Research Article

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Genetic diversity; Genetic structure; SSR

Nine microsatellite loci were isolated from the genome of Laodelphax striatellus (Homoptera: Delphacidae) by constructing (TC)6(AC)5 and (AG)6(AC)5 compound SSR-enriched libraries using suppression-PCR procedures. These loci were found to be highly polymorphic, with 13 to 30 alleles per locus in the three populations that we investigated ... more

J.T. Sun; J.B. Li; X.M. Yang; X.Y. Hong
05/21/2012
Chicken; EST; Gallus gallus; Genomics; Microsatellites; SSR

Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity in this species. The explosion of genomics and functional ... more

M.R. Bakhtiarizadeh; B. Arefnejad; E. Ebrahimie; M. Ebrahimi
04/10/2012
DNA fingerprinting; Fruit-endocarp or fruit-pit characters; Genetic diversity; Olea europaea; SSR

Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of 6.25 alleles per primer pair (a total of 25 ... more

F.J. Delgado-Martinez; I. Amaya; J.F. Sánchez-Sevilla; M.C. Gomez-Jimenez
04/27/2012
False-positive exclusion; PCR; Single-primer PCR; SSR

Polymerase chain reaction (PCR) is the foundation of SSR molecular marker technology. We used sib rice varieties J518, XD1 and SD23 as experimental materials, selecting 30 pairs of SSR primers, including RM127, RM337 and RM5172, covering the rice genome, and performed single- and double-SSR primer combined analyses. We found that under the same PCR system and conditions, a single primer ... more

J. Ma; S.C. Guan; Z. Zhang; P.W. Wang
04/14/2014
Molecular markers; SSR; Transferability; Tuber crop; Yams

Dioscorea cayenensis and Dioscorea rotundata are among the most important yam species for the humid and sub-humid tropics. We isolated nine polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. The nine primer pairs were validated in 22 D. cayenensis accessions, and were tested for transferability in 26 D. rotundata ... more

L.R.G. Silva; M.M. Bajay; M. Monteiro; T.F. Mezette; W.F. Nascimento; M.I. Zucchi; J.B. Pinheiro; E.A. Veasey
01/28/2014
Dactylis glomerata; Hybrids; Morphological characters; SSR

Six F1 populations derived from crosses among 4 orchardgrass (Dactylis glomerata L.) cultivars were studied by morphological and simple sequence repeat molecular markers to test for hybrid vigor and a correlation between genetic distance and heterosis. Heterosis was observed for days to length of culm, leaf traits, tiller numbers, etc. Significant differences between ... more

Y.F. Zhao; X.Q. Zhang; X. Ma; W.G. Xie; L.K. Huang
03/12/2014
Cross-species transferability; Pinus massoniana; SSR

Pinus massoniana (Masson’s pine) is a widespread tree species in central and southern China and northern Vietnam; it is valued for rosin and paper production. Despite the significant economic value of Masson's pine, little work has been done on its molecular genetics. We developed 318 SSR primers from genome sequences of P. massoniana, and we identified 10 polymorphic ... more

Y.H. Feng; Z.Q. Yang; J. Wang; Q.F. Luo; H.G. Li
01/08/2014
EST-SSR; Genetic diversity; SSR; Tomato varieties

The genetic diversity and relationship of 42 tomato varieties sourced from different geographic regions was examined with EST-SSR markers. The genetic diversity was between 0.18 and 0.77, with a mean of 0.49; the polymorphic information content ranged from 0.17 to 0.74, with a mean of 0.45. This indicates a fairly high degree of diversity among these tomato varieties. Based on the ... more

N.K. Korir; W. Diao; R. Tao; X. Li; E. Kayesh; A. Li; W. Zhen; S. Wang
06/01/2015
Cultivar identification; Genetic diversity; Genetic similarity; ISSR; Olea europaea L.; SSR

The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples ... more

M.M. Zhan; Z.Z. Cheng; G.C. Su; A.Y. Wang; H.P. Chen; Z.S. Yang; Z. Shan; Q.M. Huang
01/16/2015
Assisted selection; Citrullus lanatus; Fruit external pattern; SSR

Agronomic performance and external and internal appearance of watermelon (Citrullus lanatus) fruit are important traits that should be taken into consideration during the development of a new cultivar, as well as being the principal identification elements used by the consumer, which are based on the external appearance and quality of the fruit. Externally, the fruit can be ... more

R.N.C.S. Gama; C.A.F. Santos; R.C.S. Dias; J.C.S.F. Alves; T.O. Nogueira

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