Research Article

Related GMR Articles

Acute promyelocytic leukemia; Cryptic insertions; Fluorescence in situ hybridization; promyelocytic leukemia/retinoic acid receptor alpha; Reverse transcription-polymerase chain reaction; Sequencing analysis

Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17)(q22;q11-21), resulting in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARα) genes. Using conventional cytogenetic methods, these translocations are normally detected in about 70-90% of patients; most negative results are due to technical problems or ... more

A. Choughule; S. Polampalli; P. Amre; S. Shinde; S. Banavali; K. Prabhash; R. Nair; P.G. Subramanian; S. Gujral; P.M. Parikh
Aegilops biuncialis; C-banding; chromosome; Fluorescence in situ hybridization; Karyotype

Aegilops biuncialis can be hybridized with wheat (Triticum spp) and has been used for wheat breeding and genetic studies. The A. biuncialis karyotype (Ub Ub Mb Mb) was investigated based on three A. biuncialis accessions grown in China. Two pairs of SAT chromosomes were identified as 1Ub and 5Ub ... more

J. Wang; W. Zhang; H. Zhao; F.R. Li; Z.G. Wang; J. Ji; X.Q. Zhang; D.W. Wang; J.M. Li
Cross-amplification; Fluorescence in situ hybridization; Forage; Microsatellite

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are ... more

C.A. Polido; C.C. Mantello; A.P. Moraes; A.P. Souza; E.R. Forni-Martins
Fluorescence in situ hybridization; Freshwater fishes; Karyotype evolution; Molecular cytogenetics; Ribosomal DNA

In the present study, the karyotype and chromosomal characteristics of 9 species of the Bagridae fish family were investigated using conventional Giemsa staining as well as dual-color fluorescence in situ hybridization to detect the 18S and 5S rDNA sites. In addition to describing the karyotype of several Bagridae catfishes, we established molecular cytogenetic techniques to ... more

W. Supiwong; T. Liehr; M.B. Cioffi; A. Chaveerach; N. Kosyakova; X. Fan; T. Tanee; A. Tanomtong
Fluorescence in situ hybridization; Infertility; Reciprocal translocation; Sperm aneuploidy rates

A reciprocal translocation between the short arm of chromosome 1 and the long arm of chromosome 3 was observed in a pedigree of three carriers (proband, and his brother and mother). In this study, the three carriers had different clinical manifestations: the proband with infertility, his brother with spousal miscarriages, and his mother with no adverse reproductive history. Cytogenetic ... more

L.L. Li; Y. Dong; R.X. Wang; N. An; X. Yun; R.Z. Liu
13q deletion; Chromosomal microarray analysis; Complex phenotype; Fluorescence in situ hybridization; Karyotype; Ring chromosome 13

We describe the first postnatal diagnosis of a child from Central Brazil with de novo cytogenetic alterations in 13q showing malformations of the brain, eyes, distal limbs, and genitourinary tract, and severe intellectual disability. The karyotype was a constitutive 46,XX,r(13)[77]/45,XX,-13[17]/46,XX,idic r(13)[6]. Interphase and metaphase fluorescence in situ ... more

L.B. Minasi; I.P. Pinto; J.G. de Almeida; A.V. de Melo; D.M.C. Cunha; C.L. Ribeiro; G.P. Silva; M.G. Brasil; D.M. Silva; C.C. da Silva; A.D. da Cruz
Cancer patients; Comparative genomic hybridization; Fluorescence in situ hybridization; Molecular cytogenetics

As the pioneer among molecular cytogenetics techniques, fluorescence in situ hybridization (FISH) allows identification of specific sequences in a structurally preserved cell, in metaphase or interphase. This technique, based on the complementary double-stranded nature of DNA, hybridizes labeled specific DNA (probe). The probe, bound to the target, will be developed into a ... more

M. Mühlmann; M. Mühlmann
Comparative genomic hybridization; Embryos; Fluorescence in situ hybridization

We studied chromosomal abnormalities in arrested embryos produced by assisted reproductive technology with fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in order to determine the best technique for evaluating chromosomal aneusomies to be implemented in different situations. We examined individual blastomeres from arrested embryos by FISH ... more

M.C. Muhlmann; A.O. Laudicina; C. Perandones; M.V. Bertolino; A. Marazzi; C.J. Quintans; M. Donaldson; W. Bozzo; S. Pasqualini
Fluorescence in situ hybridization; Molossid; Phyllostomid; Chiroptera; Telomeric sequence

Molossidae species, Cynomops abrasus (2n = 34, fun­damental number, FN = 64), Eumops auripendulus (2n = 42, FN = 62), Molossus rufus (2n = 48, FN = 64), Molossops temminckii (2n = 48, FN = 64), and Nyctinomops laticaudatus (2n = 48, FN = 64), and Phyl­lostomidae species, Phyllostomus discolor (2n = 32, FN = 60), have karyotypes with ... more

K.C. Faria; S.R.C. Marchesin; P.R.L. Moreira; M.R. Beguelini; E. Morielle-Versute
deletion; Essential hypertension; Fluorescence in situ hybridization; subtelomere; telomere

Telomere biology is intimately linked to the genetic/environmental etiology of cardiovascular and metabolic diseases and telomere shortening is emerging as an important biomarker disease. The relationship between subtelomeric deletions and genetic hypertension was examined. Fluorescence in situ hybridization was used to directly assess whether there is a loss or gain of subtelomere ... more

S.T. Onrat; A.G. Tomatir