Technical Note

Related GMR Articles

11/17/2009
Genetic diversity; Polymerase chain reaction; Random amplified polymorphic DNA; Sugarcane

Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clones, which are themselves related, contributing to the parentage ... more

F.A. Khan; A. Khan; F.M. Azhar; S. Rauf
10/13/2009
Blackthorn; Genetic diversity; Prunus spinosa; Random amplified polymorphic DNA

Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) ... more

Y. Erturk; S. Ercisli; D. Maghradze; E. Orhan; G. Agar
09/15/2009
Ficus; Genetic diversity; Random amplified polymorphic DNA; Wild fig

The fig tree (Ficus carica L.) is of significant socio-economic importance in Turkey, with 25% of the world’s fig production. Genetic variation and relationships among 14 wild-grown figs sampled from Coruh Valley in Turkey were characterized by random amplified polymorphic DNA (RAPD). Ninety-eight DNA fragments were scored after amplification of DNA samples with 13 random primers ... more

M. Akbulut; S. Ercisli; H. Karlidag
10/27/2009
Degree of divergence; Genetic diversity; Plantago; RAPD markers

Plantago ovata, popularly known as isabgol, has great commercial and medicinal importance due to thin rosy white membranous seed husk. Isabgol seeds and husks have emollient, demulcent and laxative properties. We used both biometric and molecular techniques to assess the genetic variability and relatedness of 80 germplasm accessions of Plantago spp (P. ovata, ... more

N. Singh; R.K. Lal; A.K. Shasany
10/27/2009
CABMV; genetic breeding; Genetic diversity; Germplasm characterization; Passion fruit; Resistance
C.B.M. Cerqueira-Silva
04/14/2009
Genetic diversity; Malus; Random amplified polymorphic DNA; Rootstocks; Turkey; UPOV

Two local (Vezir-1 and Vezir-2) and two standard (M9 and MM106) clonal apple rootstocks were compared using both morphological and molecular markers. International Union for the Protection of New Varieties of Plants criteria were used for morphological evaluation, which did not clearly separate these rootstocks. We tested 47 random decamer primers for random amplified polymorphic DNA analysis ... more

A. Koc; M. Akbulut; E. Orhan; Z. Celik; S. Bilgener; S. Ercisli
12/23/2008
DNA markers; Genetic diversity; Random amplified polymorphic DNA; Sunflower diversity

The genetic diversity among eight sunflower lines was determined through the estimation of the random amplified polymorphic DNA method. One hundred and fifty-six DNA fragments were generated by 20 random primers, for an average of about 7.8 bands per primer. Of these amplified DNA fragments, 104 were polymorphic among the eight sunflower lines. Nei and Li’s similarity matrix gave values from ... more

M.A. Iqbal; H.A. Sadaqat; I.A. Khan
08/05/2008
Aspergillus flavus; Genetic diversity; Inter-simple sequence repeats; Internal transcribed spacer; Molecular markers; Random amplified polymorphic DNA

The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification is nowadays performed by traditional techniques combined ... more

P.P. Batista; J.F. Santos; N.T. Oliveira; A.P.D. Pires; C.M.S. Motta; E.A.LunaAlves Lima
02/19/2008
Amphibia; Anthropogenic; Cytogenetic; Genetic diversity; Leptodactylidae; Random amplified polymorphic DNA

Cytogenetic and random amplified polymorphic DNA analyses carried out in the species Leptodactylus podicipinus, L. ocellatus, L. labyrinthicus, and L. fuscus from rural and urban habitats of the northwest region of São Paulo State, Brazil, showed that the karyotypes (2n = 22), constitutive heterochromatin distribution and nucleolus organizer region ... more

M.P. Arruda; E. Morielle-Versute
12/20/2011
Aegiphilla sellowiana; Genetic conservation; Genetic diversity; RAPD; Riparian population; Tropical tree

The Tibagi River, located in southern Brazil, is associated with a significant degree of environmental heterogeneity, along its 550 km extension. There is concern about the integrity of this river’s ecosystem, as human interference has been increasing. Aegiphila sellowiana (Lamiaceae) is an important pioneer tree species, commonly found near rivers; the fruit is consumed by ... more

C. Medri; E.A. Ruas; C.F. Ruas; P.S. Medri; M.E. Medri; P.M. Ruas

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