Technical Note

Related GMR Articles

Annamocarya sinensis; Endangered species; Genetic diversity; Microsatellite; Population structure

The endangered perennial plant Annamocarya sinensis (Dode) Leroy is a tertiary relict tree restricted to southeastern China and northern Vietnam. To explore endangerment mechanisms, develop protection strategies, and guide reintroduction efforts for this species, we investigated genetic diversity and population structure by surveying 70 individuals from three distinct ... more

Z.Y. Zhang; X.M. Pang; J.W. Han; Y. Wang; Y.Y. Li
Gene flow; Genetic diversity; Lepidoptera evolution; Management units; Microsatellite; Population genetics

We present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, Diatraea saccharalis (Fabricius). We isolated 16 microsatellite loci through an enriched genomic library protocol. After characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on average) and in the ... more

V.A.C. Pavinato; K.L. Silva-Brandão; M. Monteiro; M.I. Zucchi; J.B. Pinheiro; F.L.F. Dias; C. Omoto
Genetic diversity; Microsatellite; Molecular breeding; Popcorn; SSR locus; Zea mays

We analyzed genetic structure and diversity among eight populations of popcorn, using SSR loci as genetic markers. Our objectives were to select SSR loci that could be used to estimate genetic diversity within popcorn populations, and to analyze the genetic structure of promising populations with high levels of heterozygosity that could be used in breeding programs. Fifty-seven alleles (3 ... more

I.B.O. Eloi; C.A. Mangolin; C.A. Scapim; C.S. Gonçalves; M.F.P.S. Machado
Genetic diversity; Microsatellite; Miiuy croaker; Population structure

Genetic diversity and patterns of population structure of the miiuy croaker were investigated using SSR markers. A set of 10 microsatellite loci revealed 40 alleles; the number of alleles varied from 2 to 10 for each marker. A relatively high level of genetic variability was observed between miiuy croaker individuals. Genetic diversity was relatively high within populations with ... more

Y. Qin; D.Q. Sun; T.J. Xu; X.Z. Liu; Y.N. Sun
Freshwater fish; Genetic diversity; Mantel test; Microsatellite; Population genetic structure; Spatial autocorrelation

Geographical genetics allows the evaluation of evolutionary processes underlying genetic variation within and among local populations and forms the basis for establishing more effective strategies for biodiversity conservation at the population level. In this study, we used explicit spatial analyses to investigate molecular genetic variation (estimated using 7 microsatellite markers) of ... more

M.P.C. Telles; R.G. Collevatti; R.S. Braga; L.B.S. Guedes; T.G. Castro; M.C. Costa; N.J. Silva-Júnior; R.B. Barthem; J.A.F. Diniz-Filho
Amplified fragment length polymorphism; Genetic diversity; Jackfruit; Markers; Polymorphism

Artocarpus heterophyllus Lam., commonly called jackfruit, is a medium-sized evergreen tree that bears high yields of the largest known edible fruit. Yet, it has been little explored commercially due to wide variation in fruit quality. The genetic diversity and genetic relatedness of 50 jackfruit accessions were studied using amplified fragment length polymorphism markers. Of 16 primer ... more

S. Shyamalamma; S.B.C. Chandra; M. Hegde; P. Naryanswamy
Genetic marker; Larus saundersi; Microsatellite; Polymorphism; Saunders’s gull

Nine polymorphic microsatellite loci were isolated, using tetranucleotide repeat oligonucleotide probes from an enriched DNA library of the globally “vulnerable” Saunders’s gull (Larus saundersi), collected from the Yancheng coastal wetland, one of the three remaining breeding sites in China. Six breast muscle tissues and 16 blood samples from 22 gulls and eight eggshell membrane ... more

H.X. Jiang; S.L. Ren; Y.Q. Hou; M. Yang; X.B. Wu
Invasive species; Microsatellite; Polymorphism; Pseudorasbora parva

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The ... more

C. Zeng; Y. Gul; K. Yang; L. Cui; W.M. Wang; Z.X. Gao
Duchenne muscular dystrophy; Dystrophin gene; Han population genetics; Microsatellite; Polymorphism

To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and 2nd intron were found to have a high degree of polymorphism. ... more

R.F. Sun; Y.S. Zhu; J.L. Feng; Z. Tian; W.J. Kuang; Y. Liu; H.B. Zhang; S.B. Li
Genetic marker; Microsatellite; Pelophylax hubeiensis; Polymorphism

Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0. ... more

J.C. Yan; F.H. Zhu; H.L. Wu