Research Article

Related GMR Articles

Chicken; Gene expression profile; Liver

In this study, we profiled gene expression in chicken liver and screened differentially expressed genes in the Bai’er layers and fat line broilers. Birds were derived from the 14th generation of Northeast Agricultural University fat broiler lines and Bai’er layers. Chicken genome arrays were used to screen differentially expressed genes in liver tissue from the Bai’er layers and fat line ... more

Q.G. Wang; H.F. Zhang; S.Z. Wang; G.L. Gao; L. Leng; W. Na; H. Li
Association analysis; Candidate gene; Chicken; Egg number at 300 days of age; Polymorphism; QTL

We looked for variations that could be associated with chicken egg number at 300 days of age (EN300) in seven genes of the hypothalamic-pituitary-gonadal axis, including gonadotrophin-releasing hormone-I (GnRH-I), GnRH receptor (GnRHR), neuropeptide Y (NPY), dopamine D2 receptor (DRD2), vasoactive intestinal polypeptide (VIP), VIP receptor-1 ( ... more

H.P. Xu; H. Zeng; D.X. Zhang; X.L. Jia; C.L. Luo; M.X. Fang; Q.H. Nie; X.Q. Zhang
Chicken; Cross-species amplification; Grey partridge; Microsatellite markers; Turkey

We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even though we used cross-species amplification, a high degree of ... more

N. Bech; C. Novoa; J.F. Allienne; J. Boissier
Avian influenza virus; Chicken; Mx gene; PCR-RFLP; S631N mutation

The Mx (myxovirus resistance) gene codes for a protein with antiviral activity. Non-synonymous G/A polymorphism at position 2032 of chicken Mx cDNA results in a change at amino acid 631 of the Mx protein. This mutation has been shown to affect the antiviral activity of the Mx molecule, although recent studies have not confirmed this effect in response to some influenza ... more

L. Sironi; P. Ramelli; J.L. Williams; P. Mariani
Chicken; L-BABP; L-FABP; Lipid deposition

Liver fatty acid-binding protein (L-FABP) and liver bile acid-binding protein (L-BABP), in the liver intra-cytoplasm of chicken, are members of the fatty acid-binding protein subfamily. This study was designed to analyze and compare L-FABP and L-BABP expression levels between fat and lean lines in chicken liver tissue, and to determine the relationship between their expression and lipid ... more

Q. Zhang; H. Shi; W. Liu; Y. Wang; Q. Wang; H. Li
Blood genome; Chicken; Methylation pattern; MSAP

We used methylation-sensitive amplified polymorphism to examine DNA methylation levels and CCGG patterns in parents and offsprings of 3 groups of adult chickens, purebred White Leghorn (AA), White Plymouth Rock (EE), and crossbred individuals (EA) using 10 primer combinations. We found that about 66% of the cytosines at CCGG sites were not methylated. Fully methylated sites were less ... more

Q. Xu; D.X. Sun; J.L. Li; R. Liu; Y.C. Wang; Y. Zhang
Calcium; Chicken; meat quality; Mutation; Porcine stress syndrome; Ryanodine receptor

The development of pale, soft, and exudative (PSE) breast fillet meat has become an economic burden for the poultry industry worldwide. PSE meat results in 1.0-1.5% loss in moisture and carcass weight, and a 2010 estimate of the Brazilian annual production put the economic loss due to PSE at over US$30 million. In the USA, PSE has caused an annual loss of up to US$200 million to the ... more

F.G. Paião; L.M. Ferracin; M. Pedrão; T. Kato; M. Shimokomaki
Chicken; Cloning; Expression; Nramp1 gene; Nramp2 gene; Polymorphism

Natural resistance-associated macrophage protein 1 and 2 encoding genes (Nramp1 and Nramp2) are related to many diseases. We cloned the cDNA of chicken Nramp1 and Nramp2 genes, characterized their expression and polymorphisms, and investigated the association of some SNPs with resistance to salmonellosis. The Nramp1 cDNA was 1746 bp long and the ... more

X.M. He; M.X. Fang; Z.T. Zhang; Y.S. Hu; X.Z. Jia; D.L. He; S.D. Liang; Q.H. Nie; X.Q. Zhang
Chicken; Economic traits; Growth and meat quality association; HMGCR gene; Single nucleotide polymorphisms

The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is rate-limiting for metabolism of cholesterol; it plays an important role in endogenous cholesterol biosynthesis. We used DNA sequencing technology and created restriction site PCR-RFLP to detect HMGCR SNPs in an F2 resource population of Gushi chicken and Anka broilers. We found a G/T mutation (Gln/ ... more

Y. Wei; S.K. Zhu; S. Zhang; R.L. Han; Y.D. Tian; G.R. Sun; X.T. Kang
Chicken; Expression profiles; Hypothalamus; miRNA; Solexa sequencing

We used Solexa sequencing technology to identify and determine the abundance of miRNAs and compared the characteristics and expression patterns of miRNA of 1-day-old and 36-week-old chicken hypothalamuses. We obtained 17,825,753 and 10,928,745 high-quality reads from 36-week-old and 1-day-old chickens, respectively. Three hundred and seventy-one conserved miRNAs were expressed in both ... more

G.R. Sun; M. Li; G.X. Li; Y.D. Tian; R.L. Han; X.T. Kang