Research Article

Related GMR Articles

Gene expression analysis; Id1; mRNA; PBMCs; Proliferation; Psoriasis

Although the precise causes of psoriasis are unclear, it is widely accepted that psoriasis is a disorder in which factors in the immune system, enzymes, and other biochemical substances that regulate skin cell division are impaired, leading to rapid proliferation of keratinocytes and incomplete keratinization. Expression of the helix-loop-helix transcription factor Id1 (inhibitor of ... more

C. Ronpirin; M. Achariyakul; T. Tencomnao; J. Wongpiyabovorn; W. Chaicumpa
Apoptosis; Invasion; miR-181b; PC-3; Proliferation; Prostate cancer

We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC ... more

L. He; H. Yao; L.H. Fan; L. Liu; S. Qiu; X. Li; J.P. Gao; C.Q. Hao
Differentiation; Erythropoietin; Mesenchymal stem cell; Proliferation; Signaling pathways

We examined whether erythropoietin (EPO) can inhibit adipogenic differentiation of mesenchymal stem cells (MSCs) in the mouse bone marrow and its underlying mechanism. We separated and extracted mouse bone marrow MSCs and induced adipogenic differen­tiation using 3-isobutyl-1-methylxanthine, insulin, and dexamethasone. Different concentrations of EPO were added to the cells and observed ... more

G.X. Liu; J.C. Zhu; X.Y. Chen; A.Z. Zhu; C.C. Liu; Q. Lai; S.T. Chen
Esophageal cancer; Flow cytometry; Gene transfection; MT-1E; MT-3; Proliferation

Metallothionein (MT)-3 has cell growth inhibitory activity, and is the only currently known MT subtype with unique physiological functions. The expression levels of MT-1E, a subtype of MT-1, were positively correlated with the degree of esophageal cancer malignancy. The present study aimed to investigate the effects of MT-3 and MT-1E gene transfection on the proliferation, cell cycle, and ... more

Z.Q. Tian; Y.Z. Xu; Y.F. Zhang; G.F. Ma; M. He; G.Y. Wang
NANOG; Neural differentiation; NT2/D1; OCT4; Proliferation; SOX2

SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and ... more

D. Drakulic; A. Krstic; M. Stevanovic
Hypoxia; Osteogenesis; Osteogenic differentiation; Periodontal ligament stem cells; Proliferation

Changes in oxygen concentration may influence various innate characteristics of stem cells. The effects of varying oxygen concentration on human periodontal ligament stem cells (HPDLSCs) has not been explored, particularly under hypoxia-related conditions. First, HPDLSCs were cultured from the periodontium of human teeth using the outgrowth method. STRO-1 and CD146 expression of HPDLSCs ... more

Q.B. Zhang; Z.Q. Zhang; S.L. Fang; Y.R. Liu; G. Jiang; K.F. Li
Anchor-independent growth; Colorectal cancer LoVo cell; ETS-1; LINE-1 ORF-1p; Proliferation; Transcriptional activation

The human proto-oncogene long interspersed nucleotide acid element-1 (LINE-1) open reading frame-1 protein (ORF-1p) is involved in the progress of several cancers. The transcription factor ETS-1 can mediate the transcription of some downstream genes that play specific roles in the regulation of cancerous cell invasion and metastasis. In this study, the effects of LINE-1 ORF-1p on ETS-1 ... more

M.Y. Li; M. Zhu; F. Feng; F.Y. Cai; K.C. Fan; H. Jiang; Z.Q. Wang; E.Q. Linghu
Apoptosis; Aspirin; Proliferation; Thy-1; Wnt/β-catenin

Cancer stem cells have been found to play important roles in carcinoma. Although thy-1 has been identified as a potential stem cell marker of liver cancer, whether the Wnt/β-catenin signaling pathway plays an important role in regulating hepatocarcinoma proliferation and apoptosis mediated by thy-1 remains unknown. Our results showed that high thy-1 expression caused hepG2 cells ... more

B.Q. Cheng; Y. Jiang; Q. Zhu; W.G. Lin
Apoptosis; Cell cycle; Gax; Migration; Proliferation; VSMCs

The Gax gene has been implicated in a variety of cell-developmental and biological processes, and aberrant Gax expression is linked to many diseases. In this study, to provide important insights for Gax-based gene therapy in vein graft restenosis and its anti-restenotic mechanism, we used rabbit vascular smooth muscle cells (VSMCs) to investigate the effects of ... more

H. Zheng; S. Xue; Z.L. Hu; J.G. Shan; W.G. Yang
Apoptosis; Curcumin; Invasion; Melanoma; Migration; Proliferation

Malignant melanoma is a melanocytic tumor with a high potential of invasion and metastasis. Curcumin is extracted from Curcuma longa L.; curcumin has anti-tumor efficacy in multiple systemic malignancies. Here, we investigated the effect of curcumin on A375 human melanoma cells. A375 cells were cultivated, passaged, and treated with different concentrations of curcumin. We ... more

Y.P. Zhang; Y.Q. Li; Y.T. Lv; J.M. Wang