Research Article

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02/27/2014
Acroporidae; Gene flow; Genetic markers; Genotyping; Marker transferability; South-east Africa

Here, we report the successful cross-species amplification of previously published acroporid microsatellite markers in the coral Acropora austera from the south-western Indian Ocean. This fast-growing species is a major reef-building coral on South African reefs; however, it is the most damaged coral by scuba diving activity, and is known to be very susceptible to coral bleaching ... more

P.H. Montoya-Maya; A.H.H. Macdonald; M.H. Schleyer
05/11/2015
Baller-Gerold syndrome; Genotyping; Prenatal diagnosis; RECQL4

Birth defects are structural and/or functional malforma­tions present at birth that cause physical or mental disability and are im­portant public health problems. Our study was aimed at genetic analysis and prenatal diagnosis of congenital anomalies to understand the cause of certain birth defects. Karyotypes and array-comparative genomic hy­bridization (aCGH) were performed on a pregnant ... more

D.H. Cao; K. Mu; D.N. Liu; J.L. Sun; X.Z. Bai; N. Zhang; G.B. Qiu; X.W. Ma
08/08/2011
Germplasm; RAPD analysis; ULCV; Urdbean

Urdbean (Vigna mungo) is an important pulse crop grown worldwide. Urdbean leaf crinkle virus (ULCV) is a pathogen of urdbean found in Pakistan that causes huge losses in yield. Forty urdbean varieties/lines were screened against the virus under field conditions during spring season 2009. None of the lines appeared to be highly resistant or resistant. On the basis of a 0-5 disease ... more

R. Binyamin; A. Khan; A.I. Khan; A. Khan; F.S. Awan; N.A. Khan
08/28/2014
Breeding; Genotypic value; Germplasm; Manihot esculenta Crantz; Mixed models; Selection

The aim of this study was to estimate the genetic parameters and predict the genotypic values of root quality traits in cassava (Manihot esculenta Crantz) using restricted maximum likelihood (REML) and best linear unbiased prediction (BLUP). A total of 471 cassava accessions were evaluated over two years of cultivation. The evaluated traits included amylose content (AML), root ... more

E.J. Oliveira; F.A. Santana; L.A. Oliveira; V.S. Santos
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A priori choice of hybrid parents; Molecular markers; Morphological markers; Plant species

Plant breeding deals with high-yielding genotypes. However, how best to choose parents of these genotypes remains an unsolved question. Here, we focus on a priori choice based on parental distances by means of agronomic and molecular data. Despite numerous theoretical and empirical studies, a priori choice continues to be a controversial procedure. Both success and ... more

L.Antônio d Dias; E.Augusto de Picoli; R.Barros Rocha; A.Couto Alfenas
12/11/2007
Diversity; Ephedra; Ephedrine; Genetic distance; Molecular markers; Random amplified polymorphic DNA

Ephedra, also known as “ma huang”, is a dioecious, drought- and frost-resistant, perennial, evergreen shrub with compelling medicinal value. The genus is represented by 42 species around the world, 9 of which were provisionally reported from Pakistan. Species of the genus have a controversial taxonomy due to their overlapping morphological features. Conventional tools alone ... more

M.M. Shah; Z.A. Swati; S.H. Shah; A. Pervez; S. Ghafoor; H. Ahmad; U. Farooq
09/30/2007
Atlantic Forest; Brazil; Bristle/hair DNA extraction; Chaetomys subspinosus; Molecular markers; Threatened species

DNA extraction protocols are as varied as DNA sources. When it comes to endangered species, it is especially important to pay attention to all details that ensure the completion of the study goals and effectiveness in attaining useful data for conservation. Chaetomys subspinosus (Rodentia: Erethizontidae) is a secretive arboreal porcupine endemic to certain ecosystems of the ... more

C.G. Oliveira; R.A. Martinez; F.A. Gaiotto
03/09/2007
Azoospermia; Infertility; Molecular markers; Ring-Y

In the present study, we report on the case of a 43-year-old male patient seeking for fertility assistance, who showed a seminal analysis and testicular biopsy of complete azoospermia. Peripheral blood culture for chromosome studies revealed a karyotype of 46 chromosomes with a ring-Y-chromosome that lost the long arm heterochromatin. Molecular analysis of genomic DNA from the patient detected ... more

F.M. Carvalho; E.V. Wolfgramm; I. Degasperi; B.M. Verbeno; B.A. Vianna; F.F. Chagas; F. Paula; I.D. Louro; A.M.S. Perroni
08/11/2006
BoLA; Bovine; Candidate genes; Cattle; Molecular markers; Tick resistance

Losses caused by bovine tick burdens in tropical countries have a tremendous economic impact on production systems. Besides reducing production, this parasite can cause death in the most susceptible animals. The use of commercial acaricides has been the major method of control, but their misuse has led to tick resistance to many chemicals. More recently, vaccines have been used in ... more

M.L. Martinez; M.A. Machado; C.S. Nascimento; M.V.G.B. Silva; R.L. Teodoro; J. Furlong; M.C.A. Prata; A.L. Campos; M.F.M. Guimarães; A.L.S. Azevedo; M.F.A. Pires; R.S. Verneque
11/17/2009
Genetic polymorphisms; Lipid profile; Marker assisted selection; Molecular markers; Nellore cattle; Single nucleotide polymorphisms
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