Research Article

Related GMR Articles

AMOVA; Citrullus lanatus; Conservation; Dendrogram; SSR

We analyzed the genetic variability of 40 watermelon accessions collected from 8 regions of Northeastern Brazil using microsatellite markers, in order to suggest strategies of conservation and utilization of genetic variability in this species. These accessions are not commercial cultivars. They were sampled in areas of traditional farmers that usually keep their own seeds for future ... more

R.N.Cde S. Gama; C.A.F. Santos; deC.S. Dias
Blood clam; Microsatellite; Population structure; SSR; Tegillarca granosa

The blood clam, Tegillarca granosa, is widely cultivated in China. We isolated 6 microsatellite loci from T. granosa and used them to investigate genetic diversity and population structure of 5 widely distributed populations of blood clam collected from eastern and southeastern China. The allele number per locus varied from 4 to 9, and the polymorphism information ... more

Y.J. Wang; Q.G. Zeng; L.N. Xu
Agronomic traits; Genetic variation; Gossypium hirsutum L.; ISSR; RAPD; SSR

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD primers ... more

Z. Noormohammadi; H.A. Farahani; M. Sheidai; S. Ghasemzadeh-Baraki; O. Alishah
cluster analysis; Cynodon dactylon; Genetic diversity; SSR

Fifty-five wild accessions of bermudagrass (Cynodon dactylon) were collected from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet), and their genetic diversity was analyzed using simple sequence repeat markers. A total of 267 polymorphic bands were detected with 18 primer combinations. The genetic similarity among the accessions ranged from 0.688 to 0.894 with an ... more

Y. Ling; X.Q. Zhang; X. Ma; S.Y. Chen; T.T. Chen; W. Liu
Cultivar identification; Molecular markers; Pennisetum glaucum; Seed piracy; SSR

The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy. Thus, with the objective of identifying different lots with regard to origin, we characterized six ... more

R.P.Mendonça Neto; E.V.R. Von Pinho; B.L. Carvalho; G.S. Pereira
DNA extraction; Leguminosae; RAPD; SRAP; SSR

Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding liquid nitrogen usage and ... more

I.O. Agbagwa; S. Datta; P.G. Patil; P. Singh; N. Nadarajan
Anopheles albitarsis; Cross-amplification; Malaria vectors; Microsatellite-enriched library; Species complex; SSR

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897 ... more

G.M. Guimarães-Marques; J.S. Batista; H.M. Guimarães; M.V. Naice-Daou; M.P. Lima; K.M. Formiga; J.M.M. Santos; C.A.P. Lima; M.S. Rafael
cluster analysis; Genetic diversity; Lagerstroemia; SSR

Lagerstroemia (crape myrtle) are famous ornamental plants with large pyramidal racemes, long flower duration, and diverse colors. However, little is known about the genetic structure and diversity of germplasm in Lagerstroemia. We genotyped 81 L. indica cultivars, five other species of Lagerstroemia, and 10 interspecific hybrids using 30 simple sequence ... more

D. He; Y. Liu; M. Cai; H.T. Pan; Q.X. Zhang; X.Y. Wang; X.J. Wang
corn; Exserohilum turcicum; Gene mapping; Northern corn leaf blight; SSR

The gene HtNB confers non-lesion resistance to the fungal pathogen Exserohilum turcicum in maize. To map this gene, we developed two F2 populations, P111 (resistant line) x HuangZao 4 (susceptible line) and P111 x B73 (susceptible). HtNB was located on chromosome 8.07 bin, flanked by MAC216826-4 and umc2218 at distances of 3.3 and 3.4 cM, respectively ... more

H. Wang; Z.X. Xiao; F.G. Wang; Y.N. Xiao; J.R. Zhao; Y.L. Zheng; F.Z. Qiu
Cross-amplification; Fabaceae; Genetic diversity; Microsatellite libraries; SSR; Tree

Parapiptadenia rigida, locally known as angico, is a tropical tree common in the semideciduous Brazilian forest. Its wood is naturally resistant to insect attack and is useful for construction. Extracts from the tree have medicinal properties. We characterized nine microsatellite loci for P. rigida. Thirty-five alleles were detected in a sample of 45 individuals from 3 ... more

B.F. Costa; L.A. Rodrigues; E.A. Ruas; L.B. Souza; C.F. Ruas; B.G. Vieira; A.R.O. Conson; P.M. Ruas