Research Article

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A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H, D, and S). Similarity analysis shows that Es-CLSP ... more

Q.Z. Yang; Z.J. Yang; Y. Zhang; X.L. Li; W. Zhang
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Adhesion; Aeromonas hydrophila; ELISA; flgN; Mini-Tn10Km

Adhesion to the host mucus is a crucial step in the early infection stage of pathogenic bacteria. To investigate the mechanisms of the adhesion of Aeromonas hydrophila to its host mucus, a mutant library was constructed using the mini-Tn10 transposon mutagenesis system. Of 276 individual colonies, the mutant strain with the most attenuated adhesion ability in this study ... more

X. Jiang; Y.X. Qin; G.F. Lin; L. Huang; B. Huang; W.S. Huang; Q.P. Yan
02/06/2015
Aeromonas hydrophila; Aquareovirus; Grass carp; IL-12 p35; IL-12 p40; mRNA expression

The grass carp (Ctenopharyngodon idella) aquaculture industry in Asia is prone to bacterial and viral hemorrhagic diseases. Effective adjuvants for vaccine formulation are the need of the hour for control of these diseases and long-term sustainability of grass carp farming. In this study, the involvement of interleukin-12 (IL-12) from grass carp (gcIL‑12) in anti-bacterial and ... more

N.P. Pandit; Y.B. Shen; X.Y. Xu; H.Y. Yu; W.J. Wang; R.Q. Wang; Y.F. Xuan; J.L. Li
04/19/2012
Eriocheir sinensis; Na+-K+-ATPase; qRT-PCR; Salinity stress

Eriocheir sinensis is a euryhaline crab migrating from sea to freshwater habitats during the juvenile stage. We used quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the gene expression profile of Na+-K+-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities of 0 ... more

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DDX6; Eriocheir sinensis; gametogenesis; oocyte growth

DDX6 belongs to a family of DEAD-box RNA helicases, which are RNA splicing proteins that ensure the correct folding and structure of mature RNA. Gametogenesis requires the participation of many kinds of RNA. To explore its functions during Eriocheir sinensis gametogenesis, we cloned a full-length DDX6 cDNA sequence from E. sinensis (Es-DDX6) which ... more

Q. Li; Y.L. Wang; J. Xie; W.J. Sun; M. Zhu; L. He; Q. Wang
12/04/2014
Aeromonas hydrophila; APRIL; Aquareovirus; Grass carp; mRNA expression; TWEAK

TWEAK and APRIL are important members of the TNF superfamily, which play a crucial role in several diseases. Here, we describe the identification of grass carp (Ctenopharyngodon idella) homologs of TWEAK and APRIL (designated gcTWEAK and gcAPRIL, respectively) and their response to Aeromonas hydrophila and ... more

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10/31/2014
Aeromonas hydrophila; Biofilm; MSHA pili; MshQ; Pilus; Transposon

Biofilm formation of pathogen bacterium is currently one of the most widely studied topics; however, little is known regarding pathogen bacteria biofilms in aquaculture. Aeromonas hydrophila is a representative species of the genus Aeromonas, which has been recognized as a common pathogen, is associated with many diseases in aquatic animals, and causes significant ... more

Y.X. Qin; Q.P. Yan; X.X. Mao; Z. Chen; Y.Q. Su
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Aeromonas hydrophila; Association analysis; Complement component C7; Grass carp; Single nucleotide polymorphism

Aeromonas hydrophila, a widespread bacterium in the aquatic environment, causes hemorrhagic septicemia in fish. In the last decade, the disease has caused mass mortalities and tremendous economic loss in cultured fish. The complement component C7 is a terminal component of complement that interacts in a sequence of polymerization reactions with other terminal complement ... more

Y.B. Shen; J.B. Zhang; J.J. Fu; X.Y. Xu; J.L. Li; R.Q. Wang; Y.F. Xuan; Y.B. Shen; J.B. Zhang; J.J. Fu; X.Y. Xu; J.L. Li; R.Q. Wang; Y.F. Xuan; Y.B. Shen; J.B. Zhang; J.J. Fu; X.Y. Xu; J.L. Li; R.Q. Wang; Y.F. Xuan
05/09/2016
Aeromonas hydrophila; Detoxifier; Hemerythrin; Macrophages

Survival in host phagocytes is an effective strategy for pathogenic microbes to spread. To understand the mechanisms of Aeromonas hydrophila survival within host macrophages, a library of mini-Tn10 transposon insertion mutants was constructed. The M85 mutant, whose survival in host macrophages was only 23.1% of that of the wild-type (WT) strain, was utilized for further ... more

W.B. Zeng; W.B. Chen; Q.P. Yan; G.F. Lin; Y.X. Qin; W.B. Zeng; W.B. Chen; Q.P. Yan; G.F. Lin; Y.X. Qin
08/15/2014
Cotesia flavipes; Cytochemistry; Diatraea saccharalis; Hemocyte; parasitism; Parasitoid; Ultrastructure

This study describes the morphology of the hemocytes in Diatraea saccharalis larvae during parasitism by the wasp Cotesia flavipes, determined by transmission electron micros­copy using conventional and cytochemical techniques. Twelve-day-old D. saccharalis larvae were naturally infected with the wasp C. flavipes. After different times of parasitism (6 ... more

C.C.C. Nunes; T.L. Silva; E.A. Gregório