Research Article

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ARMS-PCR; Genotyping; mutations; PCR-SSCP; PRNP alleles; Sheep

Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep breeds. We analyzed 432 sheep of five different breeds from ... more

C.L. Zhao; R. Wu; L. Liu; F.D. Li; X.L. Zhang; C. Wang; F. Wang; X.L. Diao; H.W. Guan; X. Wang; L. Zhou
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Candidate gene; Genotyping; PCR-RFLP; POU1F

We examined the polymorphisms in the PIT1 gene of 103 pigs and compared their frequencies in the maternal and paternal lineages of the Pietrain and Large White breeds, which have undergone divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by PCR and genotyped by RFLP, using the restriction enzyme RsaI. The data were ... more

A.C.P. Silveira; T.F. Braga; J.F. Almeida; R.C. Antunes; P.F.A. Freitas; A.S.M. César; A.S.M. César; E.C. Guimarães
10/03/2011
Association analysis; Candidate gene; Chicken; Egg number at 300 days of age; Polymorphism; QTL

We looked for variations that could be associated with chicken egg number at 300 days of age (EN300) in seven genes of the hypothalamic-pituitary-gonadal axis, including gonadotrophin-releasing hormone-I (GnRH-I), GnRH receptor (GnRHR), neuropeptide Y (NPY), dopamine D2 receptor (DRD2), vasoactive intestinal polypeptide (VIP), VIP receptor-1 ( ... more

H.P. Xu; H. Zeng; D.X. Zhang; X.L. Jia; C.L. Luo; M.X. Fang; Q.H. Nie; X.Q. Zhang
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Breeding value; Candidate gene; Chicken; Economic trait; Single nucleotide polymorphism

Neuropeptide Y (NPY) and gonadotrophin-releasing hormone receptor (GnRHR) are two candidate genes with a wide variety of physiological functions in growth and especially in reproduction processes. We examined the association of one SNP from each of these genes with growth- and egg production-related traits in Mazandaran native chickens. Two hundred and six individuals were genotyped by ... more

S.A. Fatemi; H. Mehrabani-Yeganeh; A. Nejati-Javaremi; S. Niknafs
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PRNP gene; Sheep; SNaPshot

Resistance and susceptibility to scrapie in sheep have been associated with SNPs located at codons 136, 154 and 171 of the prion protein (PRNP) gene. Many countries have sheep breeding programs selecting for resistance to scrapie based on the genotyping of these SNPs. We adapted a fast and robust method for genotyping sheep flocks for these polymorphisms, with reduced costs. Ninety-six ... more

P. Ianella; C.M. McManus; S.R. Paiva; A.R. Caetano
10/06/2006
Gene polymorphisms; MHC; OLA; Ovar-Mhc; Review; Sheep; Structure

The major histocompatibility complex (MHC) in sheep, Ovar-Mhc, is poorly characterised, when compared to other domestic animals. However, its basic structure is similar to that of other mammals, comprising class I, II and III regions. Currently, there is evidence for the existence of four class I loci. The class II region is better characterised, with evidence of one DRA, four ... more

V.S.R. Dukkipati; H.T. Blair; D.J. Garrick; A. Murray
09/29/2009
Cattle; Effective population size; Inbreeding; Pigs; Sheep; Software

Genetic variation provides a basis upon which populations can be genetically improved. Management of animal genetic resources in order to minimize loss of genetic diversity both within and across breeds has recently received attention at different levels, e.g., breed, national and international levels. A major need for sustainable improvement and conservation programs is accurate ... more

E. Groeneveld; Bv.d. Westhuizen; A. Maiwashe; F. Voordewind; J.B.S. Ferraz
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Caseous lymphadenitis; Corynebacterium pseudotuberculosis; Goat; Sheep; Vaccine
F.A. Dorella
04/03/2012
Gene expression; Hemi-nested PCR; Indirect immunofluorescence; Sheep

The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35) and embryos (N = 41) were obtained by laparotomy from adult ... more

J.V. Luz; A.S. Alcântara-Neto; R.I.T.P. Batista; J.M.G. Souza; D.I.A. Teixeira; L.M. Melo; V.J.F. Freitas
09/05/2014
DNA sequencing; GH gene; Growth traits; Polymerase chain reaction single-strand conformation polymorphism; Polymorphism; Sheep

The growth hormone gene plays an important role in the physiological function of an organism. The current study aimed to investigate the correlation between polymorphisms in the 5ꞌ regulatory region, exon 4, and 3ꞌ untranslated region (UTR) of the sheep GH gene and sheep growth traits. The DNA from 510 adult sheep was analyzed by DNA sequencing and polymerase chain reaction single ... more

J.L. Jia; L.P. Zhang; J.P. Wu; Z.J. Ha; W.W. Li

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