Research Article

Related GMR Articles

Flowering locus T; Flowering transition; Gene expression; Phalaenopsis orchids; Warm day and cool night conditions

Warm day and cool night conditions significantly induce reproductive spike formation in Phalaenopsis plants; hence, determining the flowering mechanism regulating the reproductive transition is important. Flowering locus T (FT) plays important roles in flowering induction in several plants. To explore spike induction by warm days and cool nights in ... more

D.M. Li; F.B. Lǚ; G.F. Zhu; Y.B. Sun; H.L. Liu; J.W. Liu; Z. Wang
Fertility restoration; Gene expression; Homologous cloning; Male sterility; YABBY gene family

Expression of the YABBY gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. However, the YABBY gene has not been studied in male sterility and fertility restoration. We homologously cloned the CtYABBY1 gene of male-sterile TC1 ... more

X.L. Zhang; L.G. Zhang
Clinical analysis; Exocrine pancreatic insufficiency; Gene expression; Pancreatic disease

Johanson-Blizzard syndrome (JBS) is a rare autosomal recessive disorder resulting from loss-of-function mutations in the UBR1 gene. JBS can be easily recognized by its unique clinical presentation (including exocrine pancreatic insufficiency, hypoplasia/aplasia of the alae nasi, congenital scalp defects, sensorineural hearing loss, growth retardation, psychomotor retardation, and ... more

C.R. Quaio; Y.K. Koda; D.R. Bertola; M. Sukalo; M. Zenker; C.A. Kim
Enzymatic activity; Gene expression; Mangifera indica L.; p-Coumarate 3-hydroxylase; Phenylalanine ammonia lyase

Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar ‘Ataulfo’ in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme activity of PAL and C3H was determined in mango ... more

H. Palafox-Carlos; C.A. Contreras-Vergara; A. Muhlia-Almazán; M.A. Islas-Osuna; G.A. González-Aguilar
Asterias rubens; Gene expression; High-throughput sequencing; Real-time qPCR; Reference gene; UBA52

The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas. This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression at the transcriptional level is estimated by real- ... more

A.F. Sadritdinova; A.A. Dmitriev; A.V. Snezhkina; M.S. Belenikin; G.S. Krasnov; O.G. Manylov; A.A. Kudryavtsev; N.V. Melnikova; A.S. Speranskaya; M.V. Darii; V.A. Lakunina; L.A. Uroshlev; A.O. Smurov; O.A. Stepanov; A.V. Kudryavtseva
Epithelial ovarian cancer; Gene expression; Paraneoplastic tissue; Tumor-supplied group of factors

The objective of this study was to explore the feasibility and methods of screening the residual normal ovarian tissue adjacent to orthotopic ovarian carcinomas in nude mice. Human epithelial ovarian cancer cells (OVCAR3) were subcutaneously implanted for a tumor source and ovarian orthotopic transplantation. The cancer tissue, proximal paraneoplastic tissue, middle paraneoplastic tissue ... more

G.H. Zhu; S.T. Wang; M.Z. Yao; J.H. Cai; C.Y. Chen; Z.X. Yang; L. Hong; S.Y. Yang
Carcass trait; Gene expression; Hu sheep; IGF-I; MyoG

In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.01 < P < 0.05), one-month old (0.01 < P < 0.05), ... more

W. Sun; R. Su; D. Li; H.H. Musa; Y. Kong; J.T. Ding; Y.H. Ma; L. Chen; Y.F. Zhang; W.Z. Wu
5-aminolevunlinic acid; Aquaporin; Cucumber; Gene expression; NaCl stress

Aquaporins play a direct role in plant water relation under salt stress, but the effects of 5-aminolevulinic acid (ALA) on aquaporin gene expression in salt-treated plants remain unknown. This study investigated the potential effects of exogenous ALA (50 mg/dm3) on aquaporin expression levels under salt stress (75 mM NaCl) in the salt-sensitive (Jinchun No.4) and the relatively salt- ... more

F. Yan; D. Qu; Y.Y. Zhao; X.H. Hu; Z.Y. Zhao; Y. Zhang; Z.R. Zou
Gene expression; Gibberellin; Oilseed rape; Reserve accumulation; Seed filling

Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed rape (Brassica napus L.) were treated with GA ... more

X.Q. Huang; R.Q. He; X.Y. Liao; B. Zhou; W.S. Peng; J.Z. Lin; D.Y. Tang; Y.H. Zhu; X.Y. Zhao; X.M. Liu
Capsicum annuum L.; Gene cloning; Gene expression; HsfA2

The heat-shock transcription factor (Hsf) gene CaHsfA2 (GenBank accession No. JX402923) was cloned from the Capsicum annuum thermotolerant line R9 by combining the techniques electron cloning and rapid amplification of cDNA ends. The gene, which is 1436 bp in length, had an open reading frame of 1089 bp that encoded 362 amino acids. There was an 831-bp intron between ... more

M. Guo; Y.X. Yin; J.J. Ji; B.P. Ma; M.H. Lu; Z.H. Gong