Research Article

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04/02/2013
Apoptosis; Invasion; miR-181b; PC-3; Proliferation; Prostate cancer

We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC ... more

L. He; H. Yao; L.H. Fan; L. Liu; S. Qiu; X. Li; J.P. Gao; C.Q. Hao
11/28/2012
Apoptosis; Gene and protein expression; MGC; shRNA; siRNA; VEGF

Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonucleotides targeting the ... more

N. Ally; X.L. Zou; B.C. Jiang; L. Qin; L. Zhai; P. Xiao; H.L. Liu
08/24/2012
14-3-3 Proteins; Antioxidant; Apoptosis; Glioma; Heat shock protein 27 (Hsp27); Viscum album

Extracts of mistletoe (Viscum album) are intensively used in complementary medicine, but their mechanisms are not fully understood in most cases, and the effects on metabolism have not been investigated in detail. However, some biologically active natural products are well known to provoke unexpected cellular responses. They reduce overexpression of heat shock proteins (Hsps) in ... more

E.Ö. Uçar; N. Arda; A. Aitken
07/19/2012
Apoptosis; Fusion protein; hA20; High glucose; HUVECs; Protein transduction domain

We constructed a plasmid containing a protein transduction domain (PTD) and a human A20 (hA20) gene fragment; the fusion protein was obtained by highly expressing this plasmid in the yeast Pichia pastoris GS115. The plasmid was obtained by adding 9xArg and EcoRІ recognition sites to the end of the primer, and 6xHis-Tag and NotІ recognition sites to its end. ... more

C.L. Hou; Q. Huang; Y. Wei; W. Zhang; J.H. Mi; D.J. Ying; Z.H. Zhou
12/18/2014
Apoptosis; Atorvastatin; Cerebral ischemia and reperfusion; Cytochrome C; Mitochondrial permeability transition pore

The aim of this study was to investigate the influence of atorvastatin on the opening of the mitochondrial permeability transition pore (MPTP) and the expression of cytochrome C (Cyt C) in Sprague-Dawley rats with cerebral ischemia-reperfusion (I/R). The rat model of cerebral artery ischemia was established by the suture-occluded method with ischemia for 2 h and reperfusion for 72 h. ... more

T. Song; J. Liu; X. Tao; J.G. Deng
12/19/2014
Apoptosis; Cytochalasin B; Glioma; mitosis

Cytochalasin B (CB) is known to inhibit a number of cancer types, but its effects on gliomas are unknown. We examined the in vitro effects of CB on the proliferation of human glioma U251 cells, as well as determined its mechanism of action. Cell proliferation was determined using CCK-8. The effect of CB on U251 cell morphology was observed under a transmission electron microscope ... more

Z.G. Tong; N. Liu; H.S. Song; J.Q. Li; J. Jiang; J.Y. Zhu; J.P. Qi
11/28/2014
Apoptosis; BMPRIB; Porcine granulosa cells; siRNA; Steroidogenesis

Bone morphogenetic proteins (BMPs) are the key factors in maintaining cell growth and differentiation in ovaries. BMPs initiate signaling by assembling BMP receptors and activating Smads, which in turn alter the expression of target genes. However, little is known about the effect of the deletion of the Bone morphogenetic protein receptor type IB (BMPRIB) on porcine granulosa cell (GCs ... more

Y.Y. Zhao; X.X. Li; W. Wang; X. Chen; P. Yu; J.J. Wang; Y.X. Xu
10/31/2014
Antigenotoxicity; Apoptosis; Necrosis; Tephrosia cinerea

Tephrosia cinerea L. (Pers.) is a tropical species that exhibits antileishmanial activity in Leishmania amazonensis promastigote cultures and is commonly used to treat infections, inflammations, ulcers, nervous conditions, and diarrhea. However, no studies have investigated its effects on genetic material. Therefore, we evaluated the genotoxic potential, antigenotoxic ... more

A.C.S. Dias; V.R. Moreira; L.P. Almeida; M.I.S. Lima; J.L. Bezerra; M.N.S. Ribeiro; F.R.F. Nascimento; S.R.F. Pereira
08/28/2014
Apoptosis; Gastric cancer; Small interfering RNA; Survivin

A pair of inverted repeated sequences of the gene survivin was designed for stable double-stranded RNA establishment. After stable transfection, the biological behaviors of gastric cancer cells were observed. The interference rates of survivin-targeting siRNA (siRNA-survivin) in BGC823, MKN45, SGC7901, and cisplatin-resistant SGC7901 groups were 55.363 ± 3.974 ... more

Y.H. Li; M. Chen; M. Zhang; X.Q. Zhang; S. Zhang; C.G. Yu; Z.M. Xu; X.P. Zou
07/29/2014
Acrylonitrile; Apoptosis; Brain neuron; Neuron morphology; Rat

This study aimed to evaluate the effects of acrylonitrile (ACN) on neuronal morphology and apoptosis in rats. An ACN solution was administered to Wistar rats by gavage at doses of 0, 5, 10, or 20 mg/kg, 5 days a week for 13 weeks. The morphology of neurons and the presence of apoptosis was examined by light and electron microscope, DNA electrophoresis, immunohistochemistry, and terminal ... more

C.Z. Li; L.A. Tse; Y.L. Zhou; W. Fan; M. Li; I.T.S. Yu; T.Y. Jin

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