Research Article

Related GMR Articles

Bulked segregant analysis; Neotropical fishes; RAPD; Sex determination

Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual ... more

I.G. Almeida; P. Ianella; M.T. Faria; S.R. Paiva; A.R. Caetano
Bulked segregant analysis; Drought tolerance; QTL; SSR markers; Wheat

This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under drought stress ... more

M.R.Naroui Rad; A. Kadir; M.Y. Rafii; H.Z.E. Jaafar; M.R. Naghavi
Amplified fragment length polymorphism; Bulked segregant analysis; Lagerstroemia; Plant architecture; Simple sequence repeat

Plant height is one of the most important traits of plant architecture as it modulates both economic and ornamental values. Crape myrtle (Lagerstroemia indica L.) is a popular ornamental woody plant because of its long-lasting mid-summer bloom, rich colors, and diversified plant architecture. These traits also make it an ideal model of woody species for genetic analysis of many ... more

Y.J. Ye; Y. Liu; M. Cai; D. He; J.S. Shen; Y.Q. Ju; X.M. Bian; H.T. Pan; Q.X. Zhang
Genomic selection; Hundred-seed weight; SCAR markers; Soybean

With the development of molecular marker technology, crop breeding has been accelerated by marker-assisted selection for the improvement of quantitative traits. However, due to the traits' polygenic nature, traditional marker-assisted selection methods are ill-suited for identification of quantitative trait loci. Genomic selection (GS) was introduced into crop breeding to achieve more ... more

Y.J. Shu; D.S. Yu; D. Wang; X. Bai; Y.M. Zhu; C.H. Guo
Drought stress tolerance; Gene expression; Real-time PCR; Soybean; Transcription factor

To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed ... more

S.S. Pereira; F.C.M. Guimarães; J.F.C. Carvalho; R. Stolf-Moreira; M.C.N. Oliveira; A.A.P. Rolla; J.R.B. Farias; N. Neumaier; A.L. Nepomuceno
Esterase polymorphism; Genetic diversity; Soybean

We used native polyacrylamide gel electrophoresis to identify polymorphism levels in a- and b-esterase loci from leaf tissues of Brazilian soybean cultivars for the analysis of population genetic diversity and structure, and to investigate relationships between conventional and genetically modified cultivars. The cultivars included lines developed by a soybean-grower cooperative (CD), by ... more

L.C. Valentini; C.A. Mangolin; S.A.O. Collet; M.F.P.S. Machado
Gene expression; Lignin; Quantitative real-time PCR; seeds; Soybean

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are scarce in the literature. We studied ... more

A. Baldoni; E.V.R. Von Pinho; J.S. Fernandes; V.M. Abreu; M.L.M. Carvalho
AtAREB1ΔQT; Biolistics; Drought tolerance; Soybean; Transcription factor; Water use efficiency

Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AREB1ΔQT) was created. Several ... more

J.P. Leite; E.G.G. Barbosa; S.R.R. Marin; J.P. Marinho; J.F.C. Carvalho; R.F. Pagliarini; A.S. Cruz; M.C.N. Oliveira; J.R.B. Farias; N. Neumaier; F.C.M. Guimarães; T. Yoshida; N. Kanamori; Y. Fujita; K. Nakashima; K.Y. Shinozaki; J.A. Desidério; A.L. Nepomuceno
DDRT; Gene cloning; Glycine max; Heterosis; Soybean

Here, we compare the molecular mechanism of soybean heterosis through the differential expression of basic cloning. Specifically, we cloned 22 differentially expressed cDNA fragments from hybrid combinations of Jilin 38 x EXP (which had obvious yield advantages) and their parents. In addition, we compared the homology of these fragments and predicted their functions. Cloning ... more

J. Zhang; D. Yao; P. Wang; S.Y. Guan; J. Ma; Y.P. Fu
Genetic analysis; Insect herbivory rates; Major gene plus polygene; Soybean

In order to investigate the genetic characteristics of soybean Leguminivora glycinivorella resistance and to improve soybean resistance insectivorous breeding efficiency by applying the multi-generation joint analysis method of the major gene plus polygene model, 5 pedigrees and generations (P1, F1, P2, F2, and F2:3) were used as the materials to perform the soybean L. ... more

J. Zhang; D. Yao; J. Ma; Y.P. Fu; J. Qu; P.W. Wang