Research Article

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12/16/2008
Conservation; Endangered species; Mitochondrial DNA; Oryx; Phylogenetic relationship

The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the topologies of respective ... more

H.A. Khan; I.A. Arif; A.A.Al Homaidan; A.H.Al Farhan
09/27/2011
bottleneck; Endangered species; Microsatellite marker; Tanichthys albonubes

We developed 12 microsatellite loci for the endangered minnow species, Tanichthys albonubes, using PCR-based isolation of microsatellite arrays. These new markers were tested in 26 individuals from a wild population collected from Guangzhou in China and 26 individuals from a cultured strain. The number of alleles ranged from two to nine and the expected heterozygosity from 0.177 ... more

J. Zhao; D.Y. Tseng; H.D. Lin; X.T. Lin
10/10/2013
Dominant marker; Endangered species; Fragmentation; Genetic structure; Tropical forest

We analyzed the genetic diversity of populations of two sympatric species of Lymania (Bromeliaceae), both endemic to the Atlantic rainforest of southern Bahia (Brazil). Lymania azurea has a restricted occurrence, while Lymania smithii has a wider distribution. Our aim was to provide genetic data to contribute to the design of more efficient conservation ... more

V.C.C. Pamponét; T.F. Alves; R.A. Martinez; R.X. Corrêa; F.A. Gaiotto
09/27/2013
Annamocarya sinensis; Endangered species; Genetic diversity; Microsatellite; Population structure

The endangered perennial plant Annamocarya sinensis (Dode) Leroy is a tertiary relict tree restricted to southeastern China and northern Vietnam. To explore endangerment mechanisms, develop protection strategies, and guide reintroduction efforts for this species, we investigated genetic diversity and population structure by surveying 70 individuals from three distinct ... more

Z.Y. Zhang; X.M. Pang; J.W. Han; Y. Wang; Y.Y. Li
08/21/2013
Endangered species; Genetic diversity; Mergus squamatus; Wintering habitat

The scaly-sided merganser (Mergus squamatus), found in temperate East Asia, has been reduced to a very small population. Central and southern China are its main wintering habitat. However, populations have declined greatly since the 1980s due to habitat loss and degradation, and poaching. To meet the urgent need for up-to-date conservation information, we examined RAPD DNA ... more

Z.Q. Zhang; C.J. Qin; D.A. Fang; A.P. Wang; C.H. Luo; K. Liu
08/16/2012
Caldesia grandis; conservation genetics; Endangered species; Spatial genetic structure

The endangered marsh herb, Caldesia grandis, is native to China. We investigated the spatial structure of the genetic variation of three populations of C. grandis using RAPD markers and spatial autocorrelation analysis, based on the method of equal distance interval. A total of 157 individuals were sampled from four patches collected from the region of Hunan ... more

X.L. Yue; J.M. Chen; Y.H. Guo; Q.F. Wang
05/22/2012
Atlantic rainforest; DNA extraction protocol optimization; Endangered species; Molecular marker

Melanoxylon brauna (Fabaceae - Caesalpinioideae) is an endemic and valuable hardwood tree species in the Brazilian Atlantic rainforest; it is comparable to African ebony wood. We tested three protocols of DNA extraction based on the citrimonium bromide (CTAB) method and evaluated the quantity, purity and integrity of the DNA. We also determined whether these procedures interfere ... more

D.B. Borges; M.B. Amorim; A.M. Waldschmidt; E. Mariano-Neto; C.V. Vivas; D.G. Pereira
12/04/2014
Cactaceae; Endangered species; Perfect microsatellite markers; Rare plants

The cactus genus Uebelmannia includes 3 narrow endemic species associated with rocky savanna habitats in eastern South America. Because of their rarity and illegal over-collection, all of these species are endangered. Taxonomic uncertainties resulting from dramatic local variation in morphology within Uebelmannia species preclude effective conservation efforts, such as ... more

E.M. Moraes; F.W. Cidade; G.A.R. Silva; M.C. Machado
11/24/2014
Cytochrome b; Endangered species; Fishery product identification; Polymerase chain reaction-restriction fragment length polymorphism

Endangered angelsharks and a protected dolphin species are illegally traded in Brazil. In this study, we determined prospective molecular markers for detecting these species in the trade of angelshark carcasses and ‘dolphin’ eyeball amulets. We compiled publicly available as well as new and unpublished cytochrome b (cyt b) DNA sequences for species involved in these ... more

L.H.O. Falcão; M.A.A. Furtado-Neto; R. Maggioni; V.V. Faria
06/01/2015
Allozymes; Conservation; Elaeagnus mollis; Endangered species; Population genetic structure

Elaeagnus mollis Diels is a group of shrubs and dwarf trees endemic to China and are endangered plants. However, the reason why these plants are endangered remains controversial. The current study aimed to explore the endangered status of E. mollis from a genetic perspective and to propose conservation strategies for this species. Using 16 polymorphic allozyme loci, the ... more

J.M. Zhang; F. Zhang

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