Research Article

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Dactylis glomerata L.; Discriminatory power; DNA fingerprinting; Orchardgrass cultivar; Simple sequence repeats

The accurate identification of orchardgrass (Dactylis glomerata L.) cultivars is necessary to ensure purity for consumers, the effective utilization of cultivars, and to protect the intellectual property for breeders. Therefore, this study aimed to use SSR to construct DNA fingerprinting of orchardgrass cultivars. The genetic diversity of 32 orchardgrass cultivars originated ... more

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The genus Colletotrichum, comprised of pathogenic fungi that affect plants grown worldwide, causes the disease known as anthracnose in several fruit and vegetable species. Several studies conducted on plants have shown that the disease is characterized by the presence of one or several species of the fungus attacking the fruit or other organs of the same host. To develop and ... more

M.L. Marulanda; A.M. López; L. Isaza; P. López
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The bristle-spined porcupine, Chaetomys subspinosus, an endemic rodent from Atlantic Forest, was considered to be abundant in the recent past, but population reductions due to habitat loss and expansion of human activities caused this species to be included in the “vulnerable” category of the World Conservation Union Red List. We performed the first genetic assessment in natural ... more

C.G. Oliveira; R.A. Martinez; G.A.F. Giné; D.M. Faria; F.A. Gaiotto
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AMOVA; Dice; genetic relationships; Principal coordinate analysis; Random amplified polymorphic DNA; Wild and cultivated olives

We examined genetic relationships among wild and cultivated olives, which is a very important crop in the economy of the Aegean region. We used RAPD analysis to evaluate relationships among and within 22 olive subspecies from Manisa, Mugla and Izmir provinces in Turkey. Twelve of the subspecies were wild and 10 were cultivated olives. Fifty-two primers were used (OP-Q 1-20, OP-I 1-20, OP- ... more

M. Sesli; E.D. Yegenoglu
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We analyzed the genetic variability of 40 watermelon accessions collected from 8 regions of Northeastern Brazil using microsatellite markers, in order to suggest strategies of conservation and utilization of genetic variability in this species. These accessions are not commercial cultivars. They were sampled in areas of traditional farmers that usually keep their own seeds for future ... more

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Oenocarpus mapora is an Amazonian palm species commonly used by native populations for food and in folk medicine. We measured genetic variability, using RAPD markers, of material kept in a germplasm bank composed of accessions sampled from the Brazilian Amazon. These included 74 individuals from 23 accessions sampled from 9 localities in three States of the Brazilian Amazon. ... more

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AMOVA; Genetic diversity; ISSRs; RAPDs; Wild olive

Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 RAPDs and 10 ISSR markers. The mean value of ... more

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Dactylis glomerata L.; Optimization; Orchardgrass; Orthogonal design; Reaction system; Start codon target polymorphism-polymerase chain reaction

The effects of 5 factors (template DNA, Mg2+, dNTPs, Taq DNA polymerase, and primer) on the polymerase chain reaction (PCR) were investigated to optimize the start codon targeted polymor­phism (SCoT)-PCR system of Dactylis glomerata L., using an orthogo­nal design L16 (45). A suitable SCoT-PCR system for D. glomerata was ... more

B. Zeng; X. Huang; L.K. Huang; J. Zhang; H.D. Yan; D. Luo; H. Liang; Y. Yuan
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Chloroplast; Fragaria vesca; Genome; Molecular marker; Simple sequence repeats

The use of simple sequence repeats (SSRs), or microsatellites, as genetic markers has become popular due to their abundance and variation in length among individuals. In this study, we investigated linkage groups (LGs) in the woodland strawberry (Fragaria vesca) and demonstrated variation in the abundances, densities, and relative densities of mononucleotide, dinucleotide, and ... more

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Composite interval mapping; Fruit firmness; Shelf life; Simple sequence repeats; Single marker analysis

Breeding for better quality fruits is a major focus for tomatoes, which are continuously subjected to post-harvest losses. Several methods have been used to improve the fruit shelf life of tomatoes, including the use of ripening gene mutants of Solanum lycopersicum. We developed extended shelf-life tomato hybrids with better quality fruits using ripening mutants. Nine tomato ... more

K.N. Yogendra; P.H.Ramanjini Gowda

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