Research Article

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quantitative trait locus; Quantitative trait locus-environment interaction; Seed length; Seed width; Soybean

Seed length and seed width are an important factor to the soybean yield. So the quantitative trait loci (QTL) location for seed length and seed width could assistant the breeding of soybean. In this study, the QTL underlying seed length and seed width were studied. A recombinant inbred line population of soybeans derived from a cross between the American semi-draft cultivars Charleston ... more

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Despite the controversy about genetically modified (GM) plants, they are still incrementally cultivated. In recent years, many food and feed products produced by genetic engineering technology have appeared on store shelves. Controlling the production and legal presentation of GM crops are very important for the environment and human health, especially in terms of long-term consumption. ... more

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Abiotic stress; Genetic engineering; GmIMT1gene; Soybean

Here, we aimed to clone and identify the GmIMT1 gene related to the salt stress response in soybean. The full-length cDNA sequence of the GmIMT1 gene was amplified in soybean using degenerate primers of Mesembrythmum crystallium. To understand the stress response, the GmIMT1 gene was cloned and sequenced. Then, the expression vectors of the gene were ... more

H.T. Wang; N. Guo; J.M. Zhao; A. Karthikeyan; D. Xue; C.C. Xue; J.Y. Xu; Z.H. Xu; J.Y. Gai; H. Xing
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Chalcone isomerase; Leguminosae; Positive selection; Protein structure; Soybean

Isoflavonoids and the related synthesis enzyme, chalcone isomerase 1 (CHI1), are unique in the Leguminosae, with diverse biological functions. Among the Leguminosae, the soybean is an important oil, protein crop, and model plant. In this study, we aimed to detect the generation pattern of Leguminosae CHI1. Genome-wide sequence analysis of CHI in 3 Leguminosae and 3 other ... more

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Cysteine-rich polycomb-like protein (CPP-like) genes are a group of transcription factors with highly conserved cysteine-rich domains and are widely distributed in animals and plants, but do not present in yeast. Previous studies have shown that members of this family play important roles in the development of reproductive tissue and in the control of cell division in plants. In ... more

L. Zhang; H.K. Zhao; Y.M. Wang; C.P. Yuan; Y.Y. Zhang; H.Y. Li; X.F. Yan; Q.Y. Li; Y.S. Dong
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cDNA library; Soybean; Soybean regeneration; Suppression subtractive hybridization (SSH)

The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA library constructed from ... more

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Genetic diversity; Grape phylloxera; Population genetics; Simple sequence repeat marker

A genome-wide sequence search was conducted to identify simple sequence repeat (SSR) loci in phylloxera, Daktulosphaira vitifoliae, a major grape pest throughout the world. Collectively, 1524 SSR loci containing mono-, di-, tri-, tetra-, penta-, and hexanucleotide motifs were identified. Among them, trinucleotide repeats were the most abundant in the phylloxera genome ( ... more

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Dendrobium nobile; Expressed sequence tag; Simple sequence repeat marker

Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers could enrich the current resource of molecular markers. In this study, microsatellite markers were developed for Dendrobium nobile Lindl. by mining the ESTs. Twenty-eight EST-SSRs amplified 2 to 6 nucleotide repeats with a mean number of 2.82 alleles per locus. The observed and expected heterozygosities per ... more

J.J. Lu; J.Y. Kang; S.R. Ye; H.Z. Wang
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Carrapateira; Inter-simple sequence repeat marker; Population genetics; Rutaceae; Simple sequence repeat marker; Tropical forest species

Studies of genetic diversity in plant species present in the remaining fragments of the Atlantic Forest are very important for understanding their resilience to such a degraded ecosystem. We analyzed the genetic diversity of 3 populations of the high-density understory species Metrodorea nigra St. Hill. (Rutaceae) located in forest remnants in the region of Ribeirão Preto, SP, ... more

R.M.Moraes Filho; F. Bonifácio-Anacleto; A.L. Alzate-Marin
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genetic mapping; Heterozygosity; Simple sequence repeat marker; Tea cultivars

Genetic maps are essential tools for quantitative trait locus analysis and marker-assisted selection breeding. In order to select parents that are highly heterozygous for genetic mapping, the heterozygosity (HS) of 24 tea cultivars (Camellia sinensis) was analyzed with 72 simple sequence repeat markers. In total, 359 alleles were obtained with an average of 4 ... more

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