G. Martino-Roth; J. Viégas; D.M. Roth

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