Research Article

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Myostatin, encoded by the MSTN gene, is a negative regulator of muscle growth, and its expression level in muscle tissue is closely correlated with muscle growth and satellite cell proliferation. To identify the characteristics of the Pekin duck MSTN gene and the relationship between its polymorphism and breast muscle traits in Pekin duck, cDNA cloning and analysis and ... more

T.S. Xu; L.H. Gu; X.H. Zhang; B.G. Ye; X.L. Liu; S.S. Hou
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Epithelial ovarian cancer; Gene expression; Nuclear factor-kappa B; Polymorphism; β-transducin repeat-containing protein

Epithelial ovarian cancer (EOC) is the leading cause of death among all gynecological cancers. Nuclear factor-kappa B (NF-κB) is involved in carcinogenesis and in the development of EOC. The β-transducin repeat-containing protein (β-TrCP) is a positive regulator of the NF-κB signaling pathway. Recent studies have indicated that the -94 ins/del ATTG polymorphism in the promoter region of ... more

Z.H. Huo; H.J. Zhong; Y.S. Zhu; B. Xing; H. Tang
02/28/2013
Gene expression; Lignin; Quantitative real-time PCR; seeds; Soybean

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are scarce in the literature. We studied ... more

A. Baldoni; E.V.R. Von Pinho; J.S. Fernandes; V.M. Abreu; M.L.M. Carvalho
07/24/2013
Artemisinin; Artesunate; CASP3 and SOD1 genes; Comet assay; Gene expression; HepG2 cells

The malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to evaluate the cytotoxicity and ... more

I. Aquino; M.S.F. Tsuboy; J.C. Marcarini; M.S. Mantovani; F.F. Perazzo; E.L. Maistro
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Gene expression; Kidney; Promoter region; Transcription factor; WNK4

With-no-lysine (K) kinase-4 (WNK4) is a newly cloned kinase-encoding gene that plays a crucial role in the maintenance of electrolyte homeostasis. Mutations of WNK4 can cause pseudohypoal­dosteronism type α, an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension. We explored the expression and regulatory mechanism of WNK4 ... more

Z.J. Sun; Y. Li; Y.H. Li; J.S. Zhang; Q. Ding; J. Li-Ling; Y. Liang; Y.Y. Zhao
07/24/2013
cDNA-AFLP; Floral bud abortion; Gene expression; Radish; Real-time PCR

Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain ... more

J. Zhang; X.L. Sun; L.G. Zhang; M.X. Hui; M.K. Zhang
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BPI gene; Gene expression; Pig; Real-time PCR

We compared and analyzed the expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18 and 35-day expres­sion in the jejunum. There were also significant differences ... more

J. Zhu; C. Zi; Z.C. Wu; L. Liu; X.R. Zheng; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
04/10/2013
Colorectal cancer; DNA hypomethylation; Epigenetics; Gene expression; Metastasis

DNA methylation is a fundamental epigenetic mechanism in regulating the expression of genes controlling crucial cell functions in cancer development. Gene silencing via CpG island methylation/demethylation in the promoter region is one of the mechanisms by which different genes are inactivated/activated in human cancers. Tissue inhibitor of metalloproteinase-2 (TIMP-2) is known to ... more

Z. Hussain; M.I. Khan; M. Shahid; F.N. Almajhdi
04/02/2013
Apoptosis; Bladder cancer; Gene expression; RT-PCR

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder ... more

D.F. Barione; F.S.N. Lizarte; P.C. Novais; C.A.M. de Carvalho; F.C.B. Valeri; F.M. Peria; H.F. de Oliveira; D.L. Zanette; W.A. Silva; A.J. Cologna; R.B. Reis; S. Tucci; A.C.P. Martins; D.P.C. Tirapelli; L.F. Tirapelli
01/24/2013
CTAB-LiCl; Gene expression; Nelumbo nucifera; RNA isolation; RT-PCR

Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-quality RNA ... more

Y.J. Zhang; X.Y. Hao; Z.S. Liang; W.D. Ke; H.B. Guo

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